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Pediatric Cardiology

, Volume 38, Issue 5, pp 991–1003 | Cite as

MicroRNAs Association in the Cardiac Hypertrophy Secondary to Complex Congenital Heart Disease in Children

  • Ma. C. Sánchez-Gómez
  • K. A. García-Mejía
  • M. Pérez-Díaz Conti
  • G. Díaz-Rosas
  • I. Palma-Lara
  • R. Sánchez-Urbina
  • M. Klünder-Klünder
  • J. A. Botello-Flores
  • N. A. Balderrábano- Saucedo
  • A. Contreras-Ramos
Original Article

Abstract

Complex congenital heart disease (CHD) affects cardiac blood flow, generating a pressure overload in the compromised ventricles and provoking hypertrophy that over time will induce myocardial dysfunction and cause a potential risk of imminent death. Therefore, the early diagnosis of complex CHD is paramount during the first year of life, with surgical treatment of patients favoring survival. In the present study, we analyzed cardiac tissue and plasma of children with cardiac hypertrophy (CH) secondary to CHD for the expression of 11 miRNAs specific to CH in adults. The results were compared with the miRNA expression patterns in tissue and blood of healthy children. In this way, we determined that miRNAs 1, 18b, 21, 23b, 133a, 195, and 208b constitute the expression profile of the cardiac tissue of children with CHD. Meanwhile, miRNAs 21, 23a, 23b, and 24 can be considered specific biomarkers for the diagnosis of CH in infants with CHD. These results suggest that CH secondary to CHD in children differs in its mechanism from that described for adult hypertrophy, offering a new perspective to study the development of this pathology and to determine the potential of hypertrophic miRNAs to be biomarkers for early CH.

Keywords

MicroRNAs Complex congenital heart Cardiac hypertrophy Children 

Notes

Acknowledgements

This work was supported by Children’s Hospital of Mexico Federico Gómez as part pf HIM/2012/016 SSA 1025 federal grant. Sánchez-Gómez M. C. From Health Science program, Instituto Politécnico Nacional was supported by CoNaCyT MSc (426432) Grant. We thank Lucía Lima from Laboratory of Developmental Biology Research and Experimental Teratogenicity, Children’s Hospital of Mexico Federico Gómez, for technical support on histology.

Funding

Children’s Hospital of Mexico Federico Gómez as part of HIM/2012/016 SSA 1025 federal grant supported this work.

Compliance with Ethical Standards

Conflict of interest

The author Sánchez-Gómez M. C. has received research grants from CoNaCyT MSc (426432). The other authors declare that they have no conflict of interest related this work.

Ethical approval

“All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. For the biopsy bank of the Pathology Department of the HIMFG this type of study formal consent is not required.”

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Ma. C. Sánchez-Gómez
    • 1
    • 5
  • K. A. García-Mejía
    • 1
  • M. Pérez-Díaz Conti
    • 2
  • G. Díaz-Rosas
    • 1
  • I. Palma-Lara
    • 5
  • R. Sánchez-Urbina
    • 1
  • M. Klünder-Klünder
    • 4
  • J. A. Botello-Flores
    • 1
  • N. A. Balderrábano- Saucedo
    • 3
  • A. Contreras-Ramos
    • 1
  1. 1.Laboratory of Developmental Biology Research and Experimental TeratogenicityChildren’s Hospital of Mexico Federico Gomez (HIMFG)Mexico CityMexico
  2. 2.Department of PathologyHIMFGMexico CityMexico
  3. 3.Laboratory of Cardiomyopathies and ArrhythmiasHIMFGMexico CityMexico
  4. 4.Department of Community HealthHIMFGMexico CityMexico
  5. 5.School of MedicineNational Polytechnic Institute (IPN)Mexico CityMexico

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