Effect of Atrazine on Growth, Photosynthesis, and Between-Strain Variability in Scenedesmus subspicatus (Chlorophyceae)

Abstract.

The magnitude of between-strain differences in the sensitivity of algal species to toxicants is not well known. Yet, informations obtained with a single strain are used for ecotoxicological assessment and for interspecific comparisons. Using 12 strains, we determined whether intraspecific variability occurs in the green alga Scenedesmus subspicatus. We examined growth, photosynthetic activity, and short-term sensitivity thereof to the herbicide atrazine (500 μg · L⁻¹) as well as how these responses are affected by chronic exposure to low atrazine concentrations (0, 1, 5, and 20 μg · L⁻¹). Independent of chronic exposure to atrazine, the strains differed in growth rate and photosynthesis rate. Yet the short-term sensitivity to atrazine was affected by the chronic treatments. All but one strain became more tolerant, their short-term sensitivity being inversely related to the applied atrazine concentration and dependent on the duration of exposure. Differences in response to increments in atrazine concentration resulted in differences in rank order of sensitivity of strains. Moreover, between-strain variability was markedly higher in the atrazine treatments than in the controls. These results can be explained by the significant role of genotype versus environment interactions in determining intraspecific differences in adaptive physiological responses of S. subspicatus to chronic exposure to atrazine.