Abstract
Toluene-induced cells of Pseudomonas putida PaW164 (pWWO-164) were monitored for growth potential, maintaining the TOL plasmid, and potential toluene mineralization activity in toluene-amended and nonamended soil. A follow-up study was done in a carbon-free mineral salts solution to obtain further information on physiological changes that occur during starvation. These studies showed that there was a larger decline in colony forming units (CFUs) recovered on a toluate- or benzoate-defined mineral salts medium than on a complex agar medium, a greater percent decrease of CFU than of potential mineralization activity, no decrease in direct counts, and no loss of the TOL plasmid during starvation. Toluene-induced cells also showed an increasing lag time and a decreasing potential for mineralization of 14C-toluene with starvation. In contrast, the lag time for mineralization of glucose was longest at the onset of starvation and reached a minimum by 3 days; thereafter, the potential for glucose mineralization remained high.
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Received: 3 August 2000/Accepted: 3 March 2001
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Lim, T., Gan, KD., Hughes, T. et al. Toluene Mineralization and Growth Potential of Pseudomonas putida PaW164 Under Toluene-Limiting Conditions. Arch. Environ. Contam. Toxicol. 41, 117–122 (2001). https://doi.org/10.1007/s002440010228
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DOI: https://doi.org/10.1007/s002440010228