Abstract
The objective of this study was to evaluate toxicogenetic potential of surface water samples from rivers of center-west Brazil and analyze the influence of land use and cover and physicochemical parameters in genetic damage. Samples were collected during winter (June) and summer (November) at sampling sites from Dourados and Brilhante Rivers (Mato Grosso do Sul/Brazil). The toxicogenetic variables, including chromosomal alterations, micronuclei, and mitotic index, were analyzed in meristematic cells of Allium cepa; and micronuclei, nuclear abnormalities, and DNA strand breaks (arbitrary units, AUT) were analyzed in erythrocytes of Astyanax lacustris. The rivers presented physicochemical values outside the Brazilian laws, which can be a characteristic of human pollution (domestic sewage and local agriculture). The results of A. cepa test suggest that the water samples from Dourados and Brilhante rivers exerted significant (p < 0.05) cytotoxic and genotoxic effects, in both periods of collection, especially alterations in mitotic index. In blood cells of A. lacustris, genotoxic effect of the water samples from the rivers also was observed as significant nuclear abnormalities, DNA breaks (UAT), in both sampling periods, compared with the negative control. Spearman correlation analyses revealed that data of land use and cover and physicochemical parameters were statistically correlated with DNA damages in bioassays. This study demonstrates toxicogenetic potential of water samples from Dourados and Brilhante rivers; furthermore, the type of land use and land cover and physicochemical parameters were revealed to have influence on toxicogenetic damage.
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References
Arcaute CR, Soloneski S, Larramendy ML (2016) Toxic and genotoxic effects of the 2,4-dichlorophenoxyacetic acid (2,4-D)-based herbicide on the Neotropical fish Cnesterodon decemmaculatus. Ecotoxicol Environ Saf 128:222–229. https://doi.org/10.1016/j.ecoenv.2016.02.027
Barbosa JS, Cabral TM, Ferreira DN, Agnez-Lima LF, Medeiros SR (2010) Genotoxicity assessment in aquatic environment impacted by the presence of heavy metals. Ecotoxicol Environ Saf 73:320–325. https://doi.org/10.1016/j.ecoenv.2009.10.008
Batista NJC, Carvalho AA, Cavalcante M, Oliveira MG, Medeiros ECN, Machado JL, Evangelista SR, Dias JF, Santos CEI, Duarte A, Silva FR, Silva J (2016) Genotoxic and mutagenic evaluation of water samples from a river under the influence of different anthropogenic activities. Chemosphere 164:134–141. https://doi.org/10.1016/j.chemosphere.2016.08.091
Bianchi J, Espindola ELG, Marin-Morales MP (2011) Genotoxicity and mutagenicity of water samples from the Monjolinho River (Brazil) after receiving untreated effluents. Ecotoxicol Environ Saf 74:826–833. https://doi.org/10.1016/j.ecoenv.2010.11.006
Boerth DW, Eder E, Stanks JR, Wanek P, Wacker M, Gaulitz S, Skypeck D, Pandolfo D, Yashin M (2008) DNA adducts as biomarkers for oxidative and genotoxic stress from pesticides in crop plants. J Agric Food Chem 56:6751–6760. https://doi.org/10.1021/jf072816q
Bolognesi C, Creus A, Ostrosky-Wegman P, Marcos R (2011) Micronuclei and pesticide exposure. Mutagenesis 26:19–26. https://doi.org/10.1093/mutage/geq070
Bua H, Meng W, Zhang Y, Wan J (2014) Relationships between land use patterns and water quality in the Taizi River basin, China. Ecol Indic 41:187–197. https://doi.org/10.1016/j.ecolind.2014.02.003
Caires EF, Barth G, Garbuio FJ, Churka S (2008) Effects of soil acidity amelioration by surface liming on no-till corn, soybean, and wheat root growth and yield. Eur J Agron 28:57–64. https://doi.org/10.1016/j.eja.2007.05.002
Carrasco KR, Tilbury KL, Myers MS (1990) Assessment of the piscine micronucleus test as an in situ biological indicator of chemical contaminant effects. Can J Fish Sci 47:2123–2136. https://doi.org/10.1139/f90-237
Castro e Sousa JM, Peron AP, da Silva e Sousa L, Holanda MM, Lima AMV, Oliveira VA, da Silva FCC, Lima LHGM, Matos LA, Dantas SMMM, de Aguiar RPS, Islam MT, Melo-Cavalcante AAC, Bonecker CC, Junior HFJ (2017) Cytotoxicity and genotoxicity of Guaribas river water (Piauí, Brazil), influenced by anthropogenic action. Environ Monit Assess 189:1–11. https://doi.org/10.1007/s10661-017-6015-2
Çavas T, Konen S (2007) Detection of cytogenetic and DNA damage in peripheral erythrocytes of goldfish (Carassius auratus) exposed to a glyphosate formulation using the micronucleus test and the comet assay. Mutagenesis 22:263–268. https://doi.org/10.1093/mutage/gem012
Collins AR, Dusinská M (2002) Oxidation of cellular DNA measured with the comet assay. Methods Mol Biol 186:147–159. https://doi.org/10.1385/1-59259-173-6:147
Companhia Tecnológica de Saneamento Ambiental (CETESB) (2005) Relatório de qualidades das águas interiores do estado de São Paulo 2004/CETESB. http://cetesb.sp.gov.br/aguas-interiores/wp-content/uploads/sites/12/2013/11/Cetesb_QualidadeAguasInteriores_2017_02-06_VF.pdf. Accessed 27 Apr 2018
Conselho Nacional do Meio Ambiente (CONAMA) (2005). Resolução número 357, de 17 de março de 2005. http://www.mma.gov.br/port/conama/res/res05/res35705.pdf. Accessed 20 Nov 2017
da Rocha MP, Dourado PLR, Cardoso CAL, Cândido LS, Pereira JG, de Oliveira KMP, Grisolia AB (2018) Tools for monitoring aquatic environments to identify anthropic effects. Environ Monit Assess 190:1–13. https://doi.org/10.1007/s10661-017-6440-2
Dusman E, Luzza M, Savegnago L, Lauxen D, Vicentini VEP, Tonial IB, Sauer TP (2014) Allium cepa L. as a bioindicator to measure cytotoxicity of surface water of the Quatorze River, located in Francisco Beltrão, Paraná, Brazil. Environ Monit Assess 186:1793–1800. https://doi.org/10.1007/s10661-013-3493-8
Environmental Systems Research Institute (ESRI). ArcGIS test GIS for the desktop, versão 10.3. Maps throughout this article were created using ArcGIS® software by Esri trial version. ArcGIS® and ArcMap™ are the intellectual property of Esri. Copyright© Esri. All rights reserved. For more information about Esri® software, please visit www.esri.com, 2015
Ferreira PS, Silva CA (2017) Reclassificação e quantificação da cobertura vegetal nativa da bacia hidrográfica do Rio Brilhante-MS. https://doi.org/10.20396/sbgfa.v1i2017.1828. Accessed 27 Apr 2017
Fiskejo G (1985) The Allium test: a standard in environmental monitoring. Hereditas 102:99–112. https://doi.org/10.1111/j.1601-5223.1985.tb00471.x
Frenzilli G, Nigro M, Lyons BP (2008) The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutat Res 681:80–92. https://doi.org/10.1016/j.mrrev.2008.03.001
Ghisi NC, Ramsdorf WA, Ferraro MVM, Almeida MIM, Ribeiro CAO, Cestari MM (2011) Evaluation of genotoxicity in Rhamdia quelen (Pisces, Siluriformes) after sub-chronic contamination with Fipronil. Environ Monit Assess 180:589–599. https://doi.org/10.1007/s10661-010-1807-7
Ghisi NC, Oliveira EC, Fávaro LF, Assis HCS, Prioli AJ (2014) In situ assessment of a neotropical fish to evaluate pollution in a river receiving agricultural and urban wastewater. Bull Environ Contam Toxicol 93:699–709
Grisolia CK, Starling FLRM (2001) Micronuclei monitoring of fishes from Lake Paranoá, under influence of sewage treatment plant discharges. Mutat Res 491:39–44. https://doi.org/10.1016/S1383-5718(00)00168-6
Helms BS, Schoonover JE, Feminella JW (2009) Assessing influences of hydrology, physicochemistry, and habitat on stream fish assemblages across a changing landscape. J Am Water Resources Assoc 45:157–169. https://doi.org/10.1111/j.1752-1688.2008.00267.x
Houghton DC, Wasson JL (2013) Abrupt biological discontinuity in a small Michigan (USA) stream due to historical riparian canopy loss. J Freshw Ecol 28:293–306. https://doi.org/10.1080/02705060.2013.774298
Instituto Brasileiro de Geografia e Estatística (IBGE) (2017) Instituto Brasileiro de Geografia e Estatística. Censo Demográfico. https://cidades.ibge.gov.br/brasil/ms/dourados/panorama. Accessed 07 Nov 2017
Leme DM, Marin-Morales MA (2009) Allium cepa test in environmental monitoring: a review on its application. Mutat Res 682:71–81. https://doi.org/10.1016/j.mrrev.2009.06.002
Lorente C, Causape J, Glud RN, Hancke K, Merchan D, Muniz S, Val J, Navarro E (2015) Impacts of agricultural irrigation on nearby freshwater ecosystems: the seasonal influence of triazine herbicides in benthic algal communities. Sci Total Environ 503:151–158. https://doi.org/10.1016/j.scitotenv.2014.06.108
Ma TH, Xu Z, Xu C, McConnell H, Rabago EV, Arreola GA, Zhang H (1995) The improved Allium/Vicia root tip micronucleus assay for clastogenicity of environmental pollutants. Mutat Res 334:185–195. https://doi.org/10.1016/0165-1161(95)90010-1
Manzano BC, Roberto MM, Hoshina MM, Menegário AA, Marin-Morales MA (2015) Evaluation of the genotoxicity of waters impacted by domestic and industrial effluents of a highly industrialized region of São Paulo State, Brazil, by the comet assay in HTC cells. Environ Sci Pollut Res 22:1399–1407. https://doi.org/10.1007/s11356-014-3476-5
Marinelli CE, Moretto EM, Brucha G, Lucca JV (2000) Limnologia. In: Espíndola ELG, Silva JSV, Marinelli CE, Abdon MMA (eds) Bacia Hidrográfica do Rio do Monjolinho: uma abordagem ecossistêmica e a visão interdisciplinar. São Carlos, RiMa, pp 133–149
Marotta H, Santos RO, Enrich-Prast A (2008) Monitoramento limnológico: um instrumento para a conservação dos recursos hídricos no planejamento e na gestão urbano-ambientais. Ambiente Soc 11:67–79. https://doi.org/10.1590/S1414-753X2008000100006
Matos LA, Cunha ACS, Sousa AA, Maranhão JPR, Santos NRS, Gonçalves MMC, Dantas SMMM, Sousa JMC, Peron AP, da Silva FCC, de Alencar MVOB, Islam MT, de Aguiar RPS, Melo-Cavalcante AAC, Bonecker CC, Junior HFJ (2017) The influence of heavy metals on toxicogenetic damage in a Brazilian tropical river. Chemosphere 185:852–859. https://doi.org/10.1016/j.chemosphere.2017.07.103
Message HJ, Santos DA, Baumgartner MT, Affonso IP (2016) Planícies de inundação a Biodiversidade do rio Paraná ameaçada. Ciência Hoje 334:36–39
R Core Team (2016) R: a language and environment for statistical computing. R foundation for statistical computing, Vienna. http://www.R-project.org
Radić S, Stipanicev D, Vujcic V, Rajcic MM, Sirac S, Pevalek-Kozlina B (2010) The evaluation of surface and wastewater genotoxicity using the Allium cepa test. Sci Total Environ 408:1228–1233. https://doi.org/10.1016/j.scitotenv.2009.11.055
Scorza Júnior RP, Silva JP (2006) Avaliação do Potencial de Contaminação dos Recursos Hídricos por Pesticidas na Bacia do Rio Dourados, MS. R Ecotoxicol e Meio Ambiente 17:87–106
Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantization of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191. https://doi.org/10.1016/0014-4827(88)90265-0
Slaninova A, Smutna M, Modra H, Svobodova Z (2009) A review: oxidative stress in fish induced by pesticides. Neuro Endocrinol Lett. 1:2–12
Sousa DMG, Lobato E (2004) Cerrado: correção do solo e adubação, 2nd edn. Embrapa, Brasília
Súarez YR, Souza MM, Ferreira FS, Pereira MJ, Silva EA, Ximenes LQL, Azevedo LG, Martins OC, Lima-Junior SE (2011) Patterns of species richness and composition of fish assemblages in streams of the Ivinhema River basin, Upper Paraná River. Acta Limnol Bras 23:177–188. https://doi.org/10.1590/S2179-975X2011000200008
Tabrez S, Shakil S, Urooj M, Damanhouri GA, Abuzenadah AM, Ahmad M (2011) Genotoxicity testing and biomarker studies on surface waters: an overview of the techniques and their efficacies. J Environ Sci Health 29:250–275. https://doi.org/10.1080/10590501.2011.601849
Trujillo-Jiménez P, Sedeño-Díaz JE, Camargo JA, López-López E (2011) Assessing environmental conditions of the Río Champotón (México) using diverse indices and biomarkers in the fish Astyanax aeneus (Günther, 1860). Ecol Indic 11:1636–1646. https://doi.org/10.1016/j.ecolind.2011.04.007
Udroiu I (2006) The micronucleus test in piscine erythrocytes. Aquat Toxicol 79:201–204. https://doi.org/10.1016/j.aquatox.2006.06.013
Vujosević M, Andelković S, Savić G, Blagojević J (2008) Genotoxicity screening of the river Rasina in Serbia using the Allium anaphase-telophase test. Environ Monit Assess 147:75–81. https://doi.org/10.1007/s10661-007-0099-z
Yamamoto FY, Pereira MVM, Lottermann E, Santos GS, Stremel TRO, Doria HB, Gusso-Choueri P, Campos SX, Ortolani-Machado CF, Cestari MM, Filipak Neto F, Azevedo JCR, Oliveira Ribeiro CA (2016) Bioavailability of pollutants sets risk of exposure to biota and human population in reservoirs from Iguaçu River (Southern Brazil). Environ Sci Pollut Res 23:18111–18128. https://doi.org/10.1007/s11356-016-6924-6
Zampella RA, Nicholas AP (2007) Relationship of land-use/land-cover patterns and surface water quality in the Mullica River Basin. J Am Water Resources Assoc 43:594–604. https://doi.org/10.1111/j.1752-1688.2007.00045.x
Zandoná RR, Beutler AN, Burg GM, Barreto CF, Schmidt MR (2015) Gesso e calcário aumentam a produtividade e amenizam o efeito do déficit hídrico em milho e soja. Pesq Agropec Trop 45:128–137. https://doi.org/10.1590/1983-40632015v45303
Acknowledgements
The authors thank the Federal University of Grande Dourados (UFGD) of Mato Grosso do Sul, Brazil for logistical support and the Foundation for Support and Development of Education, Science and Technology of the State of Mato Grosso do Sul (FUNDECT) for financial support.
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Sposito, J.C.V., Francisco, L.F.V., do Amaral Crispim, B. et al. Influence of Land Use and Cover on Toxicogenetic Potential of Surface Water from Central-West Brazilian Rivers. Arch Environ Contam Toxicol 76, 483–495 (2019). https://doi.org/10.1007/s00244-019-00603-2
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DOI: https://doi.org/10.1007/s00244-019-00603-2