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Continuous Exposure to Microplastics Does Not Cause Physiological Effects in the Cultivated Mussel Perna perna

  • Marina F. M. Santana
  • Fabiana T. Moreira
  • Camilo D. S. Pereira
  • Denis M. S. Abessa
  • Alexander Turra
Article

Abstract

The environmental impact of microplastics is a challenging theme, especially under realistic experimental conditions. We investigated physiological responses to 0.1–1.0 μm PVC particles intake by the mussel Perna perna after a relative long-term exposure (90 days) at a less extreme concentration compared with previous studies (0.125 g/L). Microplastic intake was inferred by the presence of PVC in the feces of mussels, and physiological damages were assessed through ingestion rate, assimilation efficiency, growth rate, cellular and molecular biomarkers (lysosomal integrity, lipid peroxidation, and DNA damage), and condition index. All physiological responses showed nonsignificant effects of the microplastics on the exposed mussels. We suggest that, despite the experimental concentration of microplastics, mussels were able to acclimate to the exposure through their abilities for long-term recovery and tolerance to stresses. These data have positive implications for environmental health and in terms of human food resource because mussel farming is a worldwide practice that heavily relies on plastic materials, increasing the chances of microplastic exposure and mussels contamination.

Notes

Acknowledgements

The authors thank CAPES for the student grants, CNPq for the research grant of Denis Abessa (CNPq, 311609/2014-7), Plastivida (Plastics Socio-environmental Institute) for the fellowship to Fabiana Tavares Moreira the technical staff of IOUSP, Liv Ascer and Elisa V. S. Menck for the support throughout the experiment, Prof. Dr. Márcio Reis Custódio for content revision, and Ian McCarthy (FAPESP, 14/21804-3) and Linda Gwen Waters for the language and structure revision.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.

Supplementary material

244_2018_504_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 23 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Marina F. M. Santana
    • 1
    • 2
    • 3
  • Fabiana T. Moreira
    • 1
  • Camilo D. S. Pereira
    • 4
  • Denis M. S. Abessa
    • 5
  • Alexander Turra
    • 1
  1. 1.Department of Biological Oceanography, Oceanographic Institute (IO)University of São Paulo (USP)São PauloBrazil
  2. 2.College of Science and Engineering, Australian Tropical Science and Innovation Precinct (ATSIP)James Cook University (JCU)TownsvilleAustralia
  3. 3.Australian Institute of Marine Science (AIMS)Townsville, Cape ClevelandAustralia
  4. 4.Department of Marine ScienceFederal University of São Paulo (UNIFESP)SantosBrazil
  5. 5.Paulista State University (UNESP)São VicenteBrazil

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