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Long-Term Oil Pollution and In Situ Microbial Response of Groundwater in Northwest China

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Abstract

Potential threats exist where groundwater is polluted by high concentrations of oil compounds (980.20 mg L−1 the highest TPHs). An abandoned petrochemical plant in Lanzhou City, where long-term petrochemical products leakage contaminated the groundwater, was used as a field site in this study. To determine the extent of pollution and find an effective solution, chemical techniques combined with molecular biological techniques were used to survey the migration and decomposition of pollutants. Moreover, Illumina Sequencing was employed to reveal the microbial changes of different sites. Light-chain alkanes (mostly C6–C9), most benzene compounds, and some polycyclic aromatic hydrocarbons (naphthalene, 2-methylnaphthalene) mainly polluted the source. C29 to C36 and chlorobenzenes (hexachlorocyclohexane) polluted the secondary polluted sites. Moreover, chloralkane (trichloroethane and dichloroethane), benzene derivatives (trimethylbenzene and butylbenzene), and PAHs (fluorene and phenanthrene) were present in the other longtime-contaminated water. The bacterial genera are closely related with the chemical matters, and different groups of microorganisms gather in the sample sites that are polluted with different kinds of oil. The biodiversity and abundance of observed species change with pollution conditions. The dominant phyla (81%) of the bacterial community structure are Proteobacteria (62.2% of the total microbes), Bacteroidetes (8.85%), Actinobacteria (6.70%), and Choloroflexi (3.03%). Pseudomonadaceae is significant in the oil-polluted source and Comamonadaceae is significant in the secondary polluted (migrated oil) sample; these two genera are natural decomposers of refractory matters. Amycolatopsis, Rhodocyclaceae, Sulfurimonas, and Sulfuricurvum are the dominant genera in the long-migrated oil-polluted samples. Bioavailability of the oil-contaminated place differs with levels of pollution and cleaning the worse-polluted sites by microbes is more difficult.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant Nos. 51178048, 51378064, and 51678054) and BNU Discretionary Foundation (Grant No. 2014KJJCB22).

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Authors and Affiliations

  1. College of Water Sciences, Beijing Normal University, No 19 Xinwai Street, Beijing, 100875, China

    Yujiao Sun, Sidan Lu, Xiaohui Zhao, Aizhong Ding & Lei Wang

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  1. Yujiao Sun
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  2. Sidan Lu
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  3. Xiaohui Zhao
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  4. Aizhong Ding
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Correspondence to Yujiao Sun.

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Sun, Y., Lu, S., Zhao, X. et al. Long-Term Oil Pollution and In Situ Microbial Response of Groundwater in Northwest China. Arch Environ Contam Toxicol 72, 519–529 (2017). https://doi.org/10.1007/s00244-017-0405-x

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