Effects of CeO2 Nanoparticles on Terrestrial Isopod Porcellio scaber: Comparison of CeO2 Biological Potential with Other Nanoparticles
Nano-sized cerium dioxide (CeO2) particles are emerging as an environmental issue due to their extensive use in automobile industries as fuel additives. Limited information is available on the potential toxicity of CeO2 nanoparticles (NPs) on terrestrial invertebrates through dietary exposure. In the present study, the toxic effects of CeO2 NPs on the model soil organism Porcellio scaber were evaluated. Nanotoxicity was assessed by monitoring the lipid peroxidation (LP) level and feeding rate after 14-days exposure to food amended with nano CeO2. The exposure concentration of 1000 μg of CeO2 NPs g−1 dry weight food for 14 days significantly increased both the feeding rate and LP. Thus, this exposure dose is considered the lowest observed effect dose. At higher exposure doses of 2000 and 5000 μg of CeO2 NPs g−1 dry weight food, NPs significantly decreased the feeding rate and increased the LP level. Comparative studies showed that CeO2 NPs are more biologically potent than TiO2 NPs, ZnO NPs, CuO NPs, CoFe2O4 NPs, and Ag NPs based on feeding rate using the same model organism and experimental setup. Based on comparative metal oxide NPs toxicities, the present results contribute to the knowledge related to the ecotoxicological effects of CeO2 NPs in terrestrial invertebrates exposed through feeding.
KeywordsCeO2 CoFe2O4 Digestive Gland Risk Quotient Cerium Dioxide
The authors thank M. Hočevar for SEM images of nanoparticles taken at the Institute of Metals and Technology in Ljubljana, Slovenia. The present work was funded via the European Commission 7th Framework Programme project “NanoMILE” (contract no. NMP4-LA-2013-310451). A part of work was conducted at Center of excellence in Nanoscience and Nanotechnology, Ljubljana, Slovenia.
- Drobne D (1997) Terrestrial ispods—a good choice for toxicity testing of pollutants in the terrestrial environment. Environ Toxicol Chem 16:1159–1164Google Scholar
- Felix LC, Ortega VA, Ede JD, Goss GG (2013) Physicochemical characteristics of polymer-coated metal-oxide nanoparticles and their toxicological effects on zebrafish (Danio rerio) development. ASC Environ Sci Technol 47:6589–6596Google Scholar
- Novak S, Drobne D, Golobič M, Zupanc J, Romih T, Gianoncelli A, Kiskinova M, Kaulich B, Pelicon P, Vavpetič P, Jeromel L, Ogrinc N, Makovec D (2013) Cellular internalization of dissolved cobalt ions from ingested CoFe2O4 nanoparticles: in vivo experimental evidence. Environ Sci Technol 47:5400–5408CrossRefGoogle Scholar
- Organization for Economic Cooperation and Development (OECD) (2013) OECD Environment, Health and Safety Publications, Series on the Safety of Manufactured Nanomaterials, Document No. 37, Current developments in delegations on the safety of the manufactured nanomaterials—tour de table. ENV/JM/MONO(2013)2Google Scholar
- Šećerov B, Andrić Ž, Abazović N, Krsmanović R, Mitrić M, Montone A, Dramićanin M (2008) Combustion synthesis and characterization of CeO2 nanopowder. Acta Chim Slov 55:486–491Google Scholar
- US EPA (2004) Interim registration eligibility decision. Prevention pesticides and toxic substances (7508C). 738-R-04-006. http://envirocancer.cornell.edu/turf/pdf/diazinon_ired.pdf. Accessed 2 May 2015Google Scholar
- Xia J, Zhao HZ, Lu GH (2013) Effects of selected metal oxide nanoparticles on multiple biomarkers in Carassius auratus. Biomed Environ Sci 26:742–749Google Scholar