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Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats

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Abstract

Improper management of hazardous wastes adversely impacts the environment and the public health. The present study was aimed at investigating the influence of Olushosun municipal landfill leachate (OMLL) from Ojota in the Lagos State of Nigeria on testicular function by assessing the plasma concentrations of reproductive hormones, testicular biometal levels, and antioxidant levels as well as observing the histological alterations in testes and epididymides of rats after exposure to 0, 12.5, and 25 % OMLL in drinking water for 7 days. Exposure to OMLL significantly decreased the daily fluid intake, but it resulted in testicular biometal accumulation as follows: lead > cadmium > nickel > iron > copper. Acute exposure to OMLL induced oxidative stress and increased the activities of marker enzymes of testicular function but markedly decreased the circulatory concentrations of luteinizing hormone, follicle-stimulating hormone, prolactin, testosterone, thyroid-stimulating hormone, triiodothyronine, and thyroxine. Testicular and epididymal degeneration with significant decrease in sperm quality and quantity were observed in OMLL-exposed rats. Collectively, the data presented herein indicate that exposure to OMLL-induced testicular dysfunction associated with biometal accumulation and endocrine disruption in rats. If the effects can be extrapolated to humans, OMLL may present significant health implications for individuals exposed to OMLL-contaminated substances.

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References

  • Adedara IA, Oyebiyi OO, Lawal TA, Adesina AA, Farombi EO (2013) Involvement of oxidative stress in municipal landfill leachate-induced toxicity in boar sperm. Environ Toxicol Pharmacol 36:972–978

    Article  CAS  Google Scholar 

  • Adedara IA, Lawal TA, Adesina AA, Oyebiyi OO, Ebokaiwe AP, Farombi EO (2014) Sperm functional parameters and erythrocytes oxidant-antioxidant imbalance during municipal landfill leachate treatment withdrawal in rats. Environ Toxicol Pharmacol 37:460–467

    Article  CAS  Google Scholar 

  • American Society for Testing and Materials (1992) Standard test method for shake extraction of solid waste with water. ASTM designation: D3987-85. West Conshohocken, PA, ATSM

  • Au A, Ha J, Hernandez M, Polotsky A, Hungerford DS, Frondoza CG (2006) Nickel and vanadium metal ions induced apoptosis of T-lymphocyte Jurkat cells. J Biomed Mater Res A 79:512–521

    Article  Google Scholar 

  • Blazak WF, Trienen KA, Juniewicz PE (1993) Application of testicular sperm head counts in the assessment of male reproductive toxicity. In: Chapin RE, Heindel J (eds) Methods in toxiciology, vol 3A., Male reproductive toxicologyAcademic Press, San Diego, pp 86–94

    Google Scholar 

  • Clairborne A (1995) Catalase activity. In: Greenwald AR (ed) Handbook of methods for oxygen radical research. CRC Press, Boca Raton, pp 237–242

    Google Scholar 

  • Dawson JP, Thayer WW, Desforges JF (1958) Acute hemolytic anaemia in the newborn infant due to naphthalene poisoning: report of two cases with investigations into the mechanism of the disease. Hematology 13:1113–1125

    CAS  Google Scholar 

  • Deguchi Y, Toyoizumi T, Masuda S, Yasuhara A, Mohri S, Yamada M et al (2007) Evaluation of mutagenic activities of leachates in landfill sites by micronucleus test and comet assay using goldfish. Mutat Res 627:178–185

    Article  CAS  Google Scholar 

  • Emenike CU, Fauziah SH, Agamuthu P (2012) Characterization and toxicological evaluation of leachate from closed sanitary landfill. Waste Manag Res 30:888–897

    Article  CAS  Google Scholar 

  • Erkkila K, Aito H, Aalto K, Pentikainen V, Dunkel L (2002) Lactate inhibits germ cell apoptosis in human testes. Mol Hum Reprod 8:109–117

    Article  CAS  Google Scholar 

  • Farombi EO, Tahnteng JG, Agboola AO, Nwankwo JO, Emerole GO (2000) Chemoprevention of 2-acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron—A Garcinia kola seed extract. Food Chem Toxicol 38:535–541

    Article  CAS  Google Scholar 

  • Farombi EO, Adewolo OA, Ajimoko YR (2007) Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in African cat fish (Clarias gariepinus) from Nigerian Ogun river. Int J Environ Res Public Health 4:158–165

    Article  CAS  Google Scholar 

  • Farombi EO, Adedara IA, Ebokaiwe AP, Teberen R, Ehwerhemuepha T (2010) Nigerian Bonny light crude oil disrupts antioxidant systems in testes and sperm of rats. Arch Environ Contam Toxicol 59:166–174

    Article  CAS  Google Scholar 

  • Farombi EO, Akintunde JK, Nzute N, Adedara IA, Arojojoye O (2012) Municipal leachate induces hepatotoxicity and oxidative stress in rats. Toxicol Ind Health 28:532–541

    Article  CAS  Google Scholar 

  • Fauziah SH, Emenike CU, Agamuthu P (2013) Leachate risk and identification of accumulated heavy metals in Pangasius sutchi. Waste Manag Res 31:75–80

    Article  CAS  Google Scholar 

  • Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    CAS  Google Scholar 

  • Hoenig M, de Kersabiec AM (1996) Sample preparation steps for analysis by atomic spectroscopy methods: present status. Spectrochim Acta Part B At Spectrosc 51:1297–1307

    Article  Google Scholar 

  • Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR (1974) Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3,4 bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11:151–169

    Article  CAS  Google Scholar 

  • Krajewska-Kulak E, Sengupta P (2013) Thyroid function in male infertility. Front Endocrinol (Lausanne) 4:174–175

    Google Scholar 

  • Lee GF, Jones-Lee RA (1996) Evaluation of the potential for a proposed or existing landfill to pollute ground-waters. Fred Lee, El Macero, pp 1–18

    Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Maran RRM (2003) Thyroid hormones: their role in testicular steroidogenesis. Arch Androl 49:375–388

    CAS  Google Scholar 

  • Marnett LJ (1999) Lipid peroxidation-DNA damage by malondialdehyde. Mutat Res, Fundam Mol Mech Mutagen 424:83–95

    Article  CAS  Google Scholar 

  • Misra HP, Fridovich I (1972) The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175

    CAS  Google Scholar 

  • Momodu NS, Dimuna KO, Dimuna JE (2011) Mitigating the impact of solid wastes in urban centres in Nigeria. J Hum Ecol 34:125–133

    Google Scholar 

  • Odewabi AO, Ogundahunsi OA, Odewabi AA, Oritogun KS, Ekor M (2013) Adenosine deaminase activity and immunoglobulin levels as potential systemic biomonitors of occupational hazards and health status in municipal solid waste management workers. Environ Toxicol Pharmacol 35:1–12

    Article  CAS  Google Scholar 

  • Okoye BS, Obakin CE, Tongo PS (1991) Heavy metals and organism in the Lagos Lagoon. Int J Environ Stud 37:285–292

    Article  CAS  Google Scholar 

  • Olorunfemi FB (2011) Landfill development and current practices in Lagos metropolis, Nigeria. J Geogr Reg Plan 4:656–663

    Google Scholar 

  • O’Shaughnessy PJ, Monteiro A, Verhoeven G, De Gendt K, Abel MH (2010) Effect of FSH on testicular morphology and spermatogenesis in gonadotrophin-deficient hypogonadal mice lacking androgen receptors. Reproduction 139:177–184

    Article  Google Scholar 

  • Pant N, Srivastava SP (2003) Testicular and spermatotoxic effects of quinalphos in rats. J Appl Toxicol 23:271–274

    Article  CAS  Google Scholar 

  • Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 179:588–590

    Article  CAS  Google Scholar 

  • Sainath SB, Meena R, Supriya C, Reddy KP, Reddy PS (2011) Protective role of Centella asiatica on lead-induced oxidative stress and suppressed reproductive health in male rats. Environ Toxicol Pharmacol 32:146–154

    Article  CAS  Google Scholar 

  • Sanocka D, Kurpisz M (2004) Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2:1–7

    Article  Google Scholar 

  • Schutzendubel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL et al (2001) Cadmium induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol 127:887–898

    Article  CAS  Google Scholar 

  • Siu ER, Mruk DD, Porto CS, Cheng CY (2009) Cadmium-induced testicular injury. Toxicol Appl Pharmacol 238:240–249

    Article  CAS  Google Scholar 

  • Szasz G (1974) Gamma glutamyl transpeptidase. In: Bergmeyer HU (ed) Methods of enzymatic analysis (2nd ed). Academic Press, New York, p. 757

  • Vassault A (1983) Lactate dehydrogenase. UV-method with pyruvate and NADH. In: Bergmeyer HU (ed) Methods of enzymatic analysis (3rd ed). Volume III. New York: Plenum, 1983, pp. 118–125

  • Wagner MS, Wajner SM, Maia AL (2008) The role of thyroid hormone in testicular development and function. J Endocrinol 199:351–365

    Article  CAS  Google Scholar 

  • Wells ME, Awa OA (1970) New technique for assessing acrosomal characteristics of spermatozoa. J Dairy Sci 53:227

    Article  CAS  Google Scholar 

  • Wistuba J, Stukenborg JB, Luetjens CM (2007) Mammalian spermatogenesis. In: Functional development and embryology. Global Science Books 1, pp. 99–117

  • Wolff SP (1994) Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol 233:182–189

    Article  CAS  Google Scholar 

  • Zemjanis R (1970) Collection and evaluation of semen. In: Zemjanis R (ed) Diagnostic and therapeutic technique in animal reproduction, 2nd edn. William and Wilkins, Waverly Press, Baltimore, pp 139–153

    Google Scholar 

Download references

Acknowledgments

This work was supported in part by Multidisciplinary Research Grants under the Staff Training and Research Capacity Building Programme of the John D. and Catherine T. Mac-Arthur Foundation Grant (USA) endowment from the University of Ibadan, Nigeria, awarded to E. O. Farombi. The technical assistance of Omoko Ejiro of the Department of Veterinary Surgery and Reproduction, University of Ibadan, is gratefully appreciated by the authors.

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Authors and Affiliations

  1. Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria

    Isaac A. Adedara, Ifeoluwa O. Awogbindin, Adebayo A. Adesina, Oluwatosin O. Oyebiyi, Tajudeen A. Lawal & Ebenezer O. Farombi

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  1. Isaac A. Adedara
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  2. Ifeoluwa O. Awogbindin
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  3. Adebayo A. Adesina
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  4. Oluwatosin O. Oyebiyi
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  5. Tajudeen A. Lawal
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  6. Ebenezer O. Farombi
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Correspondence to Ebenezer O. Farombi.

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Adedara, I.A., Awogbindin, I.O., Adesina, A.A. et al. Municipal Landfill Leachate-Induced Testicular Oxidative Damage is Associated with Biometal Accumulation and Endocrine Disruption in Rats. Arch Environ Contam Toxicol 68, 74–82 (2015). https://doi.org/10.1007/s00244-014-0075-x

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