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Effects of Bisphenol A and Fadrozole Exposures on cyp19a1 Expression in the Murray Rainbowfish, Melanotaenia fluviatilis

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Abstract

Several endocrine-disrupting chemicals (EDCs) have been attributed to the alteration of reproduction in fish through disrupting endogenous sex steroidogenic pathways including aromatisation of androgens to oestrogen by CYP19 aromatase. Here we investigate this hypothesis in adult male and female Melanotaenia fluviatilis by examining the mRNA expression of cyp19a1 isoforms after exposure for ≤96 h to two EDCs with contrasting modes of action: one a weak oestrogen mimic, bisphenol A [BPA (100 or 500 μg/L)], and the other a nonsteroidal aromatase inhibitor, fadrozole [FAD (10 or 50 µg/L)]. The results suggest that BPA did not affect cyp19a1a expression significantly at both concentrations, whereas 50 µg/L of FAD significantly upregulated its expression in ovary. In contrast, BPA exposures increased expression of cyp19a1b in brain of both males and females, whilst FAD had contrasting effects in brain: It increased in males but decreased in females. Similar contrasting responses of cyp19a1b were induced by BPA in gonads: upregulation in ovary and downregulation in testis. FAD did not have a significant effect on gonadal expression of cyp19a1b. Collectively, the results suggest that BPA and FAD can disrupt cyp19a1b activity more readily than can cyp19a1a, albeit with contrasting effects in either a tissue- or sex-specific context that is conceivably consistent with their (BPA and FAD) opposing modes of action. Enhanced spatial and temporal sensitivity of cyp19a1b compared with cyp19a1a suggests that brain sex of fish is more susceptible to disruption by environmental pollutants such as BPA and FAD. Therefore, we propose that the response of cyp19a1b in brain tissue of M. fluviatilis is a more suitable indicator of oestrogenic pollution in the aquatic environment.

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Acknowledgments

A. H. Shanthanagouda was awarded an RMIT University Scholarship (RUIS). The authors thank anonymous reviewers’ suggestions on the manuscript, which helped to improve the quality of the manuscript.

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Authors and Affiliations

  1. School of Applied Sciences, RMIT University, Bundoora West Campus, Bundoora, VIC, 3083, Australia

    A. H. Shanthanagouda & D. Nugegoda

  2. Inland Fisheries Service Tasmania, New Norfolk, TAS, 7140, Australia

    J. G. Patil

  3. National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, Launceston, TAS, 7250, Australia

    J. G. Patil

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  1. A. H. Shanthanagouda
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  2. D. Nugegoda
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  3. J. G. Patil
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Correspondence to A. H. Shanthanagouda or J. G. Patil.

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Shanthanagouda, A.H., Nugegoda, D. & Patil, J.G. Effects of Bisphenol A and Fadrozole Exposures on cyp19a1 Expression in the Murray Rainbowfish, Melanotaenia fluviatilis . Arch Environ Contam Toxicol 67, 270–280 (2014). https://doi.org/10.1007/s00244-014-0047-1

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