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Cadmium-Resistance Mechanism in the Bacteria Cupriavidus metallidurans CH34 and Pseudomonas putida mt2

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Abstract

Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 were used as cadmium (Cd)-resistant and -sensitive bacteria, respectively, to study Cd uptake, sorption, intracellular accumulation, metallothionein (MT) induction, and bioremediation potential of both isolates. According to this research work, Cd had a stimulatory effect on the growth of CH34 cells (OD578 = 1.43) compared with mt2 cells (OD578 = 0.8). Addition of N,N′-dicyclohexylcarbodiimide (DCCD) and 2,4-dinitrophenol (DNP) along with Cd resulted in more cell growth in mt2 (OD578 = 0.71) compared with CH34 (OD578 = 0.34). DCCD and DNP inhibited this active uptake only in CH34 but not in mt2. Greater Cd interaction with the cell surface was observed in mt2 cells compared with CH34 cells. Intracellular Cd accumulation was interrupted by DCCD and DNP in CH34 (only 1.81 ± 0.04 μg L−1 at 5 h) but not in mt2 (24.41 ± 0.01 μg L−1 at 5 h). Intracellular Cd uptake was observed in even killed mt2 cells (7.11 ± 0.05 μg L−1 at 5 h) compared with CH34 cells (2.50 ± 0.08 μg L−1 at 5 h). This result showed that the Cd accumulation mechanism in CH34 is ATPase-dependent, whereas in mt2 uptake mechanism is not ATPase-dependent because mt2 ATPase was not inhibited by DCCD and DNP. CH34 removed 93 mg L−1 of Cd after 8 days from original industrial effluent, which was more than Cd removal by CH34 from distilled water (i.e. 90 mg L−1 after 8 days). mt2 was able to remove 80 mg L−1 of Cd after 8 days from original industrial effluent, which was more than Cd removal by mt2 from distilled water (i.e. 77 mg L−1 after 8 days). Cd did not induce any MT in CH34, but it did so in mt2 (14 kDa), which was thought to be a Cd-resistance mechanism operative in mt2.

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Acknowledgments

The corresponding author greatly acknowledges DAAD (Deutscher Akademischer Austauschdienst–German Academic Exchange Program). The author conveys special thanks to Sibylle Mothes for providing AAS and ICP-AES facilities and Lukas Y. Wick for providing P. putida mt2 isolate and laboratory facilities at the Department of Environmental Microbiology–UMB, Helmholtz Center for Environmental Research–UFZ, Leipzig, Germany. The technical assistance of Jana Reichenbach (UMB), Ines Volkmann, and Jürgen Steffen at the Department of Analytical Chemistry, Helmholtz Center for Environmental Research–UFZ, are also gratefully acknowledged.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore, 54590, Pakistan

    Saba Shamim & Abdul Rehman

  2. Department of Environmental Microbiology-UMB, Helmholtz Centre for Environmental Research-UFZ, Permoserstrasse 15, 04318, Leipzig, Germany

    Saba Shamim

  3. Institute of Molecular Biology and Biotechnology, The University of Lahore, Defence Road Campus, 1-km, Defence Road, Lahore, Pakistan

    Saba Shamim & Mahmood Hussain Qazi

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  1. Saba Shamim
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Correspondence to Saba Shamim.

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Shamim, S., Rehman, A. & Qazi, M.H. Cadmium-Resistance Mechanism in the Bacteria Cupriavidus metallidurans CH34 and Pseudomonas putida mt2. Arch Environ Contam Toxicol 67, 149–157 (2014). https://doi.org/10.1007/s00244-014-0009-7

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  • DOI: https://doi.org/10.1007/s00244-014-0009-7

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