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Effect of Atrazine, Glyphosate and Quinclorac on Biochemical Parameters, Lipid Peroxidation and Survival in Bullfrog Tadpoles (Lithobates catesbeianus)

  • Michele Flores Dornelles
  • Guendalina Turcato Oliveira
Article

Abstract

Increased use of pesticides worldwide has led to damage not only to natural ecosystems but also to nontarget species. This study assessed the effects of different concentrations of the herbicides atrazine, glyphosate, and quinclorac on biochemical parameters, lipid peroxidation, and survival in tadpoles of Lithobates catesbeianus (bullfrog). Two hundred eighty-eight tadpoles were acquired from a frog farm in the south of Brazil. All animals were kept in aquariums under controlled laboratory conditions for 7 days and exposed to commercial formulations of atrazine (5, 10, and 20 μg/L), glyphosate (36, 72, and 144 μg/L), and quinclorac (0.05, 0.10, and 0.20 μg/L) for 7 days thereafter. The concentrations used in this study are similar to the levels of these herbicides found in natural water bodies. After exposure, gill, liver, and muscle samples were removed from each animal for quantitation of glycogen, total lipids, triglycerides, cholesterol, total proteins, and lipid peroxidation. Atrazine, glyphosate, and quinclorac exposure induced a significant decrease in levels of glycogen and total lipids in gill, liver, and muscle. Triglycerides levels in the gill increased after exposure to glyphosate, and decreased after exposure to atrazine and quinclorac; their levels in liver and muscle decreased on exposure to all herbicides. Cholesterol and total protein levels decreased in liver and muscle for all three herbicides. All tissues exhibited increased lipid peroxidation after exposure to all herbicides. In conclusion, exposure to the herbicides tested in this study induced significant changes in biochemical parameters and increased lipid peroxidation levels in tadpoles of L. catesbeianus.

Keywords

Lipid Peroxidation Atrazine Triglyceride Level Glyphosate Increase Lipid Peroxidation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Dr. Taran Grant, Dr. Nelson Ferreira Fontoura, the team at the PUCRS Laboratory of Conservation Physiology, and CAPES for supporting this study.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Michele Flores Dornelles
    • 1
  • Guendalina Turcato Oliveira
    • 1
    • 2
  1. 1.Laboratório de Fisiologia da Conservação, Departamento de Ciências Morfofisiológicas, Faculdade de BiociênciasPontifícia Universidade Católica Do Rio Grande do SulPorto AlegreBrazil
  2. 2.Bolsista de Produtividade do CNPqBrasiliaBrazil

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