Assessing the Toxicity of Chemical Compounds Associated With Land-Based Marine Fish Farms: The Sea Urchin Embryo Bioassay With Paracentrotus lividus and Arbacia lixula

Abstract

In aquaculture, disinfection of facilities, prevention of fish diseases, and stimulation of fish growth are priority goals and the most important sources of toxic substances to the environment, together with excretory products from fish. In the present study, embryos of two species of sea urchin (Paracentrotus lividus and Arbacia lixula) were exposed to serial dilutions of six antibiotics (amoxicillin (AMOX), ampicillin, flumequine (FLU), oxytetracycline (OTC), streptomycin (ST), and sulfadiazine [SFD]) and two disinfectants (sodium hypochlorite (NaClO) and formaldehyde [CH2O]). Alterations in larval development were studied, and the effective concentrations (ECs) were calculated to evaluate the toxicity of the substances. Both species showed similar sensitivities to all substances tested. Disinfectants (EC50 = 1.78 and 1.79 mg/l for CH2O; EC50 = 10.15 and 11.1 mg/l for NaClO) were found to be more toxic than antibiotics. AMOX, OTC, and ST caused <20 % of alterations, even at the highest concentrations tested. FLU was the most toxic to P. lividus (EC50 = 31.0 mg/l) and SFD to A. lixula (EC50 = 12.7 mg/l). The sea urchin bioassay should be considered within toxicity assessment–monitoring plans because of the sensitivity of larvae to disinfectants.

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Acknowledgments

This study was partly funded by the National Marine Aquaculture Plan. JACUMAR Project (2008): “Selection of indicators, determination of reference values, design of programs, protocols and measures for environmental studies in aquaculture (INDAQUA).” C. Carballeira is grateful for financial support from the University of Cadiz Predoctoral Fellowship Programme (Spain).

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Carballeira, C., De Orte, M.R., Viana, I.G. et al. Assessing the Toxicity of Chemical Compounds Associated With Land-Based Marine Fish Farms: The Sea Urchin Embryo Bioassay With Paracentrotus lividus and Arbacia lixula . Arch Environ Contam Toxicol 63, 249–261 (2012). https://doi.org/10.1007/s00244-012-9769-0

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Keywords

  • NaClO
  • United States Environmental Protection Agency
  • Artificial Seawater
  • AMOX
  • Paracentrotus Lividus