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Impact of the Use of Fluvalinate on Different Types of Beeswax from Spanish Hives

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Abstract

Acaricides are applied in agriculture as phytosanitary products against pests and in apiculture to control the bee parasite Varroa destructor. Poor apicultural practices could result in an accumulation of residues in honeybees, in the environment, and in beeswax and other bee products by migration from the wax comb into stored honey through a process of diffusion and consequently constitute a potential risk for humans. In this study, six different types of beeswax samples were analysed for the determination of residues of fluvalinate, coumaphos, and bromopropylate and its metabolite 4,4′-dibromobenzophenone, all of which are the most commonly acaricides used by Spanish beekeepers against V. destructor. The analytic method consists of solid-phase extraction on a SPE Florisil cartridge and high-performance liquid chromatography separation using a photo diode array detector. The results show that fluvalinate residues were detected in 36.3% of samples, ranging from 1.2 to 6.6 μg/g wax. Residues of coumaphos, bromopropylate, and 4,4′-dibromobenzophenone were not found to be greater than their detection limits. This study indicates that the analysis of these compounds in beeswax samples could be used as bioindicators of fluvalinate sanitary treatment and handling practices applied by beekeepers.

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Acknowledgments

Thanks are given to Dr. Klaus Wallner and his team (Landesanstalt fuer Bienenkunde, Universitaet Hohenheim, Germany); to Spanish Beekeepers, Government of Aragón (Spain) for its financial support (DGA/Grupo de Investigación Consolidado A01/2008); and to DGA/CONSI+D and CAI.

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  1. Department of Animal Production and Food Science, Veterinary Faculty, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain

    Sabine Adamczyk, Regina Lázaro, Consuelo Pérez-Arquillué, Susana Bayarri & Antonio Herrera

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  1. Sabine Adamczyk
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  2. Regina Lázaro
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  3. Consuelo Pérez-Arquillué
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  4. Susana Bayarri
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  5. Antonio Herrera
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Correspondence to Regina Lázaro.

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Adamczyk, S., Lázaro, R., Pérez-Arquillué, C. et al. Impact of the Use of Fluvalinate on Different Types of Beeswax from Spanish Hives. Arch Environ Contam Toxicol 58, 733–739 (2010). https://doi.org/10.1007/s00244-009-9387-7

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  • DOI: https://doi.org/10.1007/s00244-009-9387-7

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