Abstract
Human exposure to methyl parathion can be assessed by measuring the concentration of its metabolite paranitrophenol (PNP) in urine. Our biologic monitoring study in Chiang Mai, Thailand, measured PNP and dialkylphosphate metabolites (i.e., dimethylphosphate [DMP] and dimethylthiophosphate [DMTP]) of methyl parathion in urine samples collected from 136 farmers (age 20 to 65 years) and 306 school children (age 10 to 15 years) in 2006. Participants came from two topographically different areas: one was colder and mountainous, whereas the other was alluvial with climate fluctuations depending on the monsoon season. Both children and farmers were recruited from each area. Despite methyl parathion’s prohibited use in agriculture in 2004, we detected PNP in >90% of all samples analyzed. We applied a nonparametric correlation test (PNP vs. DMP and DMTP) to determine whether the PNP found in most of the samples tested resulted from exposures to methyl parathion. DMP (Spearman’s rho = 0.601 [p = 0.001] for farmers and Spearman’s rho = 0.263 [p <0.001] for children) and DMTP (Spearman’s rho = 0.296 [p = 0.003] for farmers and Spearman’s rho = 0.304 [p<0.001] for children) were positively correlated with PNP, suggesting a common source for the three analytes, presumably methyl parathion or related environmental degradates. Although we found a modest correlation between the metabolites, our findings suggest that despite the prohibition, at least a portion (approximately 25% to 60%) of the PNP detected among farmers and children in Thailand may be attributed to exposure from continued methyl parathion use.
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References
Barr DB, Turner WE, DiPietro E et al (2002) Measurement of p-nitrophenol in the urine of residents whose homes were contaminated with methyl parathion. Environ Health Perspect 110:1085–1091
Bravo R, Caltabiano LM, Weerasekera G et al (2004) Measurement of dialkyl phosphate metabolites of organophosphorus pesticides in human urine using lyophilization with gas chromatography-tandem mass spectrometry and isotope dilution quantification. J Expo Anal Environ Epidemiol 14:249–259
Centers for Disease Control and Prevention (2005) Third national report on human exposure to environmental chemicals. NCEH Publication, Atlanta, GA
Department of Agriculture, Ministry of Agriculture and Cooperatives (2008) Summary of imported pesticides (1994–2006) [in Thai]. Available at: http://www.doa.go.th. Accessed: May 2007
Chemical Safety Platform (2008) Parathion methyl. Available at: http://www.chemtrack.org/Chem-Product.asp?ID=06270 [in Thai]. Accessed: May 2008
Edwards FL, Tchounwou PB (2005) Environmental toxicology and health effects associated with methyl parathion exposure – a scientific review. Int J Environ Res Public Health 2:430–441
Hazardous Substance Act B.E.2535 (1992) Kingdom of Thailand Constitution, Rachakitcha 109:21–43 (in Thai)
Health System Research Institute (2005) Knowledge needed for an effective decision of pesticide management policy in Thailand [in Thai]. Final Report, p. 77
Hernandez F, Sancho JV, Pozo OJ (2004) An estimation of the exposure to organophosphorus pesticides through the simultaneous determination of their main metabolites in urine by liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol 808:229–239
Hornung RW, Reed LD (1990) Estimation of average concentration in the presence of nondetectable values. Appl Occup Environ Hyg 5:46–51
Integrated Pest Management Thailand (2004) Pesticides-health surveys. Available at: http://thailand.ipm-info.org/documents/Health_data_606_(English).pdf, Accessed: May 2008
Morgan DP, Hetzler HL, Slach EF, Lin LL (1977) Urinary excretion of paranitrophenol and alkyl phosphates following ingestion of methyl or ethyl parathion by human subjects. Ach Environ Contam Toxicol 6:159–173
Olsson AO, Baker SE, Nguyen JV et al (2004) A liquid chromatography tandem mass spectrometry multiresidue method for quantification of specific metabolites of organophosphorus pesticides, synthetic pyrethroids, selected herbicides and DEET in human urine. Anal Chem 76:2453–2461
Panuwet P (2009) Assessment of non-persistent pesticide levels in environmentally and occupationally exposed populations. Doctoral Dissertation, Chiang Mai University, Thailand
Panuwet P, Prapamontol T, Chantara S, Olsson AO, Barr DB (2004) A pilot survey of pesticide-specific urinary metabolites among farmers in Chiang Mai highland agricultural area. Chiang Mai University J 3:25–34
Panuwet P, Prapamontol T, Chantara S, Barr DB (2008a) Urinary pesticide metabolites in school students from Northern Thailand. Int J Hyg Environ Health (in press). doi:10.1016/j.ijheh.2008.07.002
Panuwet P, Prapamontol T, Chantara S, Thavornyuthikarn P, Montesano MA, Whitehead RD Jr et al (2008b) Concentrations of urinary pesticide metabolites in small-scale farmers in Chiang Mai Province, Thailand. Sci Total Environ 407:655–668
Perry MJ, Venners SA, Barr DB, Xu X (2007) Environment pyrethroid and organophosphorus insecticide exposure and sperm concentration. Reprod Toxicol 23:113–118
Pesticide Regulatory Subdivision, Ministry of Agriculture and Cooperatives (2008) List of hazardous chemicals prohibited in agriculture. Available at: http://www.pestthai.com [in Thai]. Accessed: February 2008
Ruckart PZ, Kakolewski K, Bove FJ, Kaye WE (2004) Long-term neurobehavioral health effects of methyl parathion exposure in children in Mississippi and Ohio. Environ Health Perspect 112:46–51
Sakellarides TM, Siskos MG, Albanis TA (2002) Photodegradation of selected organophosphorus insecticides under sunlight in different natural waters and soils. Int J Environ Anal Chem 83:33–50
Singh B, Dogra TD, Tripathi CB (2002) A study of serum cholinesterase activity in agricultural and industrial workers occupationally exposed to organophosphates insecticides. Available at: http://ojpms.indianjournals.com/index.php/ijmtlm. Accessed: May 2008
Sriprapat P (2004) Situation of pesticide poisoning among farmers in Eastern Thailand (2002–2004). Disease Prevention Control Office (Region 3), Final Report pp. 20 (in Thai)
Wanfan S (2008) Pesticide free-agriculture and healthy consumers. Thailand Environment Institute. Available at: http://www.tei.or.th/PliBai/th_plibai59_sarakadi.html [in Thai]. Accessed: May 2008
Word Health Organization (1986) Organophosphorus insecticides: a general introduction. WHO, Geneva, Switzerland
Word Health Organization (2004) The WHO recommended classification of pesticides by hazard. WHO, Geneva, Switzerland
Zhao X, Hwang HM (2008) A study of the degradation of organophosphorus pesticides in river waters and the identification of their degradation products by chromatography coupled with mass spectrometry. Arch Environ Contam Toxicol (in press). doi:10.1007/s00244-008-9220-8
Acknowledgments
We thank Dr. John Williamson (CDC/CCID/NCZVED) for his technical advice on statistical analyses. The views presented in this article are those of the authors and do not necessarily reflect the official views of the CDC.
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Panuwet, P., Prapamontol, T., Chantara, S. et al. Urinary Paranitrophenol, a Metabolite of Methyl Parathion, in Thai Farmer and Child Populations. Arch Environ Contam Toxicol 57, 623–629 (2009). https://doi.org/10.1007/s00244-009-9315-x
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DOI: https://doi.org/10.1007/s00244-009-9315-x