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Toxic Effects of Copper on Antioxidative and Metabolic Enzymes of the Marine Gastropod, Onchidium struma

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Abstract

The goals of this study were to evaluate the acute and sublethal toxicity of copper (Cu2+) on the marine gastropod, Onchidium struma, and to examine the utility of enzymatic parameters as indicators of Cu2+ exposure. In a semistatic renewal test, the 96-hour median lethal concentration of Cu2+ for O. struma, 74.80 mg/L, was higher than that for other intertidal species. The activities of the antioxidative enzymes, Cu/Zn superoxide dismutase (Cu/Zn-SOD) and catalase (CAT), and those of the metabolic enzymes-acid phosphatase (ACP), alkaline phosphatase (AKP), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) -in both hepatopancreas and muscle were determined after a 1-week exposure to Cu2+ (range 1.35 to 4.20 mg/L). The activities of both Cu/Zn-SOD and CAT were higher in hepatopancreas than muscle. In addition, there was a negative correlation between Cu2+ concentration and Cu/Zn-SOD activity in hepatopancreas, whereas a positive correlation was observed for CAT activity. Concentration-dependent changes in ACP and AKP activity showed a similar trend in hepatopancreas, increasing then decreasing and, finally, a slight increase. In contrast, ACP activity was positively correlated with Cu2+ across the concentration range tested. In both hepatopancreas and muscle, both GOT and GPT were activated by lower concentrations of Cu2+ and inhibited at higher concentrations.

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Acknowledgments

This project was supported by the Shanghai Key Project for Developing Agriculture by Science and Technology (Grant No. 2005D4-3) and the National Science Foundation of China (Grant No. 30671607).

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Authors and Affiliations

  1. School of Life Science, East China Normal University, 200062, Shanghai, China

    Xiao-bo Li, Xue-li Hou, Qian Mao, Yun-long Zhao & Qun Wang

  2. College of Fisheries and Life Science, Shanghai Ocean University, 200090, Shanghai, China

    Yong-xu Cheng

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  1. Xiao-bo Li
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Correspondence to Qun Wang.

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Li, Xb., Hou, Xl., Mao, Q. et al. Toxic Effects of Copper on Antioxidative and Metabolic Enzymes of the Marine Gastropod, Onchidium struma . Arch Environ Contam Toxicol 56, 776–784 (2009). https://doi.org/10.1007/s00244-009-9290-2

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