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Toxicity of Two Pulsed Metal Exposures to Daphnia magna: Relative Effects of Pulsed Duration-Concentration and Influence of Interpulse Period

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Abstract

Aquatic organisms living in surface waters experience fluctuating contaminant exposures that vary in concentration, duration, and frequency. This study characterized the role of pulsed concentration, pulsed duration, and the interval between pulses on the toxicity of four metals (Cu, Zn, Se, and As) to Daphnia magna. During 21-d toxicity tests, neonatal D. magna were exposed to single or double pulses. Pulsed concentrations and durations ranged from 32 to 6000 µg/L and 8 to 96 h, respectively. Intervals between two pulses ranged from 24 to 288 h. Mortality, growth, and reproduction were characterized for exposures. For single-pulse exposures of Cu and As, metal concentration had a stronger effect on survival of D. magna than did pulsed duration: pulses with 2X concentration and 1Y duration resulted in more mortality than did pulses with 1X concentration and 2Y duration. In contrast, effects of pulsed duration were stronger than metal concentration for Zn. However, the effects of duration and concentration were similar for Se. The relative effects of pulsed concentration and duration found in the present study revealed that the common method using area under the curve (AUC = concentration × duration) may not always accurately estimate environmental risk from metals (e.g., for Cu, Zn, As). In addition, the occurrence of delayed mortality in the present study revealed that using continuous exposure bioassays might underestimate metal toxicity to aquatic biota. For double-pulse exposures, the toxicity of the second pulse was influenced by the first pulse for all four metals. This influence was dependent on the pulsed concentration and duration and the interval between pulses. Further, toxicity caused by the second pulse decreased as the time between the exposures increased. For all four metals, there existed an interval great enough that the toxicity of the two pulses was independent. This would result in less toxicity for multiple exposures than continuous exposures with the same total exposure duration. The interval time at which the effects of the two pulses were independent increased with increasing concentration. Growth and cumulative reproduction of D. magna over 21 d were not significantly affected by pulsed exposures examined in the present study, indicating recovery of the organisms.

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References

  • Allin CJ, Wilson RW (2000) Effects of pre-acclimation to aluminum on the physiology and swimming behavior of juvenile rainbow trout (Oncorhynchus mykiss) during pulsed exposure. Aqua Toxicol 51:213–224

    Article  CAS  Google Scholar 

  • Bossuyt BTA, Janssen CL (2003) Acclimation of Daphnia magna to environmentally realistic copper concentrations. Comp Biochem Physiol C 136:253–264

    Google Scholar 

  • Bossuyt BTA, Janssen CR (2004) Influence of multigeneration acclimation to copper on tolerance, energy reserves, and homeostasis of Daphnia magna straus. Environ Toxicol Chem 23:2029–2037

    Article  CAS  Google Scholar 

  • Brent RN, Herricks EE (1998) Postexposure effects of brief cadmium, zinc, and phenol exposures on freshwater organisms. Environ Toxicol Chem 17:2091–2099

    Article  CAS  Google Scholar 

  • Diamond J. Butcher J, Klaine SJ, Bearr J, Bowersox M, Latimer H, Hoang TC, Zahner H. (2005) Validation of understanding assumption for integrating frequency, magnitude, and duration in permit conditions. Water Environmental Research Foundation Report. Tetra Tech, Inc. MD, USA

    Google Scholar 

  • Diamond JM, Klaine SJ, Butcher JB (2006) Implications of pulsed chemical Exposures for Aquatic Life Criteria and Wastewater Permit Limits. Environ Sci Technol 40:5132–5138

    Article  CAS  Google Scholar 

  • Gorbi GG, Corradi MG, Invidia M, Rivara L, Bassi M (2002) Is Cr(VI) toxicity to Daphnia magna modified by food availability or algal exudates? The hypothesis of a specific chromium/algae/exudates interaction. Water Res 36:1917–1926

    Article  CAS  Google Scholar 

  • Guan R, Wang WX (2004) Cd and Zn uptake kinetics in Daphnia magna in relation to Cd exposure history. Envion Sci Technol 38:6051–6058

    Article  CAS  Google Scholar 

  • Hoang TC, Tomasso JR, Klaine SJ (2007) An integrated model describing the toxic responses of Daphnia magna to pulsed exposures of three metals. Environ Toxicol Chem 26:132–138

    Article  CAS  Google Scholar 

  • Hurd MK, Perry SA, Perry WB (1996) Nontarget effects of a test application of diblubenzuron to forest canopy on stream macroinvertebrates. Environ Toxicol Chem 15:1344–1351

    Article  Google Scholar 

  • Lewis PA, Klemm DJ, Lazorchak JM, Norberg-King TJ, Peltiet WH, Heber MA (1994) Short-term method for estimating the chronic toxicity of effluents and receiving waters to freshwater and marine organisms. EPA 600-/4-91/002. U.S. Environmental Protection Agency, Cincinnati, OH, USA

    Google Scholar 

  • McConnell KP, Roth DM (1962) Se in rat intracellular liver fractions. Biochim Biophys Acta 62:503

    Article  CAS  Google Scholar 

  • McConnell KP (1963) Metalobism of selenium in the mammalian organism. J. Agic Food Chem 11:385

    Article  Google Scholar 

  • Milne I, Seager J, Mallett M, Sims I (2000) Effects of short-term pulsed ammonia exposure on fish. Environ Toxicol Chem 19:2929–2936

    Article  CAS  Google Scholar 

  • Morton MG, Dickson KL, Waller WT, Acevedo MF (2000) Methodology for the evaluation of cumulative episodic exposure to chemical stressor in aquatic risk assessment. Environ Toxicol Chem 19:1213–1221

    Article  CAS  Google Scholar 

  • Muyssen BTA, Janssen CR (2001) Multigeneration zinc acclimation and tolerance in Daphnia magna: Implication for water-quality guidelines and ecological risk assessment. Environ Toxicol Chem 20:2053–2060

    Article  CAS  Google Scholar 

  • Muyssen BTA, Jenssen CR (2002) Accumulation and regulation of zinc in Daphnia magna: Links with homeostasis and toxicity. Arch Environ Contam Toxicol 43:492–496

    Article  CAS  Google Scholar 

  • Naddy RB, Johnson KA, Klaine SJ (2000) Responses of Daphnia magna to pulsed exposures of chlorpyrifos. Environ Toxicol Chem 19:423–431

    Article  CAS  Google Scholar 

  • Naddy RB, Klaine SJ (2001) Effect of pulse frequency and interval on the toxicity of chlorpyrifos to Daphnia magna. Chemosphere 45:497–506

    Article  CAS  Google Scholar 

  • Okazaki RK, Panietz MH (1981) Retention time of twelve trace metals in tissues of the oysters Crassostrea angulata and C. virginica. Mar Biol 63:113–120

    Article  CAS  Google Scholar 

  • Reinert KH, Giddings JM, Judd L (2002) Effects of analysis of time-varying or repeated exposures in aquatic ecological risk assessment of agrochemicals. Environ Toxicol Chem 21:1977–1992

    Article  CAS  Google Scholar 

  • Reynaldi SR, Liess M. Influence of duration of exposure to pyrethroid fenvalerate on sublethal responses and recovery of Daphnia magna straus. Environ Toxicol Chem 24:1160–1164

  • Schultz TW, Freeman SR, Dumont JN (1980) Uptake, depuration, and distribution of selenium in Daphnia and its effects on survival and ultrastrucure. Arch Environ Contam Toxicol 9:23–40

    Article  CAS  Google Scholar 

  • Schulz R, Liess M (2000) Toxicity of fenvalerate to caddisfly larvae: chronic effects of 1- vs 10-h pulse-exposure with constant doses. Chemosphere 41:1511–1517

    Article  CAS  Google Scholar 

  • US Environmental Protection Agency. (1998) Guidelines for ecological risk assessment. Fed Reg 63(93):26846–26924

    Google Scholar 

  • US Environmental Protection Agency. (2004) National Recommended Water Quality Criteria. Office of Water, Office of Science and Technology. Washington DC

    Google Scholar 

  • Van der Hoeven N, Gerritsen AAM (1997) Effects of chlorpyrifos on individuals and populations of Daphnia pulex in the laboratory and field. Environ Toxicol Chem 16:2438–2447

    Article  Google Scholar 

  • Widianarko B, Kuntoro FXS, Van Gestel CAM, Van Straalen NM (2001) Toxicokinetics and toxicity of zinc under time-varying exposure in the guppy (Poecilia reticulata). Environ Toxicol Chem 20:763–768

    Article  CAS  Google Scholar 

  • Zhang L, Baer KN (2000) The influence of feeding, photoperiod and selected solvents on the reproductive strategies of water flea, Daphnia magna. Environ Pollut 110:425–430

    Article  CAS  Google Scholar 

  • Zhao Y, Newman MC (2006) Effects of exposure duration and recovery time during pulsed exposures. Environ Toxicol Chem 25:1298–1304

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the Electric Power Research Institute and the Water Environment Research Foundation.

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Authors and Affiliations

  1. Department of Biological Sciences, Clemson Institute of Environmental Toxicology, 509 Westinghouse Road, Pendleton, South Carolina, 29670, USA

    Tham C. Hoang, Jeffrey S. Gallagher & Stephen J. Klaine

  2. Department of Biology, Texas State University, San Marcos, TX, 78666, USA

    Joseph R. Tomasso

  3. Department of Environmental Studies, Florida International University, C/O Arts & Science, Southeast Environmental Research Center, 3000 NE 151st Street, North Miami, Florida, 33181, USA

    Tham C. Hoang

Authors
  1. Tham C. Hoang
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  2. Jeffrey S. Gallagher
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  3. Joseph R. Tomasso
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  4. Stephen J. Klaine
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Correspondence to Tham C. Hoang.

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Hoang, T.C., Gallagher, J.S., Tomasso, J.R. et al. Toxicity of Two Pulsed Metal Exposures to Daphnia magna: Relative Effects of Pulsed Duration-Concentration and Influence of Interpulse Period. Arch Environ Contam Toxicol 53, 579–589 (2007). https://doi.org/10.1007/s00244-006-0266-1

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