Abstract
Three phenolic compounds were evaluated for their toxicity to the freshwater species Pimephales promelas (fathead minnow) and Ceriodaphnia dubia. Acute toxicity tests using nonylphenol (NP), nonylphenol monoethoxylate (NP1EO), and nonylphenol diethoxylate (NP2EO) were conducted using the single chemicals and as binary and tertiary mixtures. These compounds frequently coexist in surface waters as the result of discharges from wastewater treatment plants that are inefficient at removing nonylphenolic compounds. The mean lethal concentrations (LC50s) for NP, NP1EO, and NP2EO to the fathead minnow were 136, 218, and 323 µg/L, respectively. The LC50s of NP, NP1EO, and NP2EO to C. dubia were 92.4, 328, and 716 µg/L, respectively. The degree of toxic interactions was evaluated by converting mixture LC50 estimates to toxic units (TUs). When exposed to fathead minnows, the binary mixtures of NP plus NP1EO and NP plus NP2EO produced 0.87 and 0.70 TUs, respectively. The tertiary mixture for the same species produced a TU of 0.86. When exposed to C. dubia, the binary mixtures of NP plus NP1EO and NP plus NP2EO produced 0.48 and 1.12 TUs, respectively, whereas the tertiary mixture gave a TU of 0.90. This research suggests that the compounds are additive or synergistic when present in mixtures.
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Ahel M, Molnar E, Ibric S, Giger W (2000) Estrogenic metabolites of alkylphenol polyethoxylates in secondary sewage effluents and rivers. Water Sci Technol 42:15–22
Bailey HC, Elphick JR, Krassoi R, Lovell A (2001) Joint acute toxicity of diazinon and ammonia to Ceriodpahnia dubia. Environ Toxicol Chem 20:2877–2882
Bailey HC, Miller JL, Miller MJ, Wiborg LC, Deanovic L, Shed T (1997) Joint acute toxicity of diazinon and chlorpyrifos to Ceriodaphnia dubia. Environ Toxicol Chem 16:2304–2308
Banks KE, Wood SH, Matthews C, Thuesen KA (2003) Joint acute toxicity of diazinon and copper to Ceriodaphnia dubia. Environ Toxicol Chem 22:1562–1567
Barber LB, Brown GK, Zaugg SD (2000) Potential endocrine disrupting organic chemicals in treated municipal wastewater and river water: In. Keith LH, Jones-Lepp TL, Needham LL (eds) Analysis of environmental endocrine disruptors, American Chemical Society Symposium Series 747. American Chemical Society, Washington, DC, pp 97–123
Belmont AA, Ikonomou M, Metcalfe CD (2006) Presence of nonylphenol ethoxylate surfactants in a watershed in central Mexico and removal from domestic sewage in a treatment wetland. Environ Toxicol Chem 25:29–35
Bettinetti R, Cuccato D, Galassi S, Provini A (2002) Toxicity of 4-nonylphenol in spiked sediment to three populations of Chironomus riparius. Chemosphere 46:201–207
Broderius SJ, Kahl MD, Elonen GE, Hammermeister DE, Hoglund MD (2005) A comparison of the lethal and sublethal toxicity of organic chemical mixtures to the fathead minnow (Pimephales promelas). Environ Toxicol Chem 24:3117–3127
Brooke LT (1993) Acute and chronic toxicity of nonylphenol to ten species of aquatic organisms. Technical report. US Environmental Protection Agency Environmental Research Laboratory, Duluth, MN
Chen CY, Lu CL (2002) An analysis of the combined effects of organic toxicants. Sci Total Environ 289:123–132
Corsi SR, Zitomer DH, Field JA, Cancilla DA (2003) Nonylphenol ethoxylates and other additives in aircraft deicers, antiicers, and waters receiving airport runoff. Environ Sci Technol 37:4031–4037
Ekelund R, Bergman A, Granmo A, Berggren M (1990) Bioaccumulation of 4-nonylphenol in marine animals: a re-evaluation. Environ Pollut 64:107–120
Environment Canada (2000) Priority substances list assessment report nonylphenol and its ethoxylates. Canadian Environmental Protection Act. Draft Report
Franklin NM, Stauber JL, Lim RP, Petocz P (2002) Toxicity of metal mixtures to a tropical freshwater alga (Chlorella sp.): the effect of interactions between copper, cadmium, and zinc on metal cell binding and uptake. Environ Toxicol Chem 21:2412–2422
Kannan K, Keith TL, Naylor GC, Staples CA, Snyder SA, Giesy JP (2003) Nonylphenol and nonylphenol ethoxylates in fish, sediment, and water from the Kalamazoo River, Michigan. Arch Environ Contam Toxicol 44:77–82
Kolpin D, Furlong E, Meyer M, Thurman E, Zaugg S, Barber L, Buxton H (2002) Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999–2000: a national reconnaissance. Environ Sci Technolo 36:1202–1211
Lydy MJ, Linck SL (2003) Assessing the impact of atrazine herbicides on organophosphate insecticide toxicity to the earthworm Eisenia fetida. Arch Environ Contam Toxicol 45:343–349
Merino-Garcia D, Kusk K, Christensen ER (2003). Joint toxicity of similarly and dissimilarly acting chemicals to Daphnia magna at different response levels. Arch Environ Contam Toxicol 45:289–296
Severin GF, Welzel G, Juttner I, Pfister G, Schramm KW (2003) Effects of nonylphenol on zooplankton in aquatic microcosms. Environ Toxicol Chem22:273–2738
Staples CA, Weeks J, Hall JF, Naylor CG (1998) Evaluation of aquatic toxicity and bioaccumulation of C8- and C9-alkylphenol ethoxylates. Environ Toxicol Chem 17:2470–2480
Tanaka J, Nakanishi J (2002) Chronic effects of p-nonylphenol on survival and reproduction of Daphnia galeata: multigenerational life table experiment. Wiley, New York, pp 487–492
US EPA (United States Environmental Protection Agency) (1993) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms. EPA/600/4-90/027F. Environmental Monitoring Systems Laboratory–Cincinnati, Office of Research and Development, Cincinnati, OH\
US EPA (United States Environmental Protection Agency) (2005) Aquatic life ambient water quality criteria–nonylphenol. EPA-822-R-05-005. Final report. United States Environmental Protection Agency, Washington, DC
Weeks JA, Adams JW, Guiney PD, Hall JF, Naylor CG (1996) Risk assessment of nonylphenol and its ethoxylates in U.S. river water and sediment. In: Proceedings of 4th Surfactants Conference, Barcelona Spain. Royal Society of Chemistry, Cambridge, pp 276–291
Yoshimura K (1986) Biodegradation and fish toxicity of nonionic surfactants. J Am Oil Chem Soc 63:1590–1596
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This research was funded by the US Environmental Protection Agency, Office of Research and Development, Region 5.
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TenEyck, M.C., Markee, T.P. Toxicity of Nonylphenol, Nonylphenol Monoethoxylate, and Nonylphenol Diethoxylate and Mixtures of these Compounds to Pimephales promelas (Fathead Minnow) and Ceriodaphnia dubia . Arch Environ Contam Toxicol 53, 599–606 (2007). https://doi.org/10.1007/s00244-006-0249-2
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DOI: https://doi.org/10.1007/s00244-006-0249-2