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Prophylactic effects of quercetin and hyperoside in a calcium oxalate stone forming rat model


Quercetin and hyperoside (QH) are the two main constituents of the total flavone glycosides of Flos Abelmoschus manihot, which has been prescribed for treating chronic kidney disease for decades. This study aimed to investigate the effect of QH on calcium oxalate (CaOx) formation in ethylene glycol (EG)-fed rats. Rats were divided into three groups: an untreated stone-forming group, a QH-treated stone-forming group (20 mg/kg/day) and a potassium citrate-treated stone-forming group (potassium citrate was a worldwide-recognized calculi-prophylactic medicine). Ethylene glycol (0.5 %) was administered to the rats during the last week, and vitamin D3 was force-fed to induce hyperoxaluria and kidney calcium oxalate crystal deposition. 24 h urine samples were collected before and after inducing crystal deposits. Rats were killed and both kidneys were harvested after 3 weeks. Bisected kidneys were examined under a polarized light microscope for semi-quantification of the crystal-formation. The renal tissue superoxide dismutase and catalase levels were measured by Western blot. QH and potassium citrate have the ability to alkalinize urine. The number of crystal deposits decreased significantly in the QH-treated stone-forming group as compared to the other groups. Superoxide dismutase and catalase levels also increased significantly in the QH-treated stone-forming group, as compared with the untreated stone-forming group. QH administration has an inhibitory effect on the deposition of CaOx crystal in EG-fed rats and may be effective for preventing stone-forming disease.

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Fig. 1
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Quercetin and hyperoside


Calcium oxalate


Ethylene glycol


Potassium citrate


Superoxide dismutase


Sodium chloride


Sodium dodecyl sulfate


Polyacrylamide gel electrophoresis


Bovine serum albumin


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This work was partially supported by grants from the National Natural Science Foundation of China (No. 81000311 and No. 81270831).

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The authors declared no potential conflicts of interests with respect to the authorship and/or publication of this article.

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Correspondence to Min Liu or Jun-hua Zheng.

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W. Zhu, Y. Xu and Y. Feng are equal first contributors for this work.

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Zhu, W., Xu, Yf., Feng, Y. et al. Prophylactic effects of quercetin and hyperoside in a calcium oxalate stone forming rat model. Urolithiasis 42, 519–526 (2014).

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  • Nephrolithiasis
  • Calcium oxalate
  • Quercetin
  • Hyperoside
  • Oxidative stress