Abstract
Our study was designed to investigate the protective effect of the COX-2 inhibitor, celecoxib, on renal tubules against shock wave lithotripsy (SWL). Sprague–Dawley rats were randomly divided into three groups: sham, control, and COX-2 groups. The control group was administrated normal saline. The COX-2 group was administered celecoxib (10 mg/kg). After administration for 1 week, the control and COX-2 groups received 1,000 shock waves. Before and after SWL, 24-h urine was collected. CCr was measured to assess renal function. To determine the renal tubular injury, N-acetyl glucosaminidase (NAG) and β-2 microglobulin levels in urine were quantified. The COX-2 gene expression was compared between the three groups. Prior to SWL, all groups had similar levels of NAG and β-2 microglobulin. After SWL, all groups showed similar CCr. Compared with the sham group, control and COX-2 groups produced increase of NAG and β-2 microglobulin excretion. However, NAG and β-2 microglobulin excretions were significantly lower in the COX-2 group than control group. The COX-2 gene expression did not increase in the sham group. However, the COX-2 gene expression was significantly increased in the control group, which was prevented by celecoxib in COX-2 group. Biochemical findings supported a renal protective effect of celecoxib on SWL. This study suggests that celecoxib would be useful prior and after SWL because of renal protective effects.
This is a preview of subscription content, access via your institution.
References
Jeong BC, Park HK, Kwak C, Oh SJ, Kim HH (2005) How painful are shockwave lithotripsy and endoscopic procedures performed at outpatient urology clinics? Urol Res 33:291–296
Miyajima A, Ito K, Asano T, Seta K, Ueda A, Hayakawa M (2001) Does cyclooxygenase-2 inhibitor prevent renal tissue damage in unilateral ureteral obstruction? J Urol 166:1124–1129
Kawata K, Takeyoshi I, Iwanami K, Sunose Y, Tsutsumi H, Ohwada S, Matsumoto K, Morishita Y (2003) The effects of a selective cyclooxygenase-2 inhibitor on small bowel ischemia-reperfusion injury. Hepatogastroenterology 50:1970–1974
Ozturk H, Gezici A, Ozturk H (2006) The effect of celecoxib, a selective COX-2 inhibitor, on liver ischemia/reperfusion-induced oxidative stress in rats. Hepatol Res 34:76–83
Feitoza CQ, Câmara NO, Pinheiro HS, Gonçalves GM, Cenedeze MA, Pacheco-Silva A, Santos OF (2005) Cyclooxygenase 1 and/or 2 blockade ameliorates the renal tissue damage triggered by ischemia and reperfusion injury. Int Immunopharmacol 5:79–84
Aksoy H, Aksoy Y, Turhan H, Keleş S, Ziypak T, Ozbey I (2007) The effect of shock wave lithotripsy on nitric oxide and malondialdehyde levels in plasma and urine samples. Cell Biochem Funct 25:533–536
Li X, He D, Zhang L, Cheng X, Sheng B, Luo Y (2006) A novel antioxidant agent, astragalosides, prevents shock wave-induced renal oxidative injury in rabbits. Urol Res 34:277–282
Willis LR, Evan AP, Connors BA, Shao Y, Blomgren PM, Pratt JH, Fineberg NS, Lingeman JE (2005) Shockwave lithotripsy: dose-related effects on renal structure, hemodynamics, and tubular function. J Endourol 19:90–101
Rubin JI, Arger PH, Pollack HM, Banner MP, Coleman BG, Mintz MC, Van Arsdalen KN (1987) Kidney changes after shock wave lithotripsy: CT evaluation. Radiology 162:21–24
Shao Y, Connors BA, Evan AP, Willis LR, Lifshitz DA, Lingeman JE (2003) Morphological changes induced in the pig kidney by extracorporeal shock wave lithotripsy: nephron injury. Anat Rec A Discov Mol Cell Evol Biol 275:979–989
Ferreira U, Claro Jde A, Rodrigues Netto N, Denardi F Jr, Figueiredo JF, Riccetto CL (1995) Functional and histologic alterations in growing solitary rat kidney as result of extracorporeal shockwaves. J Endourol 9:45–49
Akdaş A, Türkeri LN, Ilker Y, Simşek F, Emerk K (1994) Short-term bioeffects of extracorporeal shockwave lithotripsy. J Endourol 8:187–190
Jan CR, Chen WC, Wu SN, Tseng CJ (1998) Nifedipine, verapamil and diltiazem block shock-wave-induced rises in cytosolic calcium in MDCK cells. Chin J Physiol 41:181–188
Sarica K, Yencilek F (2008) Prevention of shockwave induced functional and morphological alterations: an overwiew. Arch Ital Urol Androl 80:27–33
Strohmaier WL, Billes IC, Abelius A, Lahme S, Bichler KH (2002) Selenium reduces high energy shock wave-induced renal injury in rats. Urol Res 30:31–34
Kitahara M, Eitner F, Ostendorf T, Kunter U, Janssen U, Westenfeld R, Matsui K, Kerjaschki D, Floege J (2002) Selective cyclooxygenase-2 inhibition impairs glomerular capillary healing in experimental glomerulonephritis. J Am Soc Nephrol 13:1261–1270
Reichman J, Cohen S, Goldfarb M, Shina A, Rosen S, Brezis M, Karmeli F, Heyman SN (2001) Renal effects of nabumetone, a COX-2 antagonist: impairment of function in isolated perfused rat kidneys contrasts with preserved renal function in vivo. Exp Nephrol 9:387–396
Dey A, Maric C, Kaesemeyer WH, Zaharis CZ, Stewart J, Pollock JS, Imig JD (2004) Rofecoxib decreases renal injury in obese Zucker rats. Clin Sci 107:561–570
Fujihara CK, Antunes GR, Mattar AL, Andreoli N, Malheiros DM, Noronha IL, Zatz R (2003) Cyclooxygenase-2 (COX-2) inhibition limits abnormal COX-2 expression and progressive injury in the remnant kidney. Kidney Int 64:2172–2182
Acknowledgment
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2007-331-E00151).
Author information
Authors and Affiliations
Corresponding author
Additional information
An erratum to this article can be found at http://dx.doi.org/10.1007/s00240-010-0267-4
Rights and permissions
About this article
Cite this article
Park, H.K., Lee, H.W., Lee, K.S. et al. Preventive effects of COX-2 inhibitor, celecoxib on renal tubular injury induced by shock wave lithotriptor. Urol Res 38, 223–228 (2010). https://doi.org/10.1007/s00240-009-0243-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00240-009-0243-z
Keywords
- Kidney
- COX-2 inhibitors
- Extracorporeal shockwave lithotripsy