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Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino

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Abstract

Urocalun®, a herbal medicine prepared from an extract of Quercus salicina Blume/Quercus stenophylla Makino (QS extract), has been clinically used for the treatment of urolithiasis in Japan since 1969. In the present study, the effects of QS extract on oxalate-induced cell injury and NADPH-induced superoxide anion (O2 −) production in the injured cells were investigated. Oxalate-induced cell injury was assessed by mitochondrial reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide and leakage of lactate dehydrogenase into the extracellular fluid. When NRK-52E cells were injured by exposure to oxalate for 24 h, QS extract prevented the injury in a dose-dependent manner. In addition, QS extract suppressed the increase in NADPH-induced O2 − production, or NADPH oxidase activity, in the homogenate of cells injured by oxalate exposure. These findings suggest that the reduction in oxalate-induced O2 − production contributes to the cytoprotective effect of QS extract.

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Authors and Affiliations

  1. Department of Urogenital Surgery, Kanazawa Medical University, Uchinada, Ishikawa, Japan

    Manabu T. Moriyama, Katsuhito Miyazawa & Koji Suzuki

  2. Research Laboratories, Nippon Shinyaku Co., Ltd, 14, Nishinosho-monguchi-cho, Kisshoin, Minami-ku, Kyoto, Japan

    Kumiko Noda, Michiko Oka & Mitsushi Tanaka

Authors
  1. Manabu T. Moriyama
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  2. Katsuhito Miyazawa
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  3. Kumiko Noda
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  4. Michiko Oka
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  5. Mitsushi Tanaka
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  6. Koji Suzuki
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Correspondence to Manabu T. Moriyama.

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Moriyama, M.T., Miyazawa, K., Noda, K. et al. Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino. Urol Res 35, 295–300 (2007). https://doi.org/10.1007/s00240-007-0114-4

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  • DOI: https://doi.org/10.1007/s00240-007-0114-4

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