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Oxalate synthesis from hydroxypyruvate in vitamin-B6-deficient rats

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Abstract

We studied the effects of an intravenous hydroxypyruvate load on endogenous oxalogenesis in rats receiving a standard diet or a vitamin-B6-deficient diet. Twelve male Wistar rats were randomized to two groups and were fed either a standard diet or a vitamin-B6-deficient diet for 3 weeks. Then the animals received an intravenous infusion of 100 mg/ml (960.6 μmol/ml) of hydroxypyruvate slowly over 10 min. Urine samples were collected just before hydroxypyruvate infusion and at hourly intervals until 5 h afterward. Urinary oxalate, glycolate, and citrate levels were measured by capillary electrophoresis. Hourly urinary oxalate excretion peaked within 2 h, while urinary glycolate excretion peaked at 1 h, after the hydroxypyruvate load in both control and vitamin-B6-deficient rats. Both urinary oxalate and glycolate excretion were higher in vitamin-B6-deficient rats than in control rats. Infusion of hydroxypyruvate increased the 5-h urinary oxalate and glycolate excretion to 0.68% (6.56 μmol) and 0.53% (5.10 μmol) of the administered dose (mol/mol), respectively, in the control rats, while oxalate and glycolate excretion, respectively, increased to 2.43% (23.36 μmol) and 0.79% (7.59 μmol) of the dose in the vitamin-B6-deficient rats. Urinary citrate excretion was significantly lower at baseline and all other times in the vitamin-B6-deficient rats than in the control rats. In conclusion, a hydroxypyruvate load increased endogenous oxalate synthesis in control rats, and its synthesis was even greater in vitamin-B6-deficient rats. Vitamin B6 deficiency also resulted in significant hypocitraturia.

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Authors and Affiliations

  1. Division of Urology, Department of Organ-oriented Medicine, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa, 903-0215, Japan

    Yaovalak Teerajetgul, Rayhan Zubair Hossain, Kenichi Yamakawa, Makoto Morozumi, Kimio Sugaya & Yoshihide Ogawa

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  1. Yaovalak Teerajetgul
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  2. Rayhan Zubair Hossain
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  3. Kenichi Yamakawa
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  4. Makoto Morozumi
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  5. Kimio Sugaya
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  6. Yoshihide Ogawa
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Correspondence to Yoshihide Ogawa.

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Teerajetgul, Y., Hossain, R.Z., Yamakawa, K. et al. Oxalate synthesis from hydroxypyruvate in vitamin-B6-deficient rats. Urol Res 35, 173–178 (2007). https://doi.org/10.1007/s00240-007-0102-8

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  • DOI: https://doi.org/10.1007/s00240-007-0102-8

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