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Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis

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Abstract

8-hydroxydeoxyguanosine (8-OHdG) is an oxidatively modified guanosine, which has been widely used as an oxidative DNA damage marker in various diseases. The present study aimed to determine urinary 8-OHdG in nephrolithiasis patients and evaluate its clinical significance. Thirty-six nephrolithiasis patients and 30 healthy subjects were recruited. Urine volume, creatinine, malondialdehyde, β-N-acetylglucosaminidase (NAG) activity and proteins were measured in 24 h urine samples. Urinary 8-OHdG was determined by competitive enzyme-linked immunosorbent assay. Mineral composition of stones was analyzed using Fourier-transformed infrared spectroscopy. Nephrolithiasis patients excreted urinary 8-OHdG significantly higher than healthy controls. Urinary 8-OHdG levels compared among patients with calcium oxalate, struvite and uric acid stones were insignificantly different. The urinary NAG activity correlated positively with urinary 8-OHdG. Multiple linear regression showed that urinary NAG activity was an independent predictor of urinary 8-OHdG level. Receiver operating characteristic analysis revealed that the urinary 8-OHdG test was adequate for diagnosing nephrolithiasis. At 10 μg/g creatinine cutoff, the 8-OHdG test imparted high specificity (96.67%) and a positive predictive value (91.67%). In conclusion, this is the first report of elevated urinary 8-OHdG excretion in nephrolithiasis patients indicating increased oxidative DNA damage. Increased renal tubular damage was independently associated with elevated urinary 8-OHdG. Elevated urinary 8-OHdG levels adjunct with metabolic profile may be useful for identifying people at risk of stone development.

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Acknowledgments

This study was supported by grants from the Graduate School, Chulalongkorn University and from the Division of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University. The authors are grateful to Professor Yoshihide Ogawa and Dr. Rayhan Zubair Hossain for generous advice and technical assistance. We would like to thank Professor Henry Wilde and Mettanando Bhikkhu for English correction. Also, we are thankful to Dr. Pisit Prapunwatana for comments.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Rama IV Rd, Bangkok, 10330, Thailand

    Chanchai Boonla, Rattiporn Wunsuwan & Piyaratana Tosukhowong

  2. Biochemistry and Molecular Biology of Metabolic Diseases Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand

    Chanchai Boonla & Piyaratana Tosukhowong

  3. Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand

    Kriang Tungsanga

Authors
  1. Chanchai Boonla
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  2. Rattiporn Wunsuwan
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  3. Kriang Tungsanga
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  4. Piyaratana Tosukhowong
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Correspondence to Piyaratana Tosukhowong.

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Boonla, C., Wunsuwan, R., Tungsanga, K. et al. Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis. Urol Res 35, 185–191 (2007). https://doi.org/10.1007/s00240-007-0098-0

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  • DOI: https://doi.org/10.1007/s00240-007-0098-0

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