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The role of cholinesterases in rat urinary bladder contractility

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Abstract

This study examines the effects of inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) on acetylcholine (ACh)-induced contraction in rat urinary bladder smooth muscle. Neostigmine, a non-selective ChE inhibitor, caused concentration-dependent contractions in rat urinary bladder strips, whereas tetraisopropylpyrophosphoramide (iso-OMPA; a BuChE inhibitor) failed to affect the resting tone of the preparations. Neostigmine (1 µM) markedly augmented the contractile responses to ACh. Although iso-OMPA (10 µM) also potentiated ACh-induced contraction, the effect was less than that evoked by neostigmine. The activities of AChE in rat urinary bladder strips were significantly (P<0.05) higher than those of BuChE. These results indicated that AChE, rather than BuChE, plays an important role in controlling ACh-induced contractions of rat urinary bladder.

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Authors and Affiliations

  1. Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan

    Tsutomu Nakahara, Yuko Kubota, Kenji Sakamoto & Kunio Ishii

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  1. Tsutomu Nakahara
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  2. Yuko Kubota
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  3. Kenji Sakamoto
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  4. Kunio Ishii
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Nakahara, T., Kubota, Y., Sakamoto, K. et al. The role of cholinesterases in rat urinary bladder contractility. Urol Res 31, 223–226 (2003). https://doi.org/10.1007/s00240-003-0326-1

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  • DOI: https://doi.org/10.1007/s00240-003-0326-1

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