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Effects of ovariectomy and hormone replacement on collagen and blood vessels of the urethral submucosa of rats

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Abstract

Collagen and blood vessels of the urethral submucosa of ovariectomized rats were studied following 28 daily subcutaneous injections of 17-ß estradiol (n=6, group 1), medroxy-progesterone acetate (n=6, group 2), both drugs (n=6, group 3) or vehicle (n=6, control) and after sham surgery without castration or injection (n=6). Investigations included the immunohistochemistry of estrogen and progesterone receptors and collagen fibres, Western blot analysis of collagen types I and III and counting periurethral vessels by light microscopy. Our results showed positive immunostaining with estrogen, progesterone and collagen types I and III in all samples. Collagen type I and III levels were lower in the controls than in the sham group. The other groups showed increases (2>3>1) over the controls with a relatively higher increase in type III. The type I/III collagen ratio progressively decreased (control>1>2>3) below sham levels. The mean vessel count was significantly lower in control than in sham animals (P<0.00001). However, only estrogen treatment significantly increased the vessel number compared to controls (P=0.04). Our results indicate that estrogen and progesterone, alone or in combination, have an effect on collagen types I and III, and that estrogen has an effect on blood vessels of the urethral submucosa in female rats.

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References

  1. Baskin LS, Constantinescu SC, Howard PS, McAninch JW, Ewalt DH, Duckett JW, Snyder HM, Macark EJ (1993) Biochemical characterization and quantitation of the collagenous components of urethral sricture tissue. J Urol 150:642

    CAS  PubMed  Google Scholar 

  2. Bergman A, Elia G, Cheung D, Perelman N, Nimni ME (1994) Biochemical composition of collagen in continent and stress urinary incontinent women. Obstet Gynecol Invest 37:48

    CAS  Google Scholar 

  3. Cardozo LD, Kelleher CJ (1995) Sex hormones, the menopause and urinary problems. Gynecol Endocrinol 9:75

    CAS  PubMed  Google Scholar 

  4. Chaliha C, Kullar V (2000) Urinary incontinence: epidemiology and aetiology. Curr Obstet Gynaecol 10:60

    Article  Google Scholar 

  5. Chen BH, Wen Y, Li H, Polan ML (2002) Collagen metabolism and turnover in women with stress urinary incontinence and pelvic prolapse. Int Urogynecol J 13:80

    Article  Google Scholar 

  6. DiBella ZI, Girao MJ, Sartori MF, DiBella V, Lederman HM, Baracat EC, Lima GR (2000) Power Doppler of the urethra in continent or incontinent, pre- and postmenopausal women. Int Urogynecol J 11:148

    Article  Google Scholar 

  7. Endo RM, Girao MJ, Sartori MG, Simoes MJ, Baracat EC, De Lima GR (2000) Effect of estrogen-progesterone hormone replacement therapy on periurethral blood vessels. Int Urogynecol J 11:120

    Article  CAS  Google Scholar 

  8. Falconer C, Ekman G, Malmstrom A, Ulmsten U (1994) Decreased collagen synthesis in stress-incontinent women. Obstet Gynecol 84:583

    CAS  PubMed  Google Scholar 

  9. Falconer C, Ekman-Ordeberg G, Ulmsten U, Westergren-Thorsson G, Barchan K, Malmstrom A (1996) Changes in paraurethral connective tissue at menopause are counteracted by estrogen. Maturitas 24:197

    Google Scholar 

  10. Gosling J (1979) The structure of the bladder and urethra in relation to function. Urol Clin North Am 6:31

    CAS  PubMed  Google Scholar 

  11. Hextall A, Cardozo L (2001) The role of estrogen supplementation in lower urinary tract dysfunction. Int Urogynecol J 12:258

    Article  CAS  Google Scholar 

  12. Hextall A, Bidmead J, Cardozo L, Hooper R (2001) The impact of the menstrual cycle on urinary symptoms and the results of urodynamic investigation. Br J Obstet Gynaecol 108:1193

    Article  CAS  Google Scholar 

  13. Huisman AB (1983) Aspects on the anatomy of the female urethra with special relation to urinary continence. Contrib Gyecol Obstet 10:1

    CAS  Google Scholar 

  14. Jackson SR, Averty NC, Tarlton JF, Eckford SD, Abrams P, Bailey AJ (1996) Changes in metabolism of collagen in genitourinary prolapse. Lancet 347:1658

    CAS  PubMed  Google Scholar 

  15. Jackson SR, James M, Abrams P (2002) The effect of oestradiol on vaginal collagen metabolism in postmenopausal women with genuine stress incontinence. Br J Obstet Gynaecol 109:339

    Article  CAS  Google Scholar 

  16. Keane DP, Sims TJ, Abrams P, Bailey AJ (1997) Analysis of collagen status in premenopausal nulliparous women with genuine stress incontinence. Br J Obstet Gynaecol 104:994

    CAS  PubMed  Google Scholar 

  17. Kim JC, Yoon JY, Seo S, Hwang TK, Park YH (2000) Effects of partial bladder outlet obstruction and its relief on types I and III collagen and detrusor contractility in the rat. Neurourol Urodyn, 19:29

    Google Scholar 

  18. Levin RM, Malkowics SB, Wein AJ (1984) Laboratory models for neuropharmacologic studies. World J Urol 2:222

    Google Scholar 

  19. Liapis A, Bakas P, Pafiti A, Frangos-Plemenos M, Arnoyannaki N, Creatsas G (2001) Changes of collagen type III in female patients with genuine stress incontinence and pelvic floor prolapse. Eur J Obstet Gynecol Reprod Biol 97:76

    Article  CAS  PubMed  Google Scholar 

  20. Norton PA (1993) Pelvic floor disorders: the role of fascia and ligaments. Clin Obstet Gynecol 36:926

    CAS  PubMed  Google Scholar 

  21. Otto LN, Slayden OD, Clark AL, Brenner RM (2002) The rhesus macaque as an animal model for pelvic organ prolapse. Am J Obstet Gynecol 186:416

    Article  PubMed  Google Scholar 

  22. Rechberger T, Donica H, Baranowski W, Jakowicki J (1993) Female urinary stress incontinence in terms of connective tissue biochemistry. Eur J Obstet Gynecol Reprod Biol 49:187

    CAS  PubMed  Google Scholar 

  23. Rechberger T, Postawski K, Jakowicki JA, Gunja-Smith Z, Woessner JF (1998) Role of fascial collagen in stress urinary incontinence. Am J Obstet Gynecol 179:1511

    CAS  PubMed  Google Scholar 

  24. Rizk DEE, Raaschou T, Mason N, Berg B (2001) Evidence of progesterone receptors in the mucosa of the urinary bladder. Scand J Urol Nephrol 35:305

    Article  CAS  PubMed  Google Scholar 

  25. Sartori MG, Girao MJ, Baracat EC, de Lima GR, Sartori JP, Simoes MJ (1998) Quantification of the collagen and muscular fibres in the urethra and in the urinary bladder of castrated female rats and under reposicao hormonal. Int Urogynecol J 9:339

    Google Scholar 

  26. Siracusano S, Bertolotto M, D'Aloia G, Silvestre G, Stener S (2001) Colour Doppler ultrasonography of female urethral vascularization in normal young volunteers: a preliminary report. BJU Int 88:378

    Article  CAS  PubMed  Google Scholar 

  27. Tapp AJS, Cardozo L (1986) The postmenopausal bladder. Br J Hosp Med 35:20

    CAS  PubMed  Google Scholar 

  28. Tsai EM, Yang CH, Chen HS, Wu CH, Lee JN (2001) Bladder neck circulation by Doppler ultrasonography in postmenopausal women with urinary stress incontinence. Obstet Gynecol 98:52

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates

    Diaa E. E. Rizk

  2. Department of Anatomy, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates

    Eric P. Mensah-Brown, Ijaz Ahmed & Mahmoud Al-Haj

  3. Department of Pharmacology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates<

    Swaminathan I. Chandranath, Mohamed Shafiullah & Abdu Adem

  4. Department of Biochemistry, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates

    Mahendra Patel

  5. P.O. Box 17666, Al-Ain, United Arab Emirates

    Diaa E. E. Rizk

Authors
  1. Diaa E. E. Rizk
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  2. Eric P. Mensah-Brown
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  3. Swaminathan I. Chandranath
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  4. Ijaz Ahmed
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  5. Mohamed Shafiullah
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  6. Mahendra Patel
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  7. Mahmoud Al-Haj
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  8. Abdu Adem
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Correspondence to Diaa E. E. Rizk.

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Rizk, D.E.E., Mensah-Brown, E.P., Chandranath, S.I. et al. Effects of ovariectomy and hormone replacement on collagen and blood vessels of the urethral submucosa of rats. Urol Res 31, 147–151 (2003). https://doi.org/10.1007/s00240-003-0306-5

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  • DOI: https://doi.org/10.1007/s00240-003-0306-5

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