Phylogeny and Evolution of RNA 3′-Nucleotidyltransferases in Bacteria
The tRNA nucleotidyltransferases and poly(A) polymerases belong to a superfamily of nucleotidyltransferases. The amino acid sequences of a number of bacterial tRNA nucleotidyltransferases and poly(A) polymerases have been used to construct a rooted, neighbor-joining phylogenetic tree. Using information gleaned from that analysis, along with data from the rRNA-based phylogenetic tree, structural data available on a number of members of the superfamily and other biochemical information on the superfamily, it is possible to suggest a scheme for the evolution of the bacterial tRNA nucleotidyltransferases and poly(A) polymerases from ancestral species. Elements of that scheme are discussed along with questions arising from the scheme which can be explored experimentally.
KeywordsCCA end tRNA Nucleotidyltransferase Poly(A) polymerase Phylogeny Alien index Horizontal gene transfer Polynucleotide phosphorylase
The author thanks Dr. Shozo Yokoyama for his advice on the phylogenetic analysis and Drs. Yokoyama and Paul Joyce for critical readings of this manuscript. Some of the research described herein was supported by Grant Number MCB-0133520 from the National Science Foundation.
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