Speciation proceeds by the accumulation of DNA differences in time. The genetic code changes as a result of genetic drift and by selective pressure. In variable domains, exposure to high selective pressure obscures the view on background mutations. Therefore, we characterized and visualized background mutations using the highly conserved collagen 1α1 GXY domain. Typical change routes were identified and the data set showed several indications that changes in the collagen 1α1 GXY domain have taken place randomly within a functionally restricted space. The types of nucleotide and codon group differences are similar across the vertebrate subphylum and gradually become less functionally neutral with increasing distance between species, which offers the opportunity for rapid visualization of evolutionary relations using a single domain. It was concluded that the findings and approach of the study could be important for analytical method development in authenticity research, especially when conserved domains are targeted.
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The study was a sequel to a previous proteogenomic study (Kleinnijenhuis and van Holthoon 2018) and was financed by Triskelion.
Conflict of interest
The author declares no conflicts of interest. The work was financed by Triskelion.
Handling editor: Konstantinos Voskarides.
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Kleinnijenhuis, A.J. Visualization of Genetic Drift Processes Using the Conserved Collagen 1α1 GXY Domain. J Mol Evol 87, 106–130 (2019). https://doi.org/10.1007/s00239-019-09890-8
- Genetic drift
- Collagen 1α1
- Molecular evolution
- Coding DNA
- Functional restriction