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Journal of Molecular Evolution

, Volume 76, Issue 3, pp 83–97 | Cite as

The 5S rDNA High Dynamism in Diplodus sargus is a Transposon-Mediated Mechanism. Comparison with Other Multigene Families and Sparidae Species

  • Manuel A. Merlo
  • Ismael Cross
  • Manuel Manchado
  • Salvador Cárdenas
  • Laureana Rebordinos
Article

Abstract

There has been considerable discussion in recent years on the evolution of the tandemly repeated multigene families, since some organisms show a concerted model whereas others show a birth-and-death model. This controversial subject extends to several species of fish. In this study, three species of the Sparidae family (Pagrus pagrus, P. auriga and Diplodus sargus) and an interspecific hybrid (P. pagrus (♀) × P. auriga (♂)) have been studied at both molecular and cytogenetic level, taking three different multigene families (5S rDNA, 45S rDNA and U2 snDNA). Results obtained with the 5S rDNA in P. pagrus and P. auriga are characterized by a considerable degree of conservation at the two levels; however, an extraordinary variation was observed in D. sargus at the two levels, which has never been found in other fishes studied to date. As a consequence of this, the evolutionary model of the multigene families is discussed considering the results obtained and others from the bibliography. The result obtained in the hybrid allowed the recombination frequency in each multigene family to be estimated.

Keywords

Multigene family Sparidae Hybrids Concerted evolution Birth-and-death evolution 

Notes

Acknowledgments

This study has been supported by the AQUAGENET project (SOE2/P1/E287), INTERREG IVB SUDOE program, and by grants from the Junta de Andalucía (Spain) to the PAI BIO-219 group.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

239_2013_9541_MOESM1_ESM.doc (1.8 mb)
Supplementary material 1 (DOC 1893 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Manuel A. Merlo
    • 1
  • Ismael Cross
    • 1
  • Manuel Manchado
    • 2
  • Salvador Cárdenas
    • 2
  • Laureana Rebordinos
    • 1
  1. 1.Laboratorio de Genética, Facultad de Ciencias del Mar y AmbientalesUniversidad de CádizCádizSpain
  2. 2.Centro IFAPA, Junta de AndaluciaCádizSpain

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