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The Absence of TIR-Type Resistance Gene Analogues in the Sugar Beet (Beta vulgaris L.) Genome

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Abstract

The majority of known plant resistance genes encode proteins with conserved nucleotide-binding sites and leucine-rich repeats (NBS-LRR). Degenerate primers based on conserved NBS-LRR motifs were used to amplify analogues of resistance genes from the dicot sugar beet. Along with a cDNA library screen, the PCR screen identified 27 genomic and 12 expressed NBS-LRR RGAs (nlRGAs) sugar beet clones. The clones were classified into three subfamilies based on nucleotide sequence identity. Sequence analyses suggested that point mutations, such as nucleotide substitutions and insertion/deletions, are probably the primary source of diversity of sugar beet nlRGAs. A phylogenetic analysis revealed an ancestral relationship among sugar beet nlRGAs and resistance genes from various angiosperm species. One group appeared to share the same common ancestor as Prf, Rx, RPP8, and Mi, whereas the second group originated from the ancestral gene from which 12C1, Xa1, and Cre3 arose. The predicted protein products of the nlRGAs isolated in this study are all members of the non-TIR-type resistance gene subfamily and share strong sequence and structural similarities with non-TIR-type resistance proteins. No representatives of the TIR-type RGAs were detected either by PCR amplification using TIR type-specific primers or by in silico screening of more than 16,000 sugar beet ESTs. These findings suggest that TIR type of RGAs is absent from the sugar beet genome. The possible evolutionary loss of TIR type RGAs in the sugar beet is discussed.

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Acknowledgements

This work was financially supported by the EU (FAIR6-CT08-4235) and the DFG (CA220/2-2). Dr. Fan Longjian thanks the Christian-Albrechts-University, Kiel, Germany for travel grants. We also thank Ms. C. Thiele for technical assistance and Dr. Nikki LeBrasseur for critical reading of the manuscript.

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  1. Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Kiel, Germany

    Yanyan Tian, Tim Thurau, Christian Jung & Daguang Cai

  2. Institute of Bioinformatics, Huajiachi Campus, Zhejiang University, Hangzhou 310029, PR China

    Longjiang Fan

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Tian, Y., Fan, L., Thurau, T. et al. The Absence of TIR-Type Resistance Gene Analogues in the Sugar Beet (Beta vulgaris L.) Genome . J Mol Evol 58, 40–53 (2004). https://doi.org/10.1007/s00239-003-2524-4

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