Abstract
We have sampled a large number of plant taxa, ranging from brown algae to angiosperms, for the presence of myosin sequences. Using phylogenetic analysis, we show that all but two of the new plant myosin sequences fall into two of three preexisting myosin classes. We identified two outlying sequences, which do not fall into any preexisting myosin class. Additionally, all genomic sequences encoding class XI myosins contain an intron in the region studied, suggesting that this genomic region has been conserved over at least 1 billion years of plant evolution. With these data, we can rapidly and consistently classify partial myosin sequences from plants. Our data show that plant myosins do not have clear orthologues in other kingdoms, providing interesting insights into the diversification of myosins.
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References
SF Altschul W Gish W Miller EW Myers DJ Lipman (1990) ArticleTitleBasic local alignment search tool. J Mol Biol 215 403–104 Occurrence Handle10.1006/jmbi.1990.9999 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D Occurrence Handle2231712
InstitutionalAuthorNameArabidopsis Genome Initiative project (2000) ArticleTitleAnalysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408 796–815 Occurrence Handle11130711
WM Bement T Hasson JA Wirth RE Cheney MS Mooseker (1994a) ArticleTitleCorrection: Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types. Proc Natl Acad Sci USA 91 11767c
WM Bement T Hasson JA Wirth RE Cheney MS Mooseker (1994b) ArticleTitleIdentification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types. Proc Natl Acad Sci USA 91 6549–6553 Occurrence Handle1:CAS:528:DyaK2cXlt1Klsrc%3D
JS Berg BC Powell RE Cheney (2001) ArticleTitleA millennial myosin census. Mol Biol Cell 12 780–794 Occurrence Handle1:CAS:528:DC%2BD3MXjtVaqs7k%3D Occurrence Handle11294886
C Burge S Karlin (1997) ArticleTitlePrediction of complete gene structures in human genomic DNA. J Mol Biol 268 78–94 Occurrence Handle10.1006/jmbi.1997.0951 Occurrence Handle1:CAS:528:DyaK2sXjtlSqtL4%3D Occurrence Handle9149143
JJ Doyle JL Doyle (1987) ArticleTitleA rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19 11–15
W Gish DJ States (1993) ArticleTitleIdentification of protein coding regions by database similarity search. Nat Genet 3 266–272 Occurrence Handle1:CAS:528:DyaK3sXhvVCmsb4%3D Occurrence Handle8485583
N Goldman S Whelan (2000) ArticleTitleStatistical tests of gamma-distributed rate heterogeneity in models of sequence evolution in phylogenetics. Mol Biol Evol 17 975–978 Occurrence Handle1:CAS:528:DC%2BD3cXjvFygsLw%3D Occurrence Handle10833204
M Hasegawa H Kishino T Yano (1985) ArticleTitleDating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22 160–74 Occurrence Handle1:CAS:528:DyaL2MXmtFSns7g%3D Occurrence Handle3934395
S Henikoff JG Henikoff (1992) ArticleTitleAmino acid substitution matrices from protein blocks. Proc Natl Acad Sci USA 89 10915–10919 Occurrence Handle1:CAS:528:DyaK3sXjsFCgsQ%3D%3D
T Hodge MJ Cope (2000) ArticleTitleA myosin family tree. J Cell Sci 113 3353–3354 Occurrence Handle1:CAS:528:DC%2BD3cXnvF2gsLk%3D Occurrence Handle10984423
DT Jones WR Taylor JM Thornton (1992) ArticleTitleThe rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci 8 275–282 Occurrence Handle1:CAS:528:DyaK38Xlt1Okt7w%3D Occurrence Handle1633570
AJ Kim SA Endow (2000) ArticleTitleA kinesin family tree. J Cell Sci 113 3681–3682 Occurrence Handle1:CAS:528:DC%2BD3cXosVGmtL4%3D Occurrence Handle11034894
DL Kirk (1998) Volvox: Molecular-genetic origins of multicellularity and cellular differentiation. Cambridge University Press New York
L Liu J Zhou TC Pesacreta (2001) ArticleTitleMaize myosins: Diversity, localization, and function. Cell Motil Cytoskeleton 48 130–148 Occurrence Handle10.1002/1097-0169(200102)48:2<130::AID-CM1004>3.3.CO;2-P Occurrence Handle1:CAS:528:DC%2BD3MXht1Wks7k%3D Occurrence Handle11169765
T Muller M Vingron (2000) ArticleTitleModeling amino acid replacement. J Comput Biol 7 761–767
KM Pryer H Schneider AR Smith R Cranfill PG Wolf JS Hunt SD Sipes (2001) ArticleTitleHorsetails and ferns are a monophyletic group and the closest living relatives to seed plants. Nature 409 618–622 Occurrence Handle10.1038/35054555 Occurrence Handle1:STN:280:DC%2BD3M7islSntw%3D%3D Occurrence Handle11214320
AS Reddy IS Day (2001) ArticleTitleAnalysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence. Genome Biol 2 RESEARCH0024 Occurrence Handle1:STN:280:DC%2BD3MvntF2gtw%3D%3D
N Saitou M Nei (1987) ArticleTitleThe neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 Occurrence Handle1:STN:280:BieC1cbgtVY%3D Occurrence Handle3447015
JR Sellers (2000) ArticleTitleMyosins: A diverse superfamily. Biochim Biophys Acta 1496 3–22 Occurrence Handle10.1016/S0167-4889(00)00005-7 Occurrence Handle1:CAS:528:DC%2BD3cXhslamu78%3D Occurrence Handle10722873
T Soldati H Geissler EC Schwarz (1999) ArticleTitleHow many is enough? Exploring the myosin repertoire in the model eukaryote Dictyostelium discoideum. Cell Biochem Biophys 30 389–411 Occurrence Handle1:CAS:528:DyaK1MXmt1yjs7g%3D Occurrence Handle10403058
K Strimmer A von Haeseler (1997) ArticleTitleLikelihood-mapping: A simple method to visualize phylogenetic content of a sequence alignment. Proc Natl Acad Sci USA 94 6815–6819 Occurrence Handle1:CAS:528:DyaK2sXktF2ruro%3D Occurrence Handle9192648
DL Swofford (2002) PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates Sunderland, MA
K Tamura M Nei (1993) ArticleTitleEstimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10 512–526 Occurrence Handle1:CAS:528:DyaK3sXks1CksL4%3D Occurrence Handle8336541
InstitutionalAuthorNameTAP Group (1998) ArticleTitleAn ordinal classification for the families of flowering plants. Ann Mo Bot Gard 85 531–553
JD Thompson DG Higgins TJ Gibson (1994) ArticleTitleCLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 4673–4680 Occurrence Handle7984417
O Vugrek B Moepps (2002) ArticleTitleHamy3, a novel type 100 kDa myosin from sunflower. J Exp Bot 53 769–771 Occurrence Handle10.1093/jexbot/53.369.769 Occurrence Handle1:CAS:528:DC%2BD38XitlCksLs%3D Occurrence Handle11886898
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Bezanilla, M., Horton, A.C., Sevener, H.C. et al. Phylogenetic Analysis of New Plant Myosin Sequences . J Mol Evol 57, 229–239 (2003). https://doi.org/10.1007/s00239-003-2469-7
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DOI: https://doi.org/10.1007/s00239-003-2469-7