Abstract
Analysis of the AMP-forming ACS gene was performed in 12 species of the Drosophilidae family. Systematically four introns, aligned at the same positions, were detected, but none of them showed a position similar to those known for species outside the Drosophilidae family. The average length of introns varied from 63 to 75 bp but in two species Drosophila takahashii and D. kikkawai the length of the second intron was 343 and 210 bp, respectively. In coding regions, about 80% of the third codon positions were substituted while first and second positions showed, respectively, 14% and 6% substitutions. Interestingly, the divergence observed at the protein level between species was very low. The phylogenetic tree based on the DNA sequences of the exons was mainly in agreement with taxonomic classification and previous molecular phylogenies except for D. ananassae, which appeared more closely related to D. subobscura and D. funebris than to the species of the melanogaster group.
Similar content being viewed by others
References
IR Bock MR Wheeler (1972) ArticleTitleThe Drosophila melanogaster species group. Univ Texas Publs 7 1–102
M Chakir O Peridy P Capy E Pla JR David (1993) ArticleTitleAdaptation to alcoholic fermentation in Drosophila: A parallel selection imposed by environmental ethanol and acetic acid. Proc Natl Acad Sci USA 90 3621–3625 Occurrence Handle1:CAS:528:DyaK3sXktVarurg%3D Occurrence Handle8475110
JL Da Lage M Wegnez ML Cariou (1996) ArticleTitleDistribution and evolution of introns in Drosophila amylase genes. J Mol Evol 43 334–347 Occurrence Handle1:CAS:528:DyaK28XmtFais78%3D Occurrence Handle8798339
R DeSalle (1992) ArticleTitleThe phylogenetic relationships of flies in the family Drosophilidae deduced from mtDNA sequences. Mol Phyl Evol 1 31–40 Occurrence Handle1:CAS:528:DyaK3sXitlSmur0%3D
M Deutsch M Long (1999) ArticleTitleIntron-exon structures of eukaryotic model organisms. Nucleic Acids Res 27 3219–3228 Occurrence Handle10.1093/nar/27.15.3219 Occurrence Handle1:CAS:528:DyaK1MXlt1eks78%3D Occurrence Handle10454621
N Galtier M Gouy C Gautier (1996) ArticleTitleSEAVIEW and PHYLO_WIN: Two graphic tools for sequence alignment and molecular phylogeny. Comput Applic Biosci 12 543–548 Occurrence Handle1:CAS:528:DyaK2sXhtlWktLw%3D
Gilbert DG (1998) Seqpup: A biosequence editor (Version 8.0c). Distributed by the author at seqpup@bio.indiana.edu
N Inomata H Tachida T Yamazaki (1997a) ArticleTitleMolecular evolution of the Amy multigenes in the subgenus Sophophora of Drosophila. Mol Biol Evol 14 1338 Occurrence Handle1:CAS:528:DyaK1cXivFKnsA%3D%3D
N Inomata H Tachida T Yamazaki (1997b) ArticleTitleMolecular evolution of the Amy multigenes in the subgenus Sophophora of Drosophila. Mol Biol Evol 14 942–950 Occurrence Handle1:CAS:528:DyaK2sXlvVejurc%3D
N Iwai N Ohmichi K Hanai Y Nakamura M Kinoshita (1994) ArticleTitleHuman SA gene locus as a candidate locus for essential hypertension. Hypertension 23 375–380 Occurrence Handle1:CAS:528:DyaK2MXovVWn Occurrence Handle7907320
D Karan JR David P Capy (2001) ArticleTitleMolecular evolution of the AMP-forming acetyl-CoA synthetase. Gene 265 95–101 Occurrence Handle10.1016/S0378-1119(01)00358-4 Occurrence Handle1:CAS:528:DC%2BD3MXhsleksb4%3D Occurrence Handle11255012
D Lachaise ML Cariou JR David F Lemeunier L Tsacas M Ashburner (1988) ArticleTitleHistorical biogeography of the Drosophila melanogaster species subgroup. Evolution Biol 22 159–225
JT Lis JA Simon CA Sutton (1983) ArticleTitleNew heat shock puffs and beta-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene. Cell 35 403–410 Occurrence Handle1:CAS:528:DyaL2cXmt1Ohtw%3D%3D Occurrence Handle6418386
JM Logsdon (1998) ArticleTitleThe recent origins of spliceosomal introns revisited. Curr Op Gen Dev 8 637–648 Occurrence Handle10.1016/S0959-437X(98)80031-2 Occurrence Handle1:CAS:528:DyaK1MXhs1yjuw%3D%3D
M Long M Deutsch (1999) ArticleTitleAssociation of intron phase with conservation at spliced sequences and evolution of spliceosomal introns. Mol Biol Evol 16 1528–1534 Occurrence Handle1:CAS:528:DyaK1MXntlKjsrk%3D Occurrence Handle10555284
M Long SJ de Souza C Rosenberg W Gilbert (1998) ArticleTitleRelationship between ‘‘proto-splice sites’’ and intron phases: Evidence from dicodon analysis. Proc Natl Acad Sci USA 95 219–223 Occurrence Handle10.1073/pnas.95.1.219 Occurrence Handle1:CAS:528:DyaK1cXjtl2jsQ%3D%3D Occurrence Handle9419356
M Long C Rosenberg W Gilbert (1995) ArticleTitleIntron phase correlations and the evolution of the intron/exon structure of genes. Proc Natl Acad Sci USA 92 12495–12499 Occurrence Handle1:CAS:528:DyaK28Xhtl2rtw%3D%3D Occurrence Handle8618928
Oppentocht J (2001) Alcohol dehydrogenase and alcohol tolerance in Drosophila. Thesis, University of Groningen, 133 pp
M Pelandakis DG Higgins M Solignac (1991) ArticleTitleMolecular phylogeny of the subgenus Sophophora of Drosophila derived from large subunit of ribosomal RNA sequences. Genetica 84 87–94 Occurrence Handle1:CAS:528:DyaK38XhtVaqtbw%3D Occurrence Handle1756966
M Pelandakis M Solignac (1993) ArticleTitleMolecular phylogeny of Drosophila based on ribosomal RNA sequences. J Mol Evol 37 525–543 Occurrence Handle1:CAS:528:DyaK2cXisVSltbg%3D Occurrence Handle8283482
T Schafer M Selig P Schoneit (1993) ArticleTitleAcetyl-CoA synthetase (ADP forming) in archaea, a novel enzyme involved in acetate formation. Arch Microbiol 159 72–83
A Stoltzfus JM Logsdon JD Palmer WF Doolittle (1997) ArticleTitleIntron ‘‘sliding’’ and the diversity of intron positions. Proc Natl Acad Sci USA 94 10739–10744 Occurrence Handle10.1073/pnas.94.20.10739 Occurrence Handle1:CAS:528:DyaK2sXmtlekt7g%3D Occurrence Handle9380704
DL Swofford (2000) Phylogenetic analysis using parsimony. Version 4.0b4a. Smithsonian Institution Washington, DC
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Karan, D., Lesbats, M., David, J.R. et al. Evolution of the AMP-forming Acetyl-CoA Synthetase Gene in the Drosophilidae Family . J Mol Evol 57 (Suppl 1), S297–S303 (2003). https://doi.org/10.1007/s00239-003-0040-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s00239-003-0040-1