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Hyaluronidases for treating complications by hyaluronic acid dermal fillers: evaluation of the effects on cell cultures and human skin

  • Experimental Study
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European Journal of Plastic Surgery Aims and scope Submit manuscript

Abstract

Background

Hyaluronidases are commonly employed in aesthetic medicine to reverse the effects of the injected hyaluronic acid filler, eliminating nodules, correcting the injection of excessive quantities of filler, or reducing the odds of ischemic complications, although toxicity studies and clear guidelines are lacking in the literature. The objective of this study was to evaluate effects of hyaluronidases on skin and soft tissue viability.

Methods

Human fibroblast cell and human skin cultures were grown under different concentrations of hyaluronidases. Cell viability and proliferation were tested with the hemocytometric assay with Trypan blue and colorimetric assay with 4,5-dimethylthiazol-22,5-difenil tetrazolium bromide.

Results

The viability of fibroblast cultures was not significantly affected by the enzyme concentration or the time of exposure, exceeding 70 % of viability in all cases. The viability of the tissue samples showed a decrease, inferior to 70 % of viability at 6.5 and 14 U, independently from the time of exposure, although not statistically significant.

Conclusions

Hyaluronidases do not affect fibroblast proliferation, although high concentrations of the enzyme may be able to inhibit human skin viability. This result could be influenced by the absence of growth medium; this result should be confirmed by further studies.

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Conflict of Interest

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Authors and Affiliations

  1. Servizio di Chirurgia Plastica, Centro Diagnostico Italiano, Università degli Studi di Milano, Milan, Italy

    M. Cavallini

  2. Plastic Surgery Department, Università degli Studi di Milano, IRCCS Policlinico San Donato, Piazza Malan, San Donato Milanese, 20097, Milan, Italy

    R. Gazzola

  3. S.S. Terapia Tissutale–Centro di Riferimento Regionale per la Coltura di Epidermide Umana in Vitro e banca per la Crioconservazione dei Tessuti A.O. Ospedale Niguarda Ca’ Granda, Milan, Italy

    B. Antonioli, M. Tosca, M. Galuzzi & M. Marazzi

  4. Dipartimento Emergenza e Accettazione–E.A.S. S.C: Chirurgia Plastica e Centro Grandi Ustionati A.O. Ospedale Niguarda Ca’ Granda, Milan, Italy

    V. Rapisarda & F. Ciancio

Authors
  1. M. Cavallini
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  2. B. Antonioli
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  3. R. Gazzola
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  4. M. Tosca
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  5. M. Galuzzi
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  6. V. Rapisarda
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  8. M. Marazzi
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Correspondence to R. Gazzola.

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Cavallini, M., Antonioli, B., Gazzola, R. et al. Hyaluronidases for treating complications by hyaluronic acid dermal fillers: evaluation of the effects on cell cultures and human skin. Eur J Plast Surg 36, 477–484 (2013). https://doi.org/10.1007/s00238-013-0855-y

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  • DOI: https://doi.org/10.1007/s00238-013-0855-y

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