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The effect of the atelocollagen matrix and bFGF on bone regeneration in defects of the calvaria of rats

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Abstract

The purpose of this study was to evaluate the effect of the atelocollagen matrix and basic fibroblast growth factor (bFGF) on bone regeneration in a bone defect of the calvaria. The subjects were 10-week-old male Wister rats weighing 350 g. Two kinds of atelocollagen matrices, which were either strongly cross-linked or weakly cross-linked, were placed in defects created in the rat cranium. Two doses of bFGF, either 30 μg or 50 μg, were injected into the weakly cross-linked atelocollagen matrix. Both the bone mineral content in the defects and the area of bone regeneration in the median sections of the defects were measured 2, 4, and 8 weeks after surgical treatment. Our findings suggested that the weakly cross-linked atelocollagen matrix tends to demonstrate a scaffold of bone regeneration, and an appropriate dosage of bFGF may inhibit bone absorption to maintain the volume of the regenerated bone.

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Acknowledgements

We express our thanks to Shunichi Yamashita, M.D., the professor of Molecular Medicine and International Radiation Health at the Institute for Atomic Bomb Sequelae Medicine of Nagasaki University Graduate School of Biomedical Science, who provided very helpful advice in this study. We also express our thanks to Mr. Masanobu Anami, the chief engineer of the Department of Clinical Examination in the Nagasaki University Hospital, who kindly made the specimen sections.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Nagasaki Yuai Hospital, 2314-1 Kayaki-machi, Nagasaki, 851-0401, Japan

    M. Nakamura

  2. Department of Plastic and Reconstructive Surgery, Ehime Prefectural Hospital, Matsuyama, Japan

    K. Kobayashi

  3. Division of Plastic and Reconstructive Surgery, Nagasaki University Graduate School of Biomedical Science, Nagasaki, Japan

    A. Hirano

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  1. M. Nakamura
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  2. K. Kobayashi
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  3. A. Hirano
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Correspondence to M. Nakamura.

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Nakamura, M., Kobayashi, K. & Hirano, A. The effect of the atelocollagen matrix and bFGF on bone regeneration in defects of the calvaria of rats. Eur J Plast Surg 28, 517–522 (2006). https://doi.org/10.1007/s00238-006-0038-1

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  • DOI: https://doi.org/10.1007/s00238-006-0038-1

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