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Anticancer Activity Studies of Ruthenium(II) Complex Toward Human Osteosarcoma HOS Cells

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Abstract

A new Ru(II) complex [Ru(dmp)2(NMIP)](ClO4)2 (dmp = 2,9-dimethyl-1,10-phenanthroline, NMIP = 2’-(2″-nitro-3″,4″-methylenedioxyphenyl)imidazo[4′,5′-f][1,10]-phenanthroline) was synthesized and characterized by elemental analysis, ESI–MS and 1H NMR. The cytotoxic activity of the complex against MG-63, U2OS, HOS, and MC3T3-e1 cell lines was investigated by MTT method. The complex shows moderate cytotoxicity toward HOS (IC50 = 35.6 ± 2.6 µM) and MC3T3-e1 (IC50 = 41.6 ± 2.8 µM) cell lines. The morphological studies show that the complex can induce apoptosis in HOS cells and cause an increase of reactive oxygen species levels and a decrease in the mitochondrial membrane potential. The cell cycle distribution demonstrates that the complex inhibits the cell growth at S phase. Additionally, the antitumor activity in vivo reveals that the complex can induce a decrease in tumor weight.

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Acknowledgments

This work was supported by the National Nature Science Foundation of China (Nos. 81272941, 81201864), the Health and Family Planning Commission of Guangzhou Municipality (20141A010001), and the Guangdong Provincial Department of Science and Technology (2014A020212009).

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    Authors and Affiliations

    1. Department of Orthopaedics, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou, 510180, People’s Republic of China

      Jian-Wei Zhu, Si-Hong Liu, Gui-Qiang Zhang, Hui-Hua Xu, Yu-Xuan Wang, Ya-Min Liu, Yan Wang, Jun-Bo Liang & Qi-Feng Guo

    2. Department of Oncology, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou, 510180, People’s Republic of China

      Yong Wu

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    1. Jian-Wei Zhu
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    Correspondence to Qi-Feng Guo.

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    Jian-Wei Zhu and Si-Hong Liu contributed equally to this work.

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    Zhu, JW., Liu, SH., Zhang, GQ. et al. Anticancer Activity Studies of Ruthenium(II) Complex Toward Human Osteosarcoma HOS Cells. J Membrane Biol 249, 483–492 (2016). https://doi.org/10.1007/s00232-016-9889-y

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    • DOI: https://doi.org/10.1007/s00232-016-9889-y

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