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Morphological Changes of Proteolipid Giant Unilamellar Vesicles Affected by Macrovipera lebetina obtusa Venom Visualized with Fluorescence Microscope

Abstract

As a rule, zootoxins are complex and biologically active, and therefore the greater part of zootoxins is subjected to biotransformation and interacts with biological membranes. In this case, the interaction of different venom components with the membranes is not always the same. The present study shows how the giant unilamellar vesicles (GUV) from bovine brain proteolipids interact with Macrovipera lebetina obtusa venom. GUV (mean diameter 30 μm) were formed by the electroformation method. We used 8-anilino-1-naphthalenesulfonic acid and pyrene as fluorescence probes, which allowed us to quantify the fluidity changes in the membrane by measuring the fluorescence intensity.

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Acknowledgments

This work was supported by grant N11-1f231 from the State Committee of Science, Ministry of Education and Science RA.

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The authors report that they have no conflicts of interest.

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Correspondence to N. A. Ghazaryan.

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Ghazaryan, N.A., Ghulikyan, L.A. & Ayvazyan, N.M. Morphological Changes of Proteolipid Giant Unilamellar Vesicles Affected by Macrovipera lebetina obtusa Venom Visualized with Fluorescence Microscope. J Membrane Biol 246, 627–632 (2013). https://doi.org/10.1007/s00232-013-9576-1

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  • DOI: https://doi.org/10.1007/s00232-013-9576-1

Keywords

  • Proteolipids
  • GUV
  • Macrovipera lebetina obtusa
  • ANS
  • Pyrene