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Adhesion Signals of Phospholipid Vesicles at an Electrified Interface

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Abstract

General adhesion behavior of phospholipid vesicles was examined in a wide range of potentials at the mercury electrode by recording time-resolved adhesion signals. It was demonstrated that adhesion-based detection is sensitive to polar headgroups in phospholipid vesicles. We identified a narrow potential window around the point of zero charge of the electrode where the interaction of polar headgroups of phosphatidylcholine vesicles with the substrate is manifested in the form of bidirectional signals. The bidirectional signal is composed of the charge flow due to the nonspecific interaction of vesicle adhesion and spreading and of the charge flow due to a specific interaction of the negatively charged electrode and the most exposed positively charged choline headgroups. These signals are expected to appear only when the electrode surface charge density is less than the surface charge density of the choline groups at the contact interface. In comparison, for the negatively charged phosphatidylserine vesicles, we identified the potential window at the mercury electrode where charge compensation takes place, and bidirectional signals were not detected.

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Acknowledgements

This study was supported by the Croatian Ministry of Science, Education and Sports, projects 098-0982934-2744 and 021-0212432-2431. Special thanks are due to Ivica Ružić for help with charge density calculations.

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Authors and Affiliations

  1. Ruđer Bošković Institute, P.O. Box 180, 10002, Zagreb, Croatia

    Nadica Ivošević DeNardis, Vera Žutić & Vesna Svetličić

  2. Institute of Immunology, P.O. Box 266, 10000, Zagreb, Croatia

    Ruža Frkanec

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  1. Nadica Ivošević DeNardis
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  2. Vera Žutić
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  3. Vesna Svetličić
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  4. Ruža Frkanec
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Correspondence to Nadica Ivošević DeNardis.

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DeNardis, N.I., Žutić, V., Svetličić, V. et al. Adhesion Signals of Phospholipid Vesicles at an Electrified Interface. J Membrane Biol 245, 573–582 (2012). https://doi.org/10.1007/s00232-012-9469-8

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  • DOI: https://doi.org/10.1007/s00232-012-9469-8

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