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First-Leaflet Phase Effect on Properties of Phospholipid Bilayer Formed Through Vesicle Adsorption on LB Monolayer

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Abstract

Phospholipid bilayers were formed on mica using the Langmuir–Blodgett technique and liposome fusion, as a model system for biomembranes. Nanometer-scale surface physical properties of the bilayers were quantitatively characterized upon the different phases of the first leaflets. Lower hydration/steric forces on the bilayers were observed at the liquid phase of the first leaflet than at the solid phase. The forces appear to be related to the low mechanical stability of the lipid bilayer, which was affected by the first leaflet phase. The first leaflet phase also influenced the long-range repulsive forces over the second leaflet. Surface forces, measured using a modified probe with an atomic force microscope, showed that lower long-range repulsive forces were also found at the liquid phase of the first leaflet. Force measurements were performed at 300 mM sodium chloride solution so that the effect of the phase on the long-range repulsive forces could be investigated by reducing the effect of the repulsion between the second-leaflet lipid headgroups on the long-range repulsive forces. Forces were analyzed using the Derjaguin–Landau–Verwey–Overbeek theory so that the surface potential and surface charge density of the lipid bilayers were quantitatively acquired for each phase of the first leaflet.

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Acknowledgments

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0010097). We thank all of the members of the Department of Chemical Engineering, Seoul National University of Technology, for help and valuable discussions. We thank Prof. I. S. Yoo at Kyungwon University and Prof. D. J. Ahn, Dr. G. S. Lee, Mr. H. Choi and Mr. C. S. Choi at Korea University for valuable help.

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  1. Department of Chemical Engineering, School of Engineering, Seoul National University of Science and Technology, 172 Gongreung2dong, Nowonku, Seoul, 173-743, South Korea

    Jin-Won Park

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Correspondence to Jin-Won Park.

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Park, JW. First-Leaflet Phase Effect on Properties of Phospholipid Bilayer Formed Through Vesicle Adsorption on LB Monolayer. J Membrane Biol 237, 107–114 (2010). https://doi.org/10.1007/s00232-010-9311-0

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