Journal of Membrane Biology

, Volume 233, Issue 1–3, pp 135–142 | Cite as

Melatonin Modulates Hippocampus NMDA Receptors, Blood and Brain Oxidative Stress Levels in Ovariectomized Rats

  • Müfide Dilek
  • Mustafa Nazıroğlu
  • H. Baha Oral
  • İ. Suat Övey
  • Mustafa Küçükayaz
  • M. Tamer Mungan
  • H. Yusuf Kara
  • Recep Sütçü


We investigated the effects of melatonin administration on ovariectomy-induced oxidative toxicity and N-methyl-d-aspartate receptor (NMDAR) subunits in the blood of rats. Thirty-two rats were studied in three groups. The first and second groups were control and ovariectomized rats. Melatonin was daily administrated to the ovariectomized rats in the third group for 30 days. Blood, brain cortical and hippocampal samples were taken from the three groups after 30 days. Brain cortical, erythrocyte and plasma lipid peroxidation (LP) levels were higher in the ovariectomized group than in controls, although the LP level was decreased in the ovariectomized group with melatonin treatment. Brain cortical and plasma concentrations of vitamins A, C and E as well as the NMDAR 2B subunit were lower in the ovariectomized group than in controls, although, except for plasma vitamin C, they were increased by the treatment. Brain cortical and erythrocyte reduced glutathione (GSH) levels were lower in the ovariectomized group than in controls, although erythrocyte GSH levels were higher in the melatonin group than in the ovariectomized group. Brain cortical and erythrocyte glutathione peroxidase activity and NMDAR 2A subunit concentrations were not found to be different in all groups statistically. Oxidative stress has been proposed to explain the biological side effect of experimental menopause. Melatonin prevents experimental menopause–induced oxidative stress to strengthen antioxidant vitamin and NMDAR 2A subunit concentrations in ovariectomized rats.


Oxidative stress Melatonin Hippocampal NMDA receptor Brain Reproduction 



B. O. and M. N. formulated the present hypothesis and were responsible for writing the report. M. D., I. S. O., H. Y. K. and R. S. were responsible for data analyses. M. T. M. made critical revisions to the manuscript. The study was partially supported by the Scientific Research Project Unit of Suleyman Demirel University (BAP-2008).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Müfide Dilek
    • 1
  • Mustafa Nazıroğlu
    • 2
    • 3
  • H. Baha Oral
    • 1
  • İ. Suat Övey
    • 2
  • Mustafa Küçükayaz
    • 2
  • M. Tamer Mungan
    • 1
  • H. Yusuf Kara
    • 4
  • Recep Sütçü
    • 4
  1. 1.Department of Gynecology, Medical FacultySuleyman Demirel UniversityIspartaTurkey
  2. 2.Department of Biophysics, Medical FacultySuleyman Demirel UniversityIspartaTurkey
  3. 3.Neuroscience Research CenterSuleyman Demirel UniversityIspartaTurkey
  4. 4.Department of Biochemistry, Medical FacultySuleyman Demirel UniversityIspartaTurkey

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