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DNA-Induced Aggregation and Fusion of Phosphatidylcholine Liposomes in the Presence of Multivalent Cations Observed by the Cryo-TEM Technique

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Abstract

By means of cryoelectron transmission microscopy (cryo-TEM), we were able to demonstrate the formation of ternary complexes (TC): DNA–phosphatidylcholine liposome–divalent metal cations. Addition of Ba2+ to TC led to visualization of DNA compacting on the liposome surface. Staining the TC by Tb3+ cations revealed the changed secondary structure of DNA located between fused liposomes. Cryo-TEM and liposome turbidity data were analyzed during TC formation. Liposome aggregation and the liposome fusion induced by DNA in TC were observed. Because TC displayed the property of DNA cationic liposome complexes as well as their own unique properties, we were able to consider cationic lipoplexes as a particular case of TC. The involvement of TC and direct DNA–lipid interactions in the formation nuclear pore complexes were assumed.

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Authors and Affiliations

  1. Department of Mechanisms Reception of the Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Reg., Russia

    Vasily V. Kuvichkin

  2. Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 5-1, Higashiyama, Myodaiji-cho, Okazaki, 444-8787, Japan

    Radostin S. Danev, Hideki Shigematsu & Kuniaki Nagayama

Authors
  1. Vasily V. Kuvichkin
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  2. Radostin S. Danev
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  3. Hideki Shigematsu
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  4. Kuniaki Nagayama
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Correspondence to Vasily V. Kuvichkin.

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Kuvichkin, V.V., Danev, R.S., Shigematsu, H. et al. DNA-Induced Aggregation and Fusion of Phosphatidylcholine Liposomes in the Presence of Multivalent Cations Observed by the Cryo-TEM Technique. J Membrane Biol 227, 95–103 (2009). https://doi.org/10.1007/s00232-008-9146-0

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  • DOI: https://doi.org/10.1007/s00232-008-9146-0

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