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Association of Renal Na,K-ATPase α-Subunit with the β- and γ-Subunits Based on Cryoelectron Microscopy

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Abstract

Na,K-ATPase transports Na+ and K+ across cell membranes and consists of α- and β-subunits. Na,K-ATPase also associates with small FXYD proteins that regulate the activity of the pump. We have used cryoelectron microscopy of two-dimensional crystals including data to 8 Å resolution to determine the three-dimensional (3-D) structure of renal Na,K-ATPase containing FXYD2, the γ-subunit. A homology model for the α-subunit was calculated from a Ca2+-ATPase structure and used to locate the additional β- and γ-subunits present in the 3-D map of Na,K-ATPase. Based on the 3-D map, the β-subunit is located close to transmembrane helices M8 and M10 and the γ-subunit is adjacent to helices M2 and M9 of the α-subunit.

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Acknowledgment

This work was supported by grants from the Swedish Research Council, the Danish Medical Research Council and the Water and Salt Research Center (established and supported by the Danish National Research Foundation, Grundforskningsfonden). P. P. acknowledges a fellowship from the Emil Aaltonen Foundation.

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Authors and Affiliations

  1. Department of Biosciences and Nutrition and School of Technology and Health, Karolinska Institutet, Royal Institute of Technology, S-141 57, Huddinge, Sweden

    P. Purhonen & H. Hebert

  2. Institute of Medical Technology, University of Tampere, FI-33014, Tampere, Finland

    P. Purhonen

  3. Water and Salt Research Center, Department of Cell Biology, Institute of Anatomy, University of Aarhus, DK-8000, Aarhus, Denmark

    K. Thomsen & A.B. Maunsbach

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  1. P. Purhonen
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  2. K. Thomsen
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  4. H. Hebert
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Correspondence to H. Hebert.

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Purhonen, P., Thomsen, K., Maunsbach, A. et al. Association of Renal Na,K-ATPase α-Subunit with the β- and γ-Subunits Based on Cryoelectron Microscopy. J Membrane Biol 214, 139–146 (2006). https://doi.org/10.1007/s00232-006-0056-8

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  • DOI: https://doi.org/10.1007/s00232-006-0056-8

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