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Effects of Temperature on Viral Glycoprotein Mobility and a Possible Role of Internal “Viroskeleton” Proteins in Sendai Virus Fusion

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Abstract

The effect of temperature on fusion of Sendai virus with target membranes and mobility of the viral glycoproteins was studied with fluorescence methods. When intact virus was used, the fusion threshold temperature (20–22°C) was not altered regardless of the different types of target membranes. Viral glycoprotein mobility in the intact virus increased with temperature, particularly sharply at the fusion threshold temperature. This effect was suppressed by the presence of erythrocyte ghosts and/or dextran sulfate in the virus suspension. In these cases also, no change in the fusion threshold temperature was observed. On the other hand, reconstituted viral envelopes (virosomes) bearing viral glycoproteins but lacking matrix proteins were capable of fusing with erythrocyte ghosts even at temperatures lower than the fusion threshold temperature and no fusion threshold temperature was observed over the range of 10–40°C. The mobility of viral glycoproteins on virosomes was much greater and virtually temperature-independent. The intact virus treated with an actin-affector, jasplakinolide, reduced the extent of fusion with erythrocyte ghosts and the mobility of viral glycoproteins, while the treatment of virosomes with the same drug did not affect the extent of fusion of virosomes with erythrocyte ghosts and the mobility of the glycoproteins. These results suggest that viral matrix proteins including actins affect viral glycoprotein mobility and may be responsible for the temperature threshold phenomenon observed in Sendai virus fusion.

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Authors and Affiliations

  1. Department of Physiology & Biophysics, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY, 14214, USA

    S. Ohki

  2. Department of Microbiology & Immunology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY, 14214, USA

    H. Thacore & T. D. Flanagan

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  1. S. Ohki
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Ohki, S., Thacore, H. & Flanagan, T.D. Effects of Temperature on Viral Glycoprotein Mobility and a Possible Role of Internal “Viroskeleton” Proteins in Sendai Virus Fusion. J Membrane Biol 199, 73–83 (2004). https://doi.org/10.1007/s00232-004-0678-7

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