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Chemical and Photochemical Modification of Colicin E1 and Gramicidin A in Bilayer Lipid Membranes

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Abstract

Chemical modification and photodynamic treatment of the colicin E1 channel-forming domain (P178) in vesicular and planar bilayer lipid membranes (BLMs) was used to elucidate the role of tryptophan residues in colicin E1 channel activity. Modification of colicin tryptophan residues by N-bromosuccinimide (NBS), as judged by the loss of tryptophan fluorescence, resulted in complete suppression of wild-type P178 channel activity in BLMs formed from fully saturated (diphytanoyl) phospholipids, both at the macroscopic-current and single-channel levels. The similar effect on both the tryptophan fluorescence and the electric current across BLM was observed also after NBS treatment of gramicidin channels. Of the single-tryptophan P178 mutants studied, W460 showed the highest sensitivity to NBS treatment, pointing to the importance of the water-exposed Trp460 in colicin channel activity. In line with previous work, the photodynamic treatment (illumination with visible light in the presence of a photosensitizer) led to suppression of P178 channel activity in diphytanoyl-phospholipid membranes concomitant with the damage to tryptophan residues detected here by a decrease in tryptophan fluorescence. The present work revealed novel effects: activation of P178 channels as a result of both NBS and photodynamic treatments was observed with BLMs formed from unsaturated (dioleoyl) phospholipids. These phenomena are ascribed to the effect of oxidative modification of double-bond-containing lipids on P178 channel formation. The pronounced stimulation of the colicin-mediated ionic current observed after both pretreatment with NBS and sensitized photomodification of the BLMs support the idea that distortion of membrane structure can facilitate channel formation.

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References

  • M.D. Becker D.V. Greathouse R.E. Koeppe O.S. Andersen (1991) ArticleTitleAmino acid sequence modulation of gramicidin channel function: effects of tryptophan-to-phenylalanine substitutions on the single-channel conductance and duration Biochemistry 30 8830–8839 Occurrence Handle10.1021/bi00100a015 Occurrence Handle1716152

    Article  PubMed  Google Scholar 

  • S. Bhakdi H. Bayley A. Valeva I. Walev B. Walker M. Kehoe M. Palmer (1996) ArticleTitleStaphylococcal alpha-toxin, streptolysin-O, and Escherichia coli hemolysin: prototypes of pore-forming bacterial cytolysins Arch. Microbiol 165 73–79 Occurrence Handle10.1007/s002030050300 Occurrence Handle8593102

    Article  PubMed  Google Scholar 

  • L.J Bishop E.S. Bjes V.L Davidson W.A Cramer (1985) ArticleTitleLocalization of the immunity protein-reactive domain in unmodified and chemically modified COOH-terminal peptides of colicin E1 J. Bacteriol 164 237–244 Occurrence Handle4044520

    PubMed  Google Scholar 

  • L.J. Bishop F.S. Cohen V.L. Davidson W.A. Cramer (1986) ArticleTitleChemical modification of the two histidine and single cysteine residues in the channel-forming domain of colicin E1. J Membrane Biol 92 237–245 Occurrence Handle10.1007/BF01869392

    Article  Google Scholar 

  • L.T. Boni A.J. Connolly A.M. Kleinfeld (1986) ArticleTitleTransmembrane distribution of gramicidin by tryptophan energy transfer Biophys, J 49 122–123

    Google Scholar 

  • J.O. Bullock (1992) ArticleTitleIon selectivity of colicin EI: modulation by pH and membrane composition J. Membrane Biol 125 255–271 Occurrence Handle10.1007/BF00236438

    Article  Google Scholar 

  • J.O. Bullock F.S. Cohen (1986) ArticleTitleOctyl glucoside promotes incorporation of channels into neutral planar phospholipid bilayers: studies with colicin Ia Biochim. Biophys. Acta 856 101–108 Occurrence Handle2420358

    PubMed  Google Scholar 

  • D.D. Busath (1993) ArticleTitleThe use of physical methods in determining gramicidin channel structure and function Annu. Rev. Physiol 55 473–501 Occurrence Handle10.1146/annurev.ph.55.030193.002353 Occurrence Handle7682046

    Article  PubMed  Google Scholar 

  • D.D. Busath R.C. Waldbillig (1983) ArticleTitlePhotolysis of gramicidin A channels in lipid bilayers Biochim. Biophys. Acta 736 28–38

    Google Scholar 

  • A.M. Campos E.A. Lissi C. Vergara M.E. Lanio C. Alvarez I. Pazos V. Morera Y. Garcia D. Martinez (1999) ArticleTitleKinetics and mechanism of St I modification by peroxyl radicals J. Protein Chem 18 297–306 Occurrence Handle10.1023/A:1021087312176 Occurrence Handle10395448

    Article  PubMed  Google Scholar 

  • A.C. Carr C.C. Winterbourn J.J. van den Berg (1996) ArticleTitlePeroxidase-mediated bromination of unsaturated fatty acids to form bromohydrins Arch. Biochem. Biophys 327 227–233 Occurrence Handle10.1006/abbi.1996.0114 Occurrence Handle8619607

    Article  PubMed  Google Scholar 

  • M.V. Cleveland S. Slatin A. Finkelstein C. Levinthal (1983) ArticleTitleStructure-function relationships for a voltage-dependent ion channel: properties of COOH-terminal fragments of colicin E1 Proc. Natl. Acad. Sci. USA 80 3706–3710 Occurrence Handle6304732

    PubMed  Google Scholar 

  • E.C. Cooper S.A. Tomiko W.S. Agnew (1987) ArticleTitleReconstituted voltage-sensitive sodium channel from Electrophorus electricus: chemical modifications that alter regulation of ion permeability Proc. Natl. Acad. Sci. USA 84 6282–6286 Occurrence Handle2442755

    PubMed  Google Scholar 

  • S.W. Cowan T. Schirmer G. Rummel M. Steiert R. Ghosh R.A. Pauptit J.N. Jansonius J.P. Rosenbusch (1992) ArticleTitleCrystal structures explain functional properties of two E.coli porins Nature 358 727–733 Occurrence Handle10.1038/358727a0 Occurrence Handle1380671

    Article  PubMed  Google Scholar 

  • W.A. Cramer J.B. Heymann S.L. Schendel B.N. Deriy F.S. Cohen P.A. Elkins C.V. Stauffacher (1995) ArticleTitleStructure-function of the channel-forming colicins Annu. Rev. Biophys. Biomol. Struct 24 611–641 Occurrence Handle10.1146/annurev.bb.24.060195.003143 Occurrence Handle7545041

    Article  PubMed  Google Scholar 

  • J. Deisenhofer H. Michel (1989) ArticleTitleNobel lecture. The photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis EMBO J 8 2149–2170 Occurrence Handle2676514

    PubMed  Google Scholar 

  • B. Ehrenberg J.L. Anderson C.S. Foote (1998) ArticleTitleKinetics and yield of singlet oxygen photosensitized by hypericin in organic and biological media Photochem. Photobiol 68 135–140 Occurrence Handle10.1562/0031-8655(1998)068<0135:KAYOSO>2.3.CO;2 Occurrence Handle9723207

    Article  PubMed  Google Scholar 

  • P. Elkins A. Bunker W.A. Cramer C.V. Stauffacher (1997) ArticleTitleA mechanism for toxin insertion into membranes is suggested by the crystal structure of the channel-forming domain of colicin E1 Structure 5 443–458 Occurrence Handle10.1016/S0969-2126(97)00200-1 Occurrence Handle9083117

    Article  PubMed  Google Scholar 

  • R.M. Epand H.J. Vogel (1999) ArticleTitleDiversity of antimicrobial peptides and their mechanisms of action Biochim. Biophys. Acta 1462 11–28 Occurrence Handle10590300

    PubMed  Google Scholar 

  • J.L. Galzi D. Bertrand A. Devillers-Thiery F. Revah S. Bertrand J.P. Changeux (1991) ArticleTitleFunctional significance of aromatic amino acids from three peptide loops of the alpha 7 neuronal nicotinic receptor site investigated by site-directed mutagenesis FEBS Lett 294 198–202 Occurrence Handle10.1016/0014-5793(91)80668-S Occurrence Handle1756861

    Article  PubMed  Google Scholar 

  • E. Gouaux (1997) ArticleTitlelong and short of colicin action: the molecular basis for the biological activity of channel-forming colicins Structure 5 313–317 Occurrence Handle10.1016/S0969-2126(97)00188-3 Occurrence Handle9083116

    Article  PubMed  Google Scholar 

  • W. Hu T.A. Cross (1995) ArticleTitleTryptophan hydrogen bonding and electric dipole moments: functional roles in the gramicidin channel and implications for membrane proteins Biochemistry 34 14147–14155 Occurrence Handle10.1021/bi00043a020 Occurrence Handle7578012

    Article  PubMed  Google Scholar 

  • K.S. Jakes P.K. Kienker A. Finkelstein (1999) ArticleTitleChannel-forming colicins: translocation (and other deviant behaviour) associated with colicin Ia channel gating Q. Rev. Biophys 32 189–205 Occurrence Handle10.1017/S0033583599003492 Occurrence Handle10845238

    Article  PubMed  Google Scholar 

  • D. Jones E. Hayon D. Busath (1986) ArticleTitleTryptophan photolysis is responsible for gramicidin-channel inactivation by ultraviolet light Biochim. Biophys. Acta 861 62–66 Occurrence Handle2428397

    PubMed  Google Scholar 

  • J.A. Killian (1992) ArticleTitleGramicidin and gramicidin-lipid interactions Biochim. Biophys. Acta 1113 391–425 Occurrence Handle1280467

    PubMed  Google Scholar 

  • F. Killig G. Stark (2002) ArticleTitlePhotodynamic activation of ion transport through lipid membranes and its correlation with an increased dielectric constant of the membrane Biochim. Biophys. Acta 1564 207–213 Occurrence Handle12101014

    PubMed  Google Scholar 

  • R.E. Koeppe O.S. Andersen (1996) ArticleTitleEngineering the gramicidin channel Annu. Rev. Biophys. Biomol. Struct 25 231–258 Occurrence Handle8800470

    PubMed  Google Scholar 

  • Y.E. Korchev C.L. Bashford C. Pederzolli C.A. Pasternak P.J. Morgan P.W. Andrew T.J. Mitchell (1998) ArticleTitleA conserved tryptophan in pneumolysin is a determinant of the characteristics of channels formed by pneumolysin in cells and planar lipid bilayers Biochem. J 329 571–577 Occurrence Handle9445384

    PubMed  Google Scholar 

  • A.A., Krasnovsky SuffixJr. (1998) ArticleTitleSinglet molecular oxygen in photobiochemical systems: IR phosphorescence studies Membr. Cell Biol 12 665–690 Occurrence Handle10379647

    PubMed  Google Scholar 

  • A. Kreusch A. Neubuser E. Schiltz J. Weckesser G.E. Schulz (1994) ArticleTitleStructure of the membrane channel porin from Rhodopseudomonas blastica at 2.0 Å resolution Protein Sci 3 58–63 Occurrence Handle8142898

    PubMed  Google Scholar 

  • L. Kunz U. Zeidler K. Haegele M. Przybylski G. Stark (1995) ArticleTitlePhotodynamic and radiolytic inactivation of ion channels formed by gramicidin A: oxidation and fragmentation Biochemistry 34 11895–11903 Occurrence Handle10.1021/bi00037a030 Occurrence Handle7547925

    Article  PubMed  Google Scholar 

  • J.H. Lakey D. Baty F. Pattus (1991) ArticleTitleFluorescence energy transfer distance measurements using site-directed single cysteine mutants. The membrane insertion of colicin A J. Mol. Biol 218 639–653 Occurrence Handle10.1016/0022-2836(91)90707-D Occurrence Handle2016750

    Article  PubMed  Google Scholar 

  • J.H. Lakey S.L. Slatin (2001) ArticleTitlePore-forming colicins and their relatives Curr. Top. Microbiol. Immunol 257 131–161 Occurrence Handle11417119

    PubMed  Google Scholar 

  • A. Lavi H. Weitman R.T. Holmes K.M. Smith B. Ehrenberg (2002) ArticleTitleThe depth of porphyrin in a membrane and the membrane’s physical properties affect the photosensitizing efficiency Biophys. J 82 2101–2110 Occurrence Handle11916866

    PubMed  Google Scholar 

  • C.J. Lazdunski D. Baty V. Geli D. Cavard J. Morion R. Lloubes S.P. Howard M. Knibiehler M. Chartier S. Varenne (1988) ArticleTitleThe membrane channel-forming colicin A: synthesis, secretion, structure, action and immunity Biochim. Biophys. Acta 947 445–464 Occurrence Handle3139035

    PubMed  Google Scholar 

  • M. Lindeberg S.D. Zakharov W.A. Cramer (2000) ArticleTitleUnfolding pathway of the colicin E1 channel protein on a membrane surface J. Mol. Biol 295 679–692 Occurrence Handle10.1006/jmbi.1999.3396 Occurrence Handle10623556

    Article  PubMed  Google Scholar 

  • B.A. Lindig M.A.J. Rodgers (1981) ArticleTitleRate parameters for the quenching of singlet oxygen by water-soluble and lipid-soluble substrates in aqueous and micellar systems Photochem. Photobiol 33 627–634

    Google Scholar 

  • R.L Lundblad C.M Noyes (1985) Chemical modification of tryptophan Chemical Reagents for Protein Modification CRC Press Boca Raton, Florida 47–71

    Google Scholar 

  • S.E. Malenbaum A.R. Merrill E. London (1998) ArticleTitleMembrane-inserted colicin E1 channel domain: a topological survey by fluorescence quenching suggests that model membrane thickness affects membrane penetration J. Nat. Toxins 7 269–290 Occurrence Handle9783264

    PubMed  Google Scholar 

  • P. Malovrh A. Barlic Z. Podlesek P. Macek G. Menestrina G. Anderluh (2000) ArticleTitleStructure-function studies of tryptophan mutants of equinatoxin II, a sea anemone pore-forming protein Biochem. J 346 223–232 Occurrence Handle10.1042/0264-6021:3460223 Occurrence Handle10657261

    Article  PubMed  Google Scholar 

  • A.R. Merrill W.A. Cramer (1990) ArticleTitleIdentification of a voltage-responsive segment of the potential-gated colicin E1 ion channel Biochemistry 29 8529–8534 Occurrence Handle10.1021/bi00489a004 Occurrence Handle1702993

    Article  PubMed  Google Scholar 

  • M. Montoya E. Gouaux (2003) ArticleTitleBeta-barrel membrane protein folding and structure viewed through the lens of alpha-hemolysin Biochim. Biophys. Acta 1609 19–27 Occurrence Handle12507754

    PubMed  Google Scholar 

  • P. Mueller D.O. Rudin H.T. Tien W.C. Wescott (1963) ArticleTitleMethods for the formation of single bimolecular lipid membranes in aqueous solution J. Phys. Chem 67 534–535

    Google Scholar 

  • G.S. Oxford C.H. Wu T. Narahashi (1978) ArticleTitleRemoval of sodium channel inactivation in squid giant axons by N-bromoacetamide J. Gen. Physiol 71 227–247 Occurrence Handle10.1085/jgp.71.3.227 Occurrence Handle650167

    Article  PubMed  Google Scholar 

  • L.R. Palmer A.R. Merrill (1994) ArticleTitleMapping the membrane topology of the closed state of the colicin E1 channel J. Biol. Chem 269 4187–4193 Occurrence Handle7508440

    PubMed  Google Scholar 

  • A. Pautsch G.E. Schulz (1998) ArticleTitleStructure of the outer membrane protein A transmembrane domain Nat. Struct. Biol 5 1013–1017 Occurrence Handle10.1038/2983 Occurrence Handle9808047

    Article  PubMed  Google Scholar 

  • B.F. Peterman K.J. Laidler (1979) ArticleTitleThe reactivity of tryptophan residues in proteins: stopped-flow kinetics of fluorescence quenching Biochim. Biophys. Acta 577 314–323 Occurrence Handle454650

    PubMed  Google Scholar 

  • J.L. Popot D.M. Engelman (2000) ArticleTitleHelical membrane protein folding, stability, and evolution Annu. Rev. Biochem 69 881–922 Occurrence Handle10.1146/annurev.biochem.69.1.881 Occurrence Handle10966478

    Article  PubMed  Google Scholar 

  • S.M. Raja S.S. Rawat A. Chattopadhyay A.K. Lala (1999) ArticleTitleLocalization and environment of tryptophans in soluble and membrane-bound states of a pore-forming toxin from Staphylococcus aureus Biophys. J 76 1469–1479 Occurrence Handle10049328

    PubMed  Google Scholar 

  • K.M. Ranatunga R.J. Law G.R. Smith M.S. Sansom (2001) ArticleTitleElectrostatics studies and molecular dynamics simulations of a homology model of the Shaker K+ channel pore Eur. Biophys. J 30 295–303 Occurrence Handle10.1007/s002490100134 Occurrence Handle11548132

    Article  PubMed  Google Scholar 

  • A.N. Ridder S. Morein J.G. Stam A. Kuhn B. de Kruijff J.A. Killian (2000) ArticleTitleAnalysis of the role of interfacial tryptophan residues in controlling the topology of membrane proteins Biochemistry 39 6521–6528 Occurrence Handle10.1021/bi000073v Occurrence Handle10828968

    Article  PubMed  Google Scholar 

  • T.I. Rokitskaya Y.N. Antonenko E.A. Kotova (1993) ArticleTitleThe interaction of phthalocyanine with planar lipid bilayers—photodynamic inactivation of gramicidin channels FEBS Lett 329 332–335 Occurrence Handle10.1016/0014-5793(93)80248-S Occurrence Handle7689977

    Article  PubMed  Google Scholar 

  • T.I. Rokitskaya Y.N. Antonenko E.A. Kotova (1996) ArticleTitlePhotodynamic inactivation of gramicidin channels: a flash-photolysis study Biochim. Biophys. Acta 1275 221–226 Occurrence Handle8695636

    PubMed  Google Scholar 

  • T.I. Rokitskaya M. Block Y.N. Antonenko E.A. Kotova P. Pohl (2000) ArticleTitlePhotosensitizer binding to lipid bilayers as a precondition for the photoinactivation of membrane channels Biophys. J 78 2572–2580 Occurrence Handle10777753

    PubMed  Google Scholar 

  • T.I. Rokitskaya S.D. Zakharov Y.N. Antonenko E.A. Kotova W.A. Cramer (2001) ArticleTitleTryptophan-dependent sensitized photoinactivation of colicin E1 channels in bilayer lipid membranes FEBS Lett 505 147–150 Occurrence Handle10.1016/S0014-5793(01)02811-3 Occurrence Handle11557058

    Article  PubMed  Google Scholar 

  • W.E. Savige A. Fontana (1977) ArticleTitleModification of tryptophan to oxindolylalanine by dimethyl sulfoxide-hydrochloric acid Methods Enzymol 47 442–453 Occurrence Handle927197

    PubMed  Google Scholar 

  • D.J. Schibli R.F. Epand H.J. Vogel R.M. Epand (2002) ArticleTitleTryptophan-rich antimicrobial peptides: comparative properties and membrane interactions Biochem. Cell Biol 80 667–677 Occurrence Handle10.1139/o02-147 Occurrence Handle12440706

    Article  PubMed  Google Scholar 

  • M. Schiffer C.H. Chang F.J. Stevens (1992) ArticleTitleThe functions of tryptophan residues in membrane proteins Protein Eng 5 213–214 Occurrence Handle1409540

    PubMed  Google Scholar 

  • T. Schirmer T.A. Keller Y.F. Wang J.P. Rosenbusch (1995) ArticleTitleStructural basis for sugar translocation through maltoporin channels at 3.1 A resolution Science 267 512–514 Occurrence Handle7824948

    PubMed  Google Scholar 

  • S.S. Sham S. Shobana L.E. Townsley J.B. Jordan J.Q. Femandez O.S. Andersen D.V. Greathouse J.F. Hinton (2003) ArticleTitleThe structure, cation binding, transport, and conductance of Gly(15)-Gramicidin A incorporated into SDS micelles and PC/PG vesicles Biochemistry 42 1401–1409 Occurrence Handle10.1021/bi0204286 Occurrence Handle12578352

    Article  PubMed  Google Scholar 

  • T.F. Spande B. Witkop Y. Degani A. Patchornik (1970) ArticleTitleSelective cleavage and modification of peptides and proteins Adv. Protein Chem 24 97–260 Occurrence Handle4915251

    PubMed  Google Scholar 

  • S. Srinivasan C.J. Nichols G.M. Lawless R.W. Olsen A.J. Tobin (1999) ArticleTitleTwo invariant tryptophans on the alphal subunit define domains necessary for GABA(A) receptor assembly J. Biol. Chem 274 26633–26638 Occurrence Handle10.1074/jbc.274.38.26633 Occurrence Handle10480864

    Article  PubMed  Google Scholar 

  • S. Stankowski M. Pawlak E. Kaisheva C.H. Robert G. Schwarz (1991) ArticleTitleA combined study of aggregation, membrane affinity and pore activity of natural and modified melittin Biochim. Biophys. Acta 1069 77–86 Occurrence Handle1932053

    PubMed  Google Scholar 

  • B.A. Steer A.R. Merrill (1994) ArticleTitleThe colicin E1 insertion-competent state: detection of structural changes using fluorescence resonance energy transfer Biochemistry 33 1108–1115 Occurrence Handle10.1021/bi00171a009 Occurrence Handle8110742

    Article  PubMed  Google Scholar 

  • M. Straessle G. Stark (1992) ArticleTitlePhotodynamic inactivation of an ion channel: gramicidin A Photochem. Photobiol 55 461–463 Occurrence Handle1373241

    PubMed  Google Scholar 

  • M. Straessle G. Stark M. Wilhelm P. Daumas F. Heitz R. Lazaro (1989) ArticleTitleRadiolysis and photolysis of ion channels formed by analogues of gramicidin A with a varying number of tryptophan residues Biochim. Biophys. Acta 980 305–314

    Google Scholar 

  • H. Suga K. Shirabe T. Yamamoto M. Tasumi M. Umeda C. Nishimura A. Nakazawa M. Nakanishi Y. Arata (1991) ArticleTitleStructural analyses of a channel-forming fragment of colicin E1 incorporated into lipid vesicles: Fourier-transform infrared and tryptophan fluorescence studies J. Biol. Chem 266 13537–13543 Occurrence Handle1713207

    PubMed  Google Scholar 

  • D.P. Tieleman L.R Forrest M.S. Sansom H.J. Berendsen (1998) ArticleTitleLipid properties and the orientation of aromatic residues in OmpF, influenza M2, and alamethicin systems: molecular dynamics simulations Biochemistry 37 17554–17561 Occurrence Handle10.1021/bi981802y Occurrence Handle9860871

    Article  PubMed  Google Scholar 

  • M.C. Tory A.R. Merrill (1999) ArticleTitleAdventures in membrane protein topology. A study of the membrane-bound state of colicin E1 J. Biol. Chem 274 24539–24549 Occurrence Handle10.1074/jbc.274.35.24539 Occurrence Handle10455117

    Article  PubMed  Google Scholar 

  • M.C. Tory A.R. Merrill (2002) ArticleTitleDetermination of membrane protein topology by red-edge excitation shift analysis: application to the membrane-bound colicin E1 channel peptide Biochim. Biophys. Acta 1564 435–448 Occurrence Handle12175927

    PubMed  Google Scholar 

  • D.P. Valenzeno M Tarr (1991) Membrane photomodification and its use to study reactive oxygen effects J.F. Rabek (Eds) Photochemistry and Photophysics CRC Press Boca Raton, Ann Arbor, Boston 137–191

    Google Scholar 

  • P. Van Gelder F. Dumas I. Bartoldus N. Saint A. Prilipov M. Winterhalter Y. Wang A. Philippsen J.P. Rosenbusch T. Schirmer (2002) ArticleTitleSugar transport through maltoporin of Escherichia coli: role of the greasy slide J. Bacterial 184 2994–2999 Occurrence Handle10.1128/JB.184.11.2994-2999.2002

    Article  Google Scholar 

  • B. Vecsey-Semjen C. Lesieur R. Mollby F.G. van der Goot (1997) ArticleTitleConformational changes due to membrane binding and channel formation by staphylococcal alpha-toxin J. Biol. Chem. 272 5709–5717 Occurrence Handle10.1074/jbc.272.9.5709 Occurrence Handle9038182

    Article  PubMed  Google Scholar 

  • G. Verza L. Bakas (2000) ArticleTitleLocation of tryptophan residues in free and membrane bound Escherichia coli alpha-hemolysin and their role on the lytic membrane properties Biochim. Biophys. Acta. 1464 27–34 Occurrence Handle10704917

    PubMed  Google Scholar 

  • M.C. Vissers A.C. Carr A.L. Chapman (1998) ArticleTitleComparison of human red cell lysis by hypochlorous and hypobromous acids: insights into the mechanism of lysis Biochem. J 330 131–138 Occurrence Handle9461501

    PubMed  Google Scholar 

  • B.A. Wallace R.W. Janes (1999) ArticleTitleTryptophans in membrane proteins. X-ray crystallographic analyses Adv. Exp. Med. Biol. 467 789–799 Occurrence Handle10721132

    PubMed  Google Scholar 

  • M.S. Weiss G.E. Schulz (1992) ArticleTitleStructure of porin refined at 1.8 A resolution J. Mol. Biol. 227 493–509 Occurrence Handle10.1016/0022-2836(92)90903-W Occurrence Handle1328651

    Article  PubMed  Google Scholar 

  • S.H. White W.C. Wimley (1998) ArticleTitleHydrophobic interactions of peptides with membrane interfaces Biochim. Biophys. Acta 1376 339–352 Occurrence Handle9804985

    PubMed  Google Scholar 

  • G.A. Woolley B.A. Wallace (1992) ArticleTitleModel ion channels—gramicidin and alamethicin J. Membrane Biol 129 109–136

    Google Scholar 

  • D. Yan M.K. Schulte K.E. Bloom M.M. White (1999) ArticleTitleStructural features of the ligand-binding domain of the serotonin 5HT3 receptor J. Biol. Chem 274 5537–5541 Occurrence Handle10.1074/jbc.274.9.5537 Occurrence Handle10026168

    Article  PubMed  Google Scholar 

  • W.S. Yew H.E. Khoo (2000) ArticleTitleThe role of tryptophan residues in the hemolytic activity of stonustoxin, a lethal factor from stonefish (Synanceja horrida) venom Biochimie 82 251–257 Occurrence Handle10.1016/S0300-9084(00)00203-0 Occurrence Handle10863009

    Article  PubMed  Google Scholar 

  • S.D. Zakharov W.A. Cramer (2002a) ArticleTitleColicin crystal structures: pathways and mechanisms for colicin insertion into membranes Biochim, Biophys. Acta 1565 333–346

    Google Scholar 

  • S.D. Zakharov Cramer.W.A. (2002b) ArticleTitleInsertion intermediates of pore-forming colicins in membrane two-dimensional space Biochimie 84 465–475 Occurrence Handle10.1016/S0300-9084(02)01453-0

    Article  Google Scholar 

  • S.D. Zakharov J.B. Heymann Y.L. Zhang W.A. Cramer (1996) ArticleTitleMembrane binding of the colicin E1 channel: activity requires an electrostatic interaction of intermediate magnitude Biophys. J 70 2774–2783 Occurrence Handle8744315

    PubMed  Google Scholar 

  • S.D. Zakharov M. Lindeberg W.A. Cramer (1999) ArticleTitleKinetic description of structural changes linked to membrane import of the colicin E1 channel protein Biochemistry 38 11325–11332 Occurrence Handle10.1021/bi9903087 Occurrence Handle10471282

    Article  PubMed  Google Scholar 

  • S.D. Zakharov M. Lindeberg Y. Griko Z. Salamon G. Tollin F.G. Prendergast W.A. Cramer (1998) ArticleTitleMembrane-bound state of the colicin E1 channel domain as an extended two-dimensional helical array Proc. Natl. Acad. Sci. USA 95 4282–4287 Occurrence Handle10.1073/pnas.95.8.4282 Occurrence Handle9539728

    Article  PubMed  Google Scholar 

  • S.D. Zakharov T.I. Rokitskaya V.L. Shapovalov Y.N. Antonenko W.A. Cramer (2002) ArticleTitleTuning the membrane surface potential for efficient toxin import Proc. Natl. Acad. Sci. USA 99 8654–8659 Occurrence Handle12060711

    PubMed  Google Scholar 

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Acknowledgements

We are indebted to Prof. Vladimir Skulachev, Moscow State University, for helpful discussion and comments. This work was supported in part by a grant from the NIH: Fogarty Award TW01235 (W.A.C.), and a grant from the RFBR: 03-04-48905 (Y.N.A.).

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Authors and Affiliations

  1. A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russia

    A. A. Sobko, M. A. Vigasina, T. I. Rokitskaya, E. A. Kotova & Y. N. Antonenko

  2. Department of Biological Sciences, Purdue University, West Lafayette, IN, 47907, USA

    S. D. Zakharov & W. A. Cramer

  3. Institute of Basic Problems of Biology, Russian Academy of Sciences, Pouschino, Moscow Region, 142290, Russia

    S. D. Zakharov

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  1. A. A. Sobko
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  2. M. A. Vigasina
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  3. T. I. Rokitskaya
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  4. E. A. Kotova
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  5. S. D. Zakharov
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  6. W. A. Cramer
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  7. Y. N. Antonenko
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Correspondence to Y. N. Antonenko.

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Abbreviations: AlPcS3, almininum trisulfophthalocyanine; BLM, bilayer lipid membrane; DOPC, dioleoylphosphatidylcholine; DOPG, dioleoylphosphatidyl-glycerol; DPhPG, diphytanoylphos-phatidylglycerol; DPhPg, diphytanoylphosphatidylcholine; gA, gramicidin A; NBS, N-bromosuccinimide

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Sobko, A.A., Vigasina, M.A., Rokitskaya, T.I. et al. Chemical and Photochemical Modification of Colicin E1 and Gramicidin A in Bilayer Lipid Membranes. J Membrane Biol 199, 51–62 (2004). https://doi.org/10.1007/s00232-004-0674-y

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  • DOI: https://doi.org/10.1007/s00232-004-0674-y

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