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Liquid temperature measurement method in microchannels by using fluorescence polarization

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Abstract

A novel optical method that can measure fluid temperature at the microscopic scale by measuring fluorescence polarization is described in this paper. The measurement is much less influenced by fluorescence quenching effects, which is a significant issue in conventional laser-induced fluorescence methods. Therefore, the effects of the other properties of the fluid can be reduced considerably in the proposed method, thus has the potential of leading to greater reliability in measuring the temperature. An experiment was performed in a microchannel flow by using fluorescent molecule probes. The relationship between the fluid temperature and the measured fluorescence polarization degree is evaluated to derive the correlation curve. In addition, the effects of the fluid viscosity and fluid pH on the fluorescence polarization degree are discussed to evaluate the influence of the quenching effects. The results showed that the fluorescence polarization is considerably less sensitive to the quenching factors as compared with the fluorescence intensity measurements. Furthermore, a strong linear correlation between the polarization degree and the fluid temperature was obtained. This relationship agreed well with the theoretical one qualitatively and confirmed the validity of the measurements and feasibility of the proposed method.

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References

  1. Petersen NJ, Nikolajsen RPH, Mogensen KB, Kutter JP (2004) Effect of joule heating on efficiency and performance for microchip-based and capillary-based electrophoretic separation systems: a closer look. Electrophoresis 25:253–269

    Article  Google Scholar 

  2. Mao H, Holden MA, You M, Cremer PS (2002) Reusable platforms for high-throughput on-Chip temperature gradient assays. Anal Chem 74:5071–5075

    Article  Google Scholar 

  3. Rebrov EV, Shouten JC, Croon MHJM (2011) Single-phase fluid flow distribution and heat transfer in microstructured reactors. Chem Eng Sci 66:1374–1393

    Article  Google Scholar 

  4. Guijt RM, Dodge A, Dedem GWK, Rooij NF, Verpoorte E (2002) Chemical and physical processes for integrated temperature control in microfluidic devices. Lab Chip 3:1–4

    Article  Google Scholar 

  5. Sun K, Yamaguchi A, Ishida Y, Matsuo S, Misawa H (2002) A heater-integrated transparent microchannel Chip for continuous-flow PCR. Sensors Actuators B 84:283–289

    Article  Google Scholar 

  6. Kim YH, Yang I, Bae YS, Park SR (2008) Performance evaluation of thermal cyclers for PCR in a rapid cycling condition. BioTechniques 44:495–505

    Article  Google Scholar 

  7. Liao C-S, Lee G-B, Wu J-J, Chang C-C, Hsieh T-M, Huang F-C, Luo C-H (2005) Micromachined polymerase chain reaction system for multiple DNA amplification of upper respiratory tract infectious diseases. Biosens Bioelectron 20:1341–1348

    Article  Google Scholar 

  8. Chen P-C, Nikitopoulus DE, Soper SA, Murphy MC (2008) Temperature distribution effects on micro-CFPCR performance. Biomed Microdevices 10:141–152

    Article  Google Scholar 

  9. Lund-Olesen T, Dufva M, Dahl JA, Collas P, Hansen MF (2008) Sensitive on-chip quantitative real-time PCR performed on an adaptable and robust platform. Biomed Microdevices 10:769–776

    Article  Google Scholar 

  10. Debby D, Bluhm R, Habets N, Kurz H (1997) Fabrication of planar thermocouples for real time measurement of temperature profiles in polymer melts. Sensors Actuators 58:179–184

    Article  Google Scholar 

  11. Glasser H, Schnelle T, Müller T, Fuhr G (1999) Electric field calibration in micro-electrode chambers by temperature measurements. Thermochimca Acta 333:183–190

    Article  Google Scholar 

  12. Ross D, Gaitan M, Locascio LE (2001) Temperature measurement in microfluidic systems using a temperature-dependent fluorescent dye. Anal Chem 73:4117–4123

    Article  Google Scholar 

  13. Yoon SY, Kim KC (2006) Signal intensity enhancement of μ-LIF by using ultra-thin laser illumination and aqueous mixture with ethanol/methanol for micro-channel applications. Opt Lasers Eng 44:224–239

    Article  Google Scholar 

  14. Natrajan VK, Christensen KT (2009) Two-color laser-induced fluorescent thermometry for microfluidic systems. Meas Sci Technol 20:1–11

    Article  Google Scholar 

  15. Jaboński A (1960) On the notion of emission anisotropy. Bulletin of the Polish Academy of Sciences Series A 8:259–264

    Google Scholar 

  16. Arik M, Celebi N, Onganer Y (2005) Fluorescence quenching of fluorescein with molecular oxygen in solution, journal of photochemistry and photobiology. Journal of Photochemistry and Photobiology 170:105–111

    Article  Google Scholar 

  17. Song L, Hennik EJ, Young IT, Tanke HJ (1995) Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy. Biophys J 68:2588–2600

    Article  Google Scholar 

  18. Lakowicz JR (2004) Principles of fluorescence spectroscopy, 2nd edn. Springer Science + Business Media Inc., New York

  19. Guilbault G G (1990) Practical Fluorescence, 2nd edn, revised and expanded. Marcel Dekker Inc., New York

  20. Perrin F (1929) La Fluorescence des Solutions. Induction Moléculaire – Polarisation et Durée D’émission – Photochimie Ann de Physique 12:169–275

    Google Scholar 

  21. Weber G (1971) Theory of fluorescence depolarization by anisotropic Brownian rotations. J Chem Phys 55:2399–2407

    Article  Google Scholar 

  22. Fowlkes JD (2006) Molecular transport in a crowded volume created from vertically aligned carbon Nanafibres: a fluorescence recovery after Photobleaching study. Nanotechnology 17:5659–5668

    Article  Google Scholar 

  23. Farrell HM, Kumosinski TF, Cooke PH, King G, Hoagland PD, Wickham ED, Dower HJ, Groves ML (1996) Particle sizes of purified κ-casein: metal effect and correspondence with predicted three-dimensional molecular models. J Protein Chem 15:435–446

    Article  Google Scholar 

  24. Obare SO (2010) Fluorescent Chemosensors for toxic organophosphorus. Sensors 10:7018–7043

    Article  Google Scholar 

  25. Magde D (1999) Solvent dependence of the fluorescence lifetimes of xanthene dyes. Photochem Photobiol 70:734–744

    Article  Google Scholar 

  26. Kawski A (1983) Excitation energy transfer and its manifestation in isotropic media. Photochem Photobiol 38:487–508

    Article  Google Scholar 

  27. ASME Measurement Uncertainty, Performance Test Codes ANSI/ASME PTC 19.1–1985. The American Society of Mechanical Engineers, New York

  28. Liu W-T, Wu J-H, Li ES-Y, Selamat ES (2005) Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies. Appl Environ Microbiol 71:6453–6457

    Article  Google Scholar 

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Acknowledgments

This work was partially supported by the Japan Society for the Promotion of Science KAKENHI Grant Number 15H03931, SPIRITS (Supporting Program for Interaction-based Initiative Team Studies), and Micro/Nano Fabrication Hub in Kyoto University of “Low-Carbon Research Network” funded by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

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Correspondence to Kazuya Tatsumi.

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Tatsumi, K., Hsu, C.H., Suzuki, A. et al. Liquid temperature measurement method in microchannels by using fluorescence polarization. Heat Mass Transfer 54, 2607–2616 (2018). https://doi.org/10.1007/s00231-017-2104-6

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  • DOI: https://doi.org/10.1007/s00231-017-2104-6

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