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Marine Biology

, Volume 132, Issue 3, pp 495–501 | Cite as

Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fluorescent in situ hybridization

  • F. Fontana
  • J. Tagliavini
  • L. Congiu
  • M. Lanfredi
  • M. Chicca
  • C. Laurente
  • R. Rossi
Article

Abstract

A karyotype analysis by several staining techniques was carried out on the great sturgeon, Huso huso (Linnaeus, 1758). The karyotype (2n = 118 ± 2) was composed of 42 pairs of meta-/submetacentric chromosomes and 17 pairs of acrocentrics/microchromosomes. Constitutive heterochromatin was mainly located at the centromeric regions of the acrocentric chromosomes. The biarmed chromosomes showed weak C-bands. Fluorescent staining with GC-specific chromomycin A3 showed clearly recognizable fluorescent regions, whereas a more uniform base composition was revealed by the AT-specific 4,6-diamidino-2 phenylindole. After Ag-staining, nucleolar organizer regions could be observed on the short arms of two medium-sized submetacentrics and on two acrocentrics. Digoxigenated 28S and 5S rDNA probes, prepared from Acipenser naccarii DNA and hybridized to metaphase chromosomes, showed signals on six and two chromosomes, respectively. The telomeric sequence (TTAGGG) n detected by FISH was located at both ends of each chromosome. Results are discussed in relation to karyotype organization and evolution in sturgeons.

Keywords

Centromeric Region Staining Technique Karyotype Analysis Nucleolar Organizer Region Nucleolar Organizer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • F. Fontana
    • 1
  • J. Tagliavini
    • 2
  • L. Congiu
    • 1
  • M. Lanfredi
    • 1
  • M. Chicca
    • 1
  • C. Laurente
    • 1
  • R. Rossi
    • 1
  1. 1.Department of Biology, University of Ferrara, Via L. Borsari, 46, I-44100 Ferrara, ItalyIT
  2. 2.Department of Evolutionary Biology, University of Parma, Via delle Scienze, I-43100 Parma, ItalyIT

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