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Marine Biology

, Volume 136, Issue 5, pp 823–827 | Cite as

Humoral opsonin from the colonial ascidian Botryllus schlosseri as a member of the galectin family

  • L. Ballarin
  • C. Tonello
  • A. Sabbadin

Abstract

The synthesis and secretion of the humoral opsonin from the colonial ascidian Botryllus schlosseri was studied by using specific polyclonal antibodies that recognise both of the bands obtained after SDS-PAGE of the purified lectin. The agglutinin was revealed on the surface of agglutinated yeast and red blood cells and in the cytoplasm of the phagocytes, either uniformly distributed or localised inside the cytoplasmic projections. A previous incubation of haemocytes with the agglutinin resulted in intense labelling of the surface that disappeared in the presence of D-galactose, whereas it was not affected by treatment with proteases. These results, together with the already published data, suggest that our molecule is a new member of the galectin family.

Keywords

Blood Cell Polyclonal Antibody Intense Labelling Cytoplasmic Projection Specific Polyclonal Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • L. Ballarin
    • 1
  • C. Tonello
    • 1
  • A. Sabbadin
    • 1
  1. 1.Dipartimento di Biologia, Università di Padova, Via U. Bassi 58/B, I-35121 Padova, ItalyIT

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