Abstract
Marine flatfishes have a low metabolic rate and routinely encounter large fluctuations in salinity, and are therefore of interest in the study of diffusive water flux (a proxy for transcellular water permeability), oxygen consumption (ṀO2), ammonia excretion and urea-N excretion as a function of salinity and seawater [Ca2+]. These parameters were measured in two coastal marine flatfishes, Pacific sanddab and Rock sole acclimated to 31 ppt and exposed acutely (for up to 3 h), to environmentally relevant salinities of 45, 15.5, or 7.5 ppt. In both species, diffusive water flux and ammonia excretion rates increased as salinity decreased. ṀO2 and urea-N excretion rates remained relatively unchanged. Nitrogen quotient analysis indicated increased oxidation of protein at lower salinity. A second experimental series was performed on Rock sole to separate the effects of salinity from those of ambient [Ca2+]. In direct contrast to the significant increase seen at 7.5 ppt, reducing salinity from 31 ppt to 7.5 ppt while maintaining [Ca2+] at 10 mM or increasing it to 20 mM resulted in no change in diffusive water flux rate, demonstrating that reduced [Ca2+], rather than reduced salinity itself, is the primary cause for the increases in diffusive water flux. However, ammonia excretion rate increased when salinity was decreased and [Ca2+] was increased compared to 31 ppt with added [Ca2+]. Our results demonstrate that both diffusive water flux and ammonia excretion rates are a function of salinity, that neither are coupled to ṀO2, and that ambient [Ca2+] also plays a role in these rates.
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Acknowledgements
We thank the Bamfield Marine Sciences Centre staff, especially the Research Co-ordinator, Tao Eastham, for excellent assistance, and two anonymous reviewers whose constructive comments improved the paper.
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Supported by an NSERC (Canada) Discovery Grant (RGPIN-2017–03843) to CMW.
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The project was jointly conceived by CM and CMW. CM did the experiments under the supervision of CMW. CM wrote the first draft of the paper, and CMW edited it.
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Animals were collected under Fisheries and Oceans Canada collection permit XR 136 2021. The University of British Columbia Animal Care Committee (AUP A18-0271) and the Bamfield Marine Science Centre Animal Care Committee (AUP RS-21(19)-01)) approved all experimental procedures, in accordance with the Canadian Council on Animal Care guidelines.
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Morris, C., Wood, C.M. The effect of salinity and calcium on diffusive water flux, oxygen consumption, and nitrogenous waste excretion rates in Pacific sanddab (Citharichthys sordidus) and Rock sole (Lepidopsetta bilineata). Mar Biol 170, 108 (2023). https://doi.org/10.1007/s00227-023-04245-w
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DOI: https://doi.org/10.1007/s00227-023-04245-w