Abstract
The interaction between bacteria and phytoplankton during bloom events is complicated throughout the developmental processes of algal blooms. The detailed ecological roles of bacterioplankton during algal blooms still need to be investigated comprehensively. With the assistance of omics techniques, the composition and function of bacterioplankton were studied during the blooming and recession periods of Phaeocystis globosa in the Beibu Gulf, China. The transcriptionally active taxa of Vibrio, which correlated with most functional genes, were enriched in the blooming period, whereas the active microbial groups, such as Erythrobacter and Candidatus puniceispirillum, increased significantly in the recession period of the P. globosa bloom. The transcriptional analyses indicated the blooming and recession periods of P. globosa had different impacts on the function of bacterioplankton, including shifts in expression of specific metabolic pathways for variable nutritional utilization and their advantage in bacterial motility, quorum sensing, and extracellular secretion. Overall, the integrated field investigation and in-depth analysis of molecular data highlighted the difference of bacterial community during the outbreak and collapse stages of P. globosa bloom, and provided fundamental data for better understanding of the bacterial community in response to blooming and recession of the P. globosa bloom.
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Data availability
All environmental datasets are publicly available from the sources identified in the manuscript. The raw data of metagenomic and metatranscriptomic sequencing have been deposited in the Sequence Read Archive (NCBI) under Accession Number PRJNA678802 and PRJNA686139, respectively.
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Acknowledgements
The hospitality and assistance of R/V Haishun No.6 crew are gratefully acknowledged. We thank the team of Prof. Yu Zhiming at IOCAS for DO, pH, five inorganic nutrients and organic carbon measurements, and the team of Prof. Guan Weibing at SIO, MNR for temperature and salinity measurements.
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This study was supported by the National Key Research and Development Program (Grant No. 2017YFC1404300) of the Ministry of Science and Technology, China, the Scientific and Technological Innovation Project (Grant No. 2016ASKJ02) and the Aoshan Talents Program (Grant No. 2015ASTP) supported by The Pilot National Laboratory for Marine Science and Technology (Qingdao).
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The study was conceived and designed by SX, CL and SS. Data collection was performed by SX, CH, and ZK. Data preparation, processing, and analysis were performed by SX. The first draft of the manuscript was written by SX, and all authors contributed to subsequent versions of the manuscript. All authors read and approved the final manuscript.
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Xu, S., He, C., Kang, Z. et al. The ecological responses of bacterioplankton during a Phaeocystis globosa bloom in Beibu Gulf, China highlighted by integrated metagenomics and metatranscriptomics. Mar Biol 169, 36 (2022). https://doi.org/10.1007/s00227-022-04021-2
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DOI: https://doi.org/10.1007/s00227-022-04021-2