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Tracing European eel in the diet of mesopelagic fishes from the Sargasso Sea using DNA from fish stomachs

Abstract

Trophic interactions in complex mesopelagic ecosystems are generally poorly understood, but tracing diet remains of predators provides key insights into these. In many cases, however, the prey remains obtained from predator stomachs are unidentifiable by visual inspection and identification depends on new molecular techniques. Here, we search for predators on larvae of the critically endangered European eel (Anguilla anguilla), a species which has shown a dramatic decline in recruitment to the stock. We sampled for predators among mesopelagic fishes using pelagic trawling in the known area of spawning and larval distribution of A. anguilla in the Sargasso Sea. We aimed at developing a species-specific quantitative PCR (qPCR) system, targeting the nd4-region of mitochondrial DNA (mtDNA), to search for eel remains in the stomachs of 17 mesopelagic fish taxa (62 specimens). Remains of A. anguilla were confirmed in 9.7% of all fish stomachs investigated, representing six species (Lampanyctus cuprarius, L. photonotus, Myctophum selenops, Notoscopelus caudispinosus, Melamphaes typhlops, and Chauliodus danae). Thus, our study documents that mesopelagic fishes in the Sargasso Sea to some extent predate the A. anguilla larvae, motivating further studies on upper-level trophic interactions in this oceanic ecosystem.

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Acknowledgements

The Sargasso-eel expedition was funded by the Carlsberg foundation. We are grateful to all crew and staff onboard R/V Dana that helped with the fishing and sorting of the catches. The study was partly funded by the Innovation Fund Denmark (Grant J.nr. 104-2012-1) and the Natural History Museum of Denmark. We thank Werner Schwarzhans for cross-checking fish species identifications using both otolith structures and morphology. We also thank Mikkel Skovrind and Eva Egelyng Sigsgaard for laboratory assistance. Lastly, we thank the three reviewers for their valuable input and suggestions to the manuscript.

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Correspondence to Mads Reinholdt Jensen.

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All applicable international and national guidelines for the care and use of animals were followed. All procedures performed in this study were in accordance with the ethical standards of the institution at which the study was conducted.

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The authors declare no financial or other conflict of interests.

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Responsible Editor: M. Rius.

Reviewed by N. A. Hardy, H. Krehenwinkel and an undisclosed expert.

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Jensen, M.R., Knudsen, S.W., Munk, P. et al. Tracing European eel in the diet of mesopelagic fishes from the Sargasso Sea using DNA from fish stomachs. Mar Biol 165, 130 (2018). https://doi.org/10.1007/s00227-018-3390-3

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  • DOI: https://doi.org/10.1007/s00227-018-3390-3