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Marine Biology

, 164:71 | Cite as

New tool to elucidate the diet of the ormer Haliotis tuberculata (L.): Digital shell color analysis

  • V. MarchaisEmail author
  • A. Jolivet
  • S. Hervé
  • S. Roussel
  • B. R. Schöne
  • J. Grall
  • L. Chauvaud
  • J. Clavier
Original paper

Abstract

Food sources of the European abalone Haliotis tuberculata throughout its life cycle are still to be clarified in nature. A novel non-destructive method of digital shell color analysis to reveal the diets of European abalone (ormer) was developed in this study. The method was calibrated using ormers reared under experimental conditions in North Western Brittany in 2012 and fed a controlled monospecific diet to define the shell hues associated with various macroalgae (i.e., Rhodophyta, Chlorophyta, and Phaeophyta). General food preferences were established by comparing the shell hue of wild adult ormers and experimental adult ormers. Shell hue corresponds to the color tint in the HSL color space measured on digital pictures of the shell. Experimentally, shell hue values differed according to treatment, with the most yellow-green hue (72°) for ormers fed Saccharina sp. and the coral hue (25°) for ormers fed Palmaria palmata. High variation in shell color of wild ormers was observed according to the sampling site and/or ontogeny. The diet of wild ormers may be related to the abundance of different drifting algae in their respective habitats. Thus, this non-destructive and easy-to-use technique appears to be a promising tool for determining the diet of Haliotis species and, perhaps, other herbivorous mollusks.

Keywords

Numerical color Shell hue HSL color space Abalone Food sources Experiment 

Notes

Acknowledgements

We thank the team at the France Haliotis hatchery and their interns for the care of the animals and assistance during the experiment. Many thanks to all of the people who contributed to sampling macroalgae on foreshore. The authors also thank the scuba divers for sampling wild abalones, and Sylvain Huchette for contributing to this experiment and his feedback. We would like to thank the three anonymous reviewers for their helpful comments.

Compliance with ethical standards

The project was supported by the National Research Agency, French Government, with regard to investment expenditure program PIA-ANR IDEALG BTBR-10-04 and the CHIVAS (ANR-Blanc) program. V. Marchais was supported by a doctoral grant from the Université de Bretagne Occidentale. All applicable international and national guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

Conflict of interest

Authors declare that they have no conflict of interest.

Supplementary material

227_2017_3103_MOESM1_ESM.pdf (173 kb)
Supplementary material 1 (PDF 173 KB)
227_2017_3103_MOESM2_ESM.pdf (7.8 mb)
Supplementary material 2 (PDF 7950 KB)
227_2017_3103_MOESM3_ESM.pdf (1.1 mb)
Supplementary material 3 (PDF 1156 KB)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Laboratoire des Sciences de l’Environnement Marin (LEMAR)UMR 6539 UBO, CNRS, IRD, IFREMER, Institut Universitaire Européen de la Mer (IUEM)PlouzanéFrance
  2. 2.TBM Environnement/ SommeTechnopole Brest IroisePlouzanéFrance
  3. 3.Institut Universitaire Européen de la Mer (IUEM)University of Brest, UMS 3113PlouzanéFrance
  4. 4.Institute of GeosciencesUniversity of MainzMainzGermany
  5. 5.Observatoire des Sciences de l’UniversUMS 3113, Institut Universitaire Européen de la Mer (IUEM)PlouzanéFrance
  6. 6.BeBEST Laboratoire International AssociéPlouzanéFrance

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