Marine Biology

, 163:146 | Cite as

Predicting in vivo oxygen consumption rate from ETS activity and bisubstrate enzyme kinetics in cultured marine zooplankton

  • N. Osma
  • I. Fernández-Urruzola
  • M. Gómez
  • S. Montesdeoca-Esponda
  • T. T. Packard
Original paper


Oxygen consumption rates (\(R_{{{\text{O}}_{2} }}\)) in the rotifer Brachionus plicatilis and the mysid Leptomysis lingvura during both well-fed conditions and starvation have been modeled using the electron transport system (ETS) activity, bisubstrate kinetics and intracellular concentration of substrates. Furthermore, the influence of the food quality on the respiratory metabolism and metabolite levels has been explored. The highest values of most of the variables both in rotifers and mysids were mainly found on organisms grown on the lipid-rich diet, although no differences were determined between treatments in the response to starvation. Time courses of the \(R_{{{\text{O}}_{2} }}\) and the concentration of pyridine and adenine nucleotides evidenced a sharp decrease during food shortage and a fast recovery with food restoration, whereas the potential respiration (Φ) remained fairly constant. In general, the modeled \(R_{{{\text{O}}_{2} }}\) (\(V_{{{\text{O}}_{2} }}\)) predicted with a high degree of success in the in vivo \(R_{{{\text{O}}_{2} }}\), even though it yielded relatively lower values. Nonetheless, the correlation of the measured \(R_{{{\text{O}}_{2} }}\) with \(V_{{{\text{O}}_{2} }}\) during starvation was much better than with the \(R_{{{\text{O}}_{2} }}\) estimated from ETS measurements and a fixed \(R_{{{\text{O}}_{2} }}\)/Φ ratio. Finally, the observed relationship between the measured \(R_{{{\text{O}}_{2} }}\) and the adenine nucleotide ADP suggests that the contribution of this nucleotide should be included in future applications of this model.


Kinetic Constant Intracellular Concentration Oxygen Consumption Rate Electron Transport System Pyridine Nucleotide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We are grateful to CM Hernández and D López for kindly providing the cultures of Nannochloropsis sp. and B. plicatilis from their culturing collection. Thanks are extended to JJ Santana–Rodríguez for enabling us to perform the HPLC analysis in his laboratory. We would like to thank the anonymous reviewers for contributing valuables suggestions that notably improved the manuscript. This work was funded by the BIOMBA project (CTM2012-32729/MAR) awarded to M Gómez by the Spanish Ministry of Economy and Competitiveness. N Osma and I Fernández–Urruzola received financial support from the Formation and Perfection of the Researcher Personal Program from the Basque Government. TT Packard was largely supported by TIAA-CREF and Social Security (USA).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • N. Osma
    • 1
  • I. Fernández-Urruzola
    • 1
  • M. Gómez
    • 1
  • S. Montesdeoca-Esponda
    • 2
  • T. T. Packard
    • 1
  1. 1.Marine Ecophysiology Group (EOMAR)Universidad de Las Palmas de Gran CanariaLas Palmas De Gran CanariaSpain
  2. 2.Environmental Chemical Analysis GroupUniversidad de Las Palmas de Gran CanariaLas Palmas De Gran CanariaSpain

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