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Marine Biology

, 163:134 | Cite as

High-temperature acclimation strategies within the thermally tolerant endosymbiont Symbiodinium trenchii and its coral host, Turbinaria reniformis, differ with changing pCO 2 and nutrients

  • Kenneth D. Hoadley
  • D. Tye Pettay
  • Andréa G. Grottoli
  • Wei-Jun Cai
  • Todd F. Melman
  • Stephen Levas
  • Verena Schoepf
  • Qian Ding
  • Xiangchen Yuan
  • Yongchen Wang
  • Yohei Matsui
  • Justin H. Baumann
  • Mark E. Warner
Original paper

Abstract

The dinoflagellate Symbiodinium trenchii associates with a wide array of host corals throughout the world, and its thermal tolerance has made it of particular interest within the context of reef coral resilience to a warming climate. However, future reefs are increasingly likely to face combined environmental stressors, further complicating our understanding of how S. trenchii will possibly acclimatize to future climate scenarios. Over a 33-day period, we characterized the individual and combined affects of high temperature (26.5 vs. 31.5 °C), pCO2 (400 vs. 760 µatm), and elevated nutrients (0.4 and 0.2 vs. 3.5 and 0.3 µmol of NO3/NO2 and \({\text{PO}}_{4}^{3 - }\), respectively) on S. trenchii within the host coral species Turbinaria reniformis. Global analysis across all treatments found temperature to be the largest driver of physiological change. However, exposure to elevated temperature led to changes in symbiont physiology that differed across pCO2 concentrations. Net photosynthesis and cellular chlorophyll a increased with temperature under ambient pCO2, whereas temperature-related differences in cellular volume and its affect on pigment packaging were more pronounced under elevated pCO2. Furthermore, increased nutrients mitigated the physiological response to high temperature under both ambient and elevated pCO2 conditions and represented a significant interaction between all three physical parameters. Individual responses to temperature and pCO2 were also observed as cellular density declined with elevated temperature and calcification along with respiration rates declined with increased pCO2. Symbiodinium trenchii remained the dominant symbiont population within the host across all treatment combinations. Our results reveal distinct physiological changes in response to high temperature within the S. trenchii/T. reniformis symbioses that are dependent on pCO2 and nutrient concentration, and represent important interactive effects to consider as we consider how corals will respond under future climate change scenarios.

Keywords

Dissolve Inorganic Carbon PSII Reaction Center High pCO2 Elevated pCO2 pCO2 Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank the employee’s at Reef Systems Coral Farm for their assistance with maintaining coral colonies prior to experimentation and with construction of the experimental systems. The work was funded by the National Science Foundation, Grant Nos. 1041124 and 1316055.

Supplementary material

227_2016_2909_MOESM1_ESM.pdf (165 kb)
Supplementary material 1 (PDF 165 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Kenneth D. Hoadley
    • 1
  • D. Tye Pettay
    • 1
  • Andréa G. Grottoli
    • 2
  • Wei-Jun Cai
    • 1
    • 3
  • Todd F. Melman
    • 4
  • Stephen Levas
    • 2
    • 8
  • Verena Schoepf
    • 2
    • 7
  • Qian Ding
    • 3
    • 9
  • Xiangchen Yuan
    • 5
  • Yongchen Wang
    • 3
  • Yohei Matsui
    • 2
  • Justin H. Baumann
    • 2
    • 6
  • Mark E. Warner
    • 1
  1. 1.School of Marine Science and PolicyUniversity of DelawareLewesUSA
  2. 2.School of Earth SciencesThe Ohio State UniversityColumbusUSA
  3. 3.Department of Marine SciencesUniversity of GeorgiaAthensUSA
  4. 4.Reef Systems Coral FarmNew AlbanyUSA
  5. 5.Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  6. 6.Department of Marine SciencesUniversity of North CarolinaChapel HillUSA
  7. 7.ARC Centre of Excellence for Coral Reef Studies, UWA Oceans Institute and School of Earth and EnvironmentUniversity of Western AustraliaCrawleyAustralia
  8. 8.Department of Geography and Environmental ScienceVillanova UniversityVillanovaUSA
  9. 9.Ocean CollegeZhejiang UniversityHangzhouChina

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